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Q5WFG8 (MURD_BACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:ABC2357
OrganismBacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]
Taxonomic identifier66692 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length451 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 451451UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108963

Regions

Nucleotide binding118 – 1247ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q5WFG8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 1FCB2C4FEBE6DB69

FASTA45148,186
        10         20         30         40         50         60 
MKQVKELIGK RVLVLGMAKS GVASALLLAR IGAEVVINDS KSRADQPQAG ELEAAGIQVV 

        70         80         90        100        110        120 
CGGHPLTVLD GCSLLVKNPG IPYTNIVVKE AEARGIPIWT EIELAYLISE AEMVAITGSN 

       130        140        150        160        170        180 
GKTTTTTLVK EMLEHSGRKP LIAGNIGTVA SEVAQKAKAE HVIVLEVSSF QLMGTNAFQP 

       190        200        210        220        230        240 
KVAVWLNIFD AHLDYHGTRE DYIAAKARIA ANMGPADYLV YNADDPTVVQ AIARIGATLV 

       250        260        270        280        290        300 
PFSRINVVED GAYVKDGTIF FKDEPILALA DAVLPGAHNV ENMLAATAAA RLAGATVEQI 

       310        320        330        340        350        360 
RHVLSHFPGV KHRLQYVGSW EGRQFYNDSK ATNILATKAA LSGFAKPVVL IAGGLDRGND 

       370        380        390        400        410        420 
FDELLASLKY VKAVVAYGET KSKLLALAAK ANVQAVTAER VQDATEKAVA LSQPGDVVLL 

       430        440        450 
SPACASWDQY RSFEERGDEF LDYVNTIIKP S

« Hide

References

[1]"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KSM-K16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006627 Genomic DNA. Translation: BAD64892.1.
RefSeqYP_175853.1. NC_006582.1.

3D structure databases

ProteinModelPortalQ5WFG8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5WFG8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000046664; EBBACP00000045425; EBBACG00000046655.
GeneID3204495.
GenomeReviewsGene locus ABC2357 in contig AP006627_GR.
KEGGbcl:ABC2357.
NMPDRfig|66692.3.peg.2045.
PATRIC18924304. VBIBacCla58185_2515.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0771.
GeneTreeEBGT00070000032130.
HOGENOMHBG750024.
OMAACASWDM.
ProtClustDBPRK02472.

Enzyme and pathway databases

BioCycBCLA66692:ABC2357-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BACSK
AccessionPrimary (citable) accession number: Q5WFG8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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