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Q5WFA2

- DEF_BACSK

UniProt

Q5WFA2 - DEF_BACSK

Protein

Peptide deformylase

Gene

def

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi110 – 1101IronUniRule annotation
    Metal bindingi153 – 1531IronUniRule annotation
    Active sitei154 – 1541UniRule annotation
    Metal bindingi157 – 1571IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBCLA66692:GHMP-2491-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
    Short name:
    PDFUniRule annotation
    Alternative name(s):
    Polypeptide deformylaseUniRule annotation
    Gene namesi
    Name:defUniRule annotation
    Ordered Locus Names:ABC2423
    OrganismiBacillus clausii (strain KSM-K16)
    Taxonomic identifieri66692 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001168: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Peptide deformylasePRO_0000301002Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi66692.ABC2423.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5WFA2.
    SMRiQ5WFA2. Positions 1-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0242.
    HOGENOMiHOG000243507.
    KOiK01462.
    OMAiHIDKENP.
    OrthoDBiEOG6PZXGQ.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5WFA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITMDDIVRE GNPILRKVAE PVELPLTDED KQTLVDMLEF IKNSQDPEIA    50
    EKYGLRPGVG LAAPQIGISK RLFAIHATDE NGNLYSMGIA NPKVVSHSVE 100
    TNELENGEGC LSVDRDVPGL VPRRARLTIT GVDHEGNDVR MRLRGYIAIV 150
    FQHELDHLDG IMFYDRIEGL EDPFKKPLPG FAL 183
    Length:183
    Mass (Da):20,388
    Last modified:November 23, 2004 - v1
    Checksum:i8A3009F0269B3B8F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006627 Genomic DNA. Translation: BAD64958.1.
    RefSeqiYP_175919.1. NC_006582.1.

    Genome annotation databases

    EnsemblBacteriaiBAD64958; BAD64958; ABC2423.
    GeneIDi3201620.
    KEGGibcl:ABC2423.
    PATRICi18924442. VBIBacCla58185_2584.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006627 Genomic DNA. Translation: BAD64958.1 .
    RefSeqi YP_175919.1. NC_006582.1.

    3D structure databases

    ProteinModelPortali Q5WFA2.
    SMRi Q5WFA2. Positions 1-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 66692.ABC2423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD64958 ; BAD64958 ; ABC2423 .
    GeneIDi 3201620.
    KEGGi bcl:ABC2423.
    PATRICi 18924442. VBIBacCla58185_2584.

    Phylogenomic databases

    eggNOGi COG0242.
    HOGENOMi HOG000243507.
    KOi K01462.
    OMAi HIDKENP.
    OrthoDBi EOG6PZXGQ.

    Enzyme and pathway databases

    BioCyci BCLA66692:GHMP-2491-MONOMER.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
      Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KSM-K16.

    Entry informationi

    Entry nameiDEF_BACSK
    AccessioniPrimary (citable) accession number: Q5WFA2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3