Reviewed, UniProtKB/Swiss-Prot Q5WF63 (DXS_BACSK)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	ABC2462

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256379

Sequences

	Sequence		Length	Mass (Da)
	Q5WF63-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F094A8DB99BC2621	FASTA	628	69,022
	10 20 30 40 50 60 MNLEEIQDPS FLKNYSNKQL EELAADIRRF LIEKLSATGG HIGPNLGVVE LTLALHQLFD 70 80 90 100 110 120 SPKDKLLWDV GHQAYVHKIL TGRAPQFDTL RQYKGLCGFP KRTESEHDVW ETGHSSTSLS 130 140 150 160 170 180 GAMGMATARD LKGTNENIVA IIGDGALTGG MALEALNHIG HEQKHLIVVL NDNEMSIAPN 190 200 210 220 230 240 VGALHSILGR LRTAGKYQKA KEDLETIIRK IPAFGGKLAD TAERLKDSLK YLMVSGMFFE 250 260 270 280 290 300 EMGFTYLGPV DGHDLDDLKH QLSYAKKTSG PVLVHVLTKK GKGYKPAEED ATGAWHGTGP 310 320 330 340 350 360 YKMESGEMVK KPGPPSYPSV FANTLKRIAR EDERVVAITP AMPGGSKLDL FAEEFPDRMF 370 380 390 400 410 420 DVGIAEQHAA TMSAGLATQG MKPVYAVYST FLQRGYDQVV HDICRQNLNV LIAIDRAGLV 430 440 450 460 470 480 GADGETHQGV FDIAFLRSLP NITILNPKDE NEFQHMLYTG ICYDDGPIAI RYPRGNGTGV 490 500 510 520 530 540 KMDASLSRLP IGKWNVLQEG TDAAILTFGT MIPVAEKALA QLKQAGYNIR LINANSVKPL 550 560 570 580 590 600 DEKLLEALAE EELPLLTIEE SVLQGGFGSA VLEFYNEKGM HVELKRMGIP DYFVEHGSVS 610 620 ELYQEVGLTP ERIADTLISM LPQKRKRA

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD64997.1.
RefSeq	YP_175958.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WF63.
Genome annotation databases
GeneID	3202339.
GenomeReviews	Gene locus ABC2462 in contig AP006627_GR.
KEGG	bcl:ABC2462.
NMPDR	fig|66692.3.peg.2150.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WF63.
OMA	MQELPHP.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2462-MON.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACSK

Accession

Primary (citable) accession number: Q5WF63

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)