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Q5WEN5

- LEUC_BACSK

UniProt

Q5WEN5 - LEUC_BACSK

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi346 – 3461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi406 – 4061Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi409 – 4091Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciBCLA66692:GHMP-2719-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:ABC2640
    OrganismiBacillus clausii (strain KSM-K16)
    Taxonomic identifieri66692 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001168: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4704703-isopropylmalate dehydratase large subunitPRO_0000076697Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi66692.ABC2640.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5WEN5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiQARAKTM.
    OrthoDBiEOG600DP5.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5WEN5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTIIEKIW ESHTVVDESG KPNLLYVDLH MVHEVTSPQA FEGLRLAGRT    50
    VRRPDKTFAT MDHNVPTVNR YDIRDQIART QMETLAANCK QFGIEMVDLD 100
    HPENGIVHVI GPELGLTQPG HVIVCGDSHT STHGAFGAIA FGIGTSEVEH 150
    VLATQTIWQS KPKTLEVHIS GRLNEGISAK DVILAIIAKF GVDIGTGSII 200
    EFTGEAIRGM TMEERMTICN MSIEAGAKAG LISPDQVTFD YLQGRANVPK 250
    GEAYDEIVEK WAQLATDPGA TYDRSVTIDA AEIEPMVTWG TNPGQGAGIS 300
    KAVPRLDDAK SEVERRAISQ ALDYMDIKPG TPLSEIEIQH VFIGSCTNSR 350
    LSDLRAAAAV INGRKVAPGV RALIVPGSQR VKKRAEEEGL DQVFKEAGFE 400
    WRDSGCSMCL SMNPDVVPEG ERCASTSNRN FEGRQGKGAR THLVSPAMAA 450
    AAAIAGRFVD VRKMVKAGEC 470
    Length:470
    Mass (Da):50,857
    Last modified:November 23, 2004 - v1
    Checksum:i6488DD9B8A8426D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006627 Genomic DNA. Translation: BAD65175.1.
    RefSeqiYP_176136.1. NC_006582.1.

    Genome annotation databases

    EnsemblBacteriaiBAD65175; BAD65175; ABC2640.
    GeneIDi3204058.
    KEGGibcl:ABC2640.
    PATRICi18924926. VBIBacCla58185_2815.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006627 Genomic DNA. Translation: BAD65175.1 .
    RefSeqi YP_176136.1. NC_006582.1.

    3D structure databases

    ProteinModelPortali Q5WEN5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 66692.ABC2640.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD65175 ; BAD65175 ; ABC2640 .
    GeneIDi 3204058.
    KEGGi bcl:ABC2640.
    PATRICi 18924926. VBIBacCla58185_2815.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi QARAKTM.
    OrthoDBi EOG600DP5.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci BCLA66692:GHMP-2719-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
      Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KSM-K16.

    Entry informationi

    Entry nameiLEUC_BACSK
    AccessioniPrimary (citable) accession number: Q5WEN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3