Reviewed, UniProtKB/Swiss-Prot Q5WEN2 (ILVC_BACSK)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	ABC2643

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226157
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5WEN2-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1B80C1C485792761	FASTA	341	37,262
	10 20 30 40 50 60 MAKVYYNGDV NEQVLEGKTV AIVGYGSQGH AHAQNLRESG VDVIIGLRPG KSWDKATEDG 70 80 90 100 110 120 FSVYSVREAS AKADIIMILS PDEHQPAIYK ESIEPELTAG KALVFAHGFN IHFNQIVPPE 130 140 150 160 170 180 HVDVFLAAPK GPGHLVRRTY TEGAGVPALI AVYQDATGQA KDIALAYAKQ IGAARAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GTSALVKAGF ETLVEAGYQP EIAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGLEYMRYSI SDTAQWGDFQ AGPRIVTDET KAAMKEILSD IQKGKFAKGW ILENKLNRPE 310 320 330 340 YNAINEAEKN HPLEVVGREL REMMPFVKAS KSKGVVANAK N

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD65178.1.
RefSeq	YP_176139.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEN2.
Genome annotation databases
GeneID	3201311.
GenomeReviews	Gene locus ABC2643 in contig AP006627_GR.
KEGG	bcl:ABC2643.
NMPDR	fig|66692.3.peg.2940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WEN2.
OMA	AHGFNIR.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2643-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACSK

Accession

Primary (citable) accession number: Q5WEN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)