Q5WEM9 (ILVD_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	ABC2646

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225368
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5WEM9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: A0D0415BEFE44D83	FASTA	554	59,478
	10 20 30 40 50 60 MRSNMIKKGI DRAPHRSLLR AAGVKEEDMD KPFIGVCNSY IDIIPGHMHL NKFAEVAKEA 70 80 90 100 110 120 IIEAGGIPFE FNTIGVDDGI AMGHIGMRYS LPSREIICDA AETVINAHWF DGVFYIPNCD 130 140 150 160 170 180 KITPGMLMAA VRTNVPAVFV SGGPMEAGRT KEGKSLSLVS VFEGVGAFSS GKMTREELLE 190 200 210 220 230 240 IEQLACPTCG SCSGMFTANS MNSLMEMLGL ALPGNGTLVA TSTERHNLIK DAAKHLINLI 250 260 270 280 290 300 EKDIRPRDIV TEETIDDAFA LDMAMGGSTN TVLHTLAIAN EAEIDYDLTR INEVAERVPY 310 320 330 340 350 360 LCKISPASDY SMDDVHKAGG VAAIMKELIE MGAVKGDRIT ITGKSLYENV AHAQITNTDV 370 380 390 400 410 420 IRTKETAYSP VGGLSILYGN LAPDGAVIKV GAVDPSIKTF TGEAIVFNSQ EEAQEQINNG 430 440 450 460 470 480 AVKEGQVVVI RYEGPKGGPG MPEMLAPTSA IQGRGLGTKV ALITDGRFSG ASRGISIGHI 490 500 510 520 530 540 SPEAAEGGPI AFVENGDMIK IDLIERTIEW EISEEELAKR REGWTEPEPK VKKGYLARYS 550 KLVTSANTGG VMKI

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65181.1.
RefSeq	YP_176142.1. NC_006582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000047724; EBBACP00000046485; EBBACG00000047715.
GeneID	3201312.
GenomeReviews	Gene locus ABC2646 in contig AP006627_GR.
KEGG	bcl:ABC2646.
NMPDR	fig|66692.3.peg.2943.
PATRIC	18924938. VBIBacCla58185_2821.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000001430.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2646-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACSK

Accession

Primary (citable) accession number: Q5WEM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program