Q5WEM4 (NTPA_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

ABC2651

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178128
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	69 – 70	2	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium or manganese By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WEM4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 03FCDFD235EF0D6B	FASTA	198	21,585
	10 20 30 40 50 60 MNELVVATKN KGKLADFQTL FTDRYIVKSL YDYPEVPEII EDGDTFHENA AKKAETLAAY 70 80 90 100 110 120 LQKLVIADDS GLLIDALGGK PGVYSARYAG EPKNDQANID KVLSELDGVP TQKRTARFFC 130 140 150 160 170 180 VIALAEPGKE TIFAEGACEG RITEKPTGSN GFGYDPIFFV PSHGQTMAEL SAGTKNQLSH 190 RARALTALKE TIEGVWPK

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65186.1.
RefSeq	YP_176147.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WEM4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000047189; EBBACP00000045950; EBBACG00000047180.
GeneID	3204761.
GenomeReviews	Gene locus ABC2651 in contig AP006627_GR.
KEGG	bcl:ABC2651.
NMPDR	fig|66692.3.peg.2974.
PATRIC	18924954. VBIBacCla58185_2827.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
GeneTree	EBGT00050000001334.
HOGENOM	HBG697237.
OMA	GEAHNDA.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2651-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BACSK

Accession

Primary (citable) accession number: Q5WEM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program