Q5WEM3 (RNPH_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	ABC2652

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus clausii

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Ribonuclease PH HAMAP-Rule MF_00564		PRO_0000139868

Sequences

	Sequence		Length	Mass (Da)
	Q5WEM3 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 0B5B8D4E754C403D	FASTA	250	27,285
	10 20 30 40 50 60 MRQDGRKQNQ LREVEIIPHF TKHAEGSVLI SVGDTKVICT ASIENRVPPF LRGQNKGWLA 70 80 90 100 110 120 AEYSMLPRAT GQRTIREAAK GKLSGRTMEI QRLIGRALRA VVDLEKLGEK TIWIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAYVALVLA VQKAFAEKTI SAWPITDFLA AISVGILPEE GAVCDLCYSE 190 200 210 220 230 240 DSQAKVDMNI VQTGSGDFVE VQGSGEEAVY TRSELNELLD LAAEGIDQLI HIQRPFLGEA 250 ASWIEEKKGT

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65187.1.
RefSeq	YP_176148.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WEM3.
SMR	Q5WEM3. Positions 1-240.
ModBase	Search...
Protein-protein interaction databases
STRING	66692.ABC2652.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD65187; BAD65187; ABC2652.
GeneID	3201525.
KEGG	bcl:ABC2652.
PATRIC	18924956. VBIBacCla58185_2828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BCLA66692:GHMP-2645-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BACSK

Accession

Primary (citable) accession number: Q5WEM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program