Reviewed, UniProtKB/Swiss-Prot Q5WEJ5 (SYFA_BACSK)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	ABC2680

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126665
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WEJ5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 15F66E8B3556E1CC	FASTA	344	38,634
	10 20 30 40 50 60 MREQLTALKH EALDKAEVAK SEQDIQAIRV AYLGKKGPIT EVLRGMGKLS AEERPIIGAL 70 80 90 100 110 120 ANEVRDEIKN KLDEKSKKLQ EAAIKQQLEQ ESIDVTLPGR PALQGAIHPL TAVVTEIEEI 130 140 150 160 170 180 FLGLGFSIGE GPEIETDYYN FEAMNLPKDH PARDMQDSFY VTEDLLLRTQ TSPVQARTME 190 200 210 220 230 240 KFAGKGPVKV VVPGKVFRRD DDDATHSHQF MQVEGLYVDK KVRMSDLKGV LETFAKQFFG 250 260 270 280 290 300 QDRSIRLRPS FFPFTEPSVE VDVSCGICSG KGCRVCKQSG WIEVLGAGMV HPRVLEMSGF 310 320 330 340 DANEYSGFAF GMGVERLAML KYDIDDIRQF YANDLRFLMQ FKDV

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD65215.1.
RefSeq	YP_176176.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEJ5.
Genome annotation databases
GeneID	3202105.
GenomeReviews	Gene locus ABC2680 in contig AP006627_GR.
KEGG	bcl:ABC2680.
NMPDR	fig|66692.3.peg.3002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WEJ5.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2680-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BACSK

Accession

Primary (citable) accession number: Q5WEJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)