Q5WEG2 (MDH_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	ABC2713

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus clausii

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113433
Regions
	Nucleotide binding	12 – 17	6	NAD By similarity
	Nucleotide binding	123 – 125	3	NAD By similarity
Sites
	Active site	180	1	Proton acceptor By similarity
	Binding site	36	1	NAD By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	100	1	NAD By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
Amino acid modifications
	Modified residue	149	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WEG2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: ED99F51CA5F3F8D1	FASTA	314	33,597
	10 20 30 40 50 60 MAIKRRKISV IGSGFTGATT ALMVAQKELG DVVLLDIPNM EGPTKGKALD MLESTPVQGV 70 80 90 100 110 120 DSTITGTSSY EDTKDSDVVV ITAGIARKPG MSRDDLVATN AKIMKSVTKE VVKYSPNSYI 130 140 150 160 170 180 IVLTNPADAM TYTVYKESGF PKNRVIGQSG VLDTARFRTF VAQELNVSVE DVTGFVLGGH 190 200 210 220 230 240 GDDMVPLIRY SAAGGVPLTK LIAPERIEEI VERTRKGGGE IVGLLGNGSA YYAPAASLTQ 250 260 270 280 290 300 MVEAILKDKK RIIPTIAYLE GEYGQHDLYL GVPTILGGDG IEKVIELDLT EEEKAQLDKS 310 VQSVRNVMAA LPAE

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65248.1.
RefSeq	YP_176209.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WEG2.
SMR	Q5WEG2. Positions 5-310.
ModBase	Search...
Protein-protein interaction databases
STRING	66692.ABC2713.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD65248; BAD65248; ABC2713.
GeneID	3204432.
KEGG	bcl:ABC2713.
PATRIC	18925084. VBIBacCla58185_2892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	BCLA66692:GHMP-2706-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BACSK

Accession

Primary (citable) accession number: Q5WEG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program