Reviewed, UniProtKB/Swiss-Prot Q5WEF6 (K6PF_BACSK)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	ABC2719

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	6-phosphofructokinase HAMAP MF_00339		PRO_1000059746
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WEF6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 45D26DB43AE2C5A0	FASTA	319	34,405
	10 20 30 40 50 60 MKRIGVLTSG GDSPGMNAAI RAVVRKAIFH GLEVYGISYG YQGLINGDIK KMELGSVGDI 70 80 90 100 110 120 IHRGGTMLYT ARCEEFKTLE GQQKGIEQLK KFGIEGLVVI GGDGSYRGAQ QLTKHGFPTI 130 140 150 160 170 180 GVPGTIDNDI PGTDFTIGFD TALNTVIDAI DKIRDTATSH DRTYVIEVMG RHAGDLALWA 190 200 210 220 230 240 GLADGAETIV IPEAEHNMEQ ILATIKRGQE RGKKHSIIVV AEGVGSGIEF GDRISKEMNM 250 260 270 280 290 300 ETRVTVLGHI QRGGSPTGAD RVLASRLGAK AVDLLLEGKA GMTVGIQKNE LVYHPIDEIL 310 DRPHTIDQEM YKLSQELSI

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD65254.1.
RefSeq	YP_176215.1.
3D structure databases
SMR	Q5WEF6. Positions 1-319.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEF6.
Genome annotation databases
GeneID	3202098.
GenomeReviews	Gene locus ABC2719 in contig AP006627_GR.
KEGG	bcl:ABC2719.
NMPDR	fig|66692.3.peg.3060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WEF6.
OMA	DTCLNTV.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2719-MON.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_BACSK

Accession

Primary (citable) accession number: Q5WEF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)