Reviewed, UniProtKB/Swiss-Prot Q5WED8 (ASSY_BACSK)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	ABC2737

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148571
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity
Sites
	Binding site	86	1	Citrulline By similarity
	Binding site	116	1	ATP; via amide nitrogen By similarity
	Binding site	118	1	Aspartate By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	122	1	Citrulline By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	174	1	Citrulline By similarity
	Binding site	183	1	Citrulline By similarity
	Binding site	259	1	Citrulline By similarity
	Binding site	271	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WED8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 21B3467205BA8631	FASTA	403	44,510
	10 20 30 40 50 60 MAKEKVVLAY SGGLDTSVAV KWLTDKGYDV IAVGLDVGEG KDLEFVKQKA LQVGAIQSYT 70 80 90 100 110 120 IDAKKEYAES FVLPALQAHA LYEQKYPLVS ALSRPLISKK LVEIAEQTGA TAVAHGCTGK 130 140 150 160 170 180 GNDQVRFEVS IQALNPNLKV LAPVREWAWS RDEEIEYAKA HNIPIPIDLD NPYSVDQNLW 190 200 210 220 230 240 GRSNECGVLE DPWATPPEGA YALTAPLEKT PDTPDIIELS FEKGVPVAIN GEAYPLDQLI 250 260 270 280 290 300 LTLNEIAGKH GVGRIDHVEN RLVGIKSREV YECPGAMTLI KAHKELEDLT LPKELAHFKP 310 320 330 340 350 360 VIEKKLTELI YEGLWFSSLQ PALLAFLKES QTHVTGVVRV KLFKGHAIIE GRKSAYSLYN 370 380 390 400 EKLATYTPDD EFDHSAAVGF ISLWGLPTKV HSMVTKEKKE VTL

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD65272.1.
RefSeq	YP_176233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WED8.
Genome annotation databases
GeneID	3201717.
GenomeReviews	Gene locus ABC2737 in contig AP006627_GR.
KEGG	bcl:ABC2737.
NMPDR	fig|66692.3.peg.2593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WED8.
OMA	IAPVREW.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2737-MON.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BACSK

Accession

Primary (citable) accession number: Q5WED8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)