Q5WEB8 (SYY_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	ABC2758

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Tyrosine--tRNA ligase HAMAP MF_02007		PRO_0000236692
Regions
	Domain	352 – 413	62	S4 RNA-binding
	Motif	57 – 66	10	"HIGH" region HAMAP MF_02007
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WEB8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 384E26C03B8BA7F1	FASTA	414	47,012
	10 20 30 40 50 60 MDEKQPLTPE QQKLVDEQVE ALMRGVVEVV PKEAFREKIE KSVRTGKPLN IKLGMDPSAP 70 80 90 100 110 120 DVHIGHTVVL QKLRQFQEYG HHIQLLIGDF TGKIGDPTGK SETRKVLTDE QVKQNAQTYV 130 140 150 160 170 180 EQYGKILDIE KTEILYNSRW LSELKFDDVL KLAGQMTVAR MLEREDFSKR YKTGQPISVH 190 200 210 220 230 240 EFFYPLMQGY DSVAMETDIE VGGTDQTFNL LMGRQLQEAY GKEKQVMLTL PLIEGLDGVR 250 260 270 280 290 300 KMSKSLNNYI GIDEAPNEIF GKAMSIPDEL MVKYYKLATD VPMDEIEALE KGLADGSVHP 310 320 330 340 350 360 RDAKMRLGHK FVEMYHGKEA ADEAEQYFKT VFQKRALPED IPVFSWEGDK EVPLIDLLVT 370 380 390 400 410 LNMQSSKGEA RRMIQGGGVK INEQKITDIH TVVSVEDNMI VQVGKRKFAK LSLT

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65292.1.
RefSeq	YP_176253.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WEB8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000049335; EBBACP00000048096; EBBACG00000049326.
GeneID	3203575.
GenomeReviews	Gene locus ABC2758 in contig AP006627_GR.
KEGG	bcl:ABC2758.
NMPDR	fig|66692.3.peg.2647.
PATRIC	18925174. VBIBacCla58185_2937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
GeneTree	EBGT00050000000599.
HOGENOM	HBG288125.
OMA	YVVQVGK.
ProtClustDB	PRK13354.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2758-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BACSK

Accession

Primary (citable) accession number: Q5WEB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program