Q5WEA8 (MURC_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	ABC2768

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182054
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5WEA8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 71D03ABAAB1655BE	FASTA	435	48,203
	10 20 30 40 50 60 MTNYHFTGIK GSGMSALAQI LHDLNENVQG SDIEETIFTQ KPLERKGITL LPFSRDNIKN 70 80 90 100 110 120 GQHVIVSAAY GETHEEIKRA RELGLKVDVY PEFLGSFIKQ FTSIAVSGSH GKTSTTGLLA 130 140 150 160 170 180 HVLGSVEPTS YLIGDGTGKG AANSTYFVFE ACEYRRHFLN YSPDYCVMTN IDFDHPDYFK 190 200 210 220 230 240 SVEDVVSAFQ TMAKQVNKAI IACGDDEHLQ TLQANVPIVY YGLGDHNDFQ AKSIKNTPEG 250 260 270 280 290 300 TAFDAWVRGD LFGSFVIPGF GDHNVKNALS VIALCHYEGI GYEDIVEHLK TFAGVKRRFS 310 320 330 340 350 360 EKRSGKQILI DDYAHHPTEI AATIEATKKK YHDREVTAIF QPHTFTRTKT FLNEFAEALS 370 380 390 400 410 420 QADHVYLCDI FGSAREEQGS LSIEQLQALV DGAKLLTEAE IETLKGHGES VLLFMGAGDI 430 QKYQKAYEAL IESSS

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65302.1.
RefSeq	YP_176263.1. NC_006582.1.
3D structure databases
ProteinModelPortal	Q5WEA8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WEA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000047310; EBBACP00000046071; EBBACG00000047301.
GeneID	3204494.
GenomeReviews	Gene locus ABC2768 in contig AP006627_GR.
KEGG	bcl:ABC2768.
NMPDR	fig|66692.3.peg.2657.
PATRIC	18925194. VBIBacCla58185_2947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
GeneTree	EBGT00050000000630.
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2768-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACSK

Accession

Primary (citable) accession number: Q5WEA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program