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Q5WE05 (SYL_BACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ABC2871
OrganismBacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]
Taxonomic identifier66692 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151972

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif574 – 5785"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5771ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5WE05 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: D97D7DA775C5AB79

FASTA80491,100
        10         20         30         40         50         60 
MSFSHQEIEK KWQAFWEENK TFKTDEQAEG PHFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGKHPATFT KQNIDTFKRQ IKELGFSYDW 

       130        140        150        160        170        180 
DREISTTDPH YYKWTQWIFL KLYEKGLAYI DEVAVNWCPA LGTVLANEEI VDGVSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW VLRITAYAER LLEDLEELEW PESLKDMQRN WIGKSEGAEV TFKINEHSVN 

       250        260        270        280        290        300 
VFTTRPDTLF GATYMVLAPE HKLVTEITTD EQKEAVEAYQ KQVALKSDIE RTDLAKEKTG 

       310        320        330        340        350        360 
AFTGAYAINP VNGEKIPVWI ADYVLISYGT GAVMAVPAHD ERDFEFANAF GLPIKEVVAG 

       370        380        390        400        410        420 
GDVSKAAYTG DGEHVNSDFL NGLNKQEAVE KMIVWLEENG AGQRKVTYRL RDWLFSRQRY 

       430        440        450        460        470        480 
WGEPIPIIHW EDGSMSALDE SELPLVLPDL EEIKPSGTGE SPLANAKDWL EVVDPKTGMR 

       490        500        510        520        530        540 
GRRETNTMPQ WAGSCWYYLR YIDPTNDEAL ADPEKLKNWL PVDTYIGGAE HAVLHLLYAR 

       550        560        570        580        590        600 
FWHKFLYDIG VVPTKEPFQK VFNQGMILGE NNEKMSKSKG NVVNPDEIIA SHGADTLRLY 

       610        620        630        640        650        660 
EMFMGPLDAS VAWSTNGLDG SRRFLERVWR LIVNEETGKL NSNVKDVEGN EAFVRTYHQT 

       670        680        690        700        710        720 
VKKVTEDFAE LRFNTGISQL MVFVNEGNKQ EVLPKALIEG FVKLLSPVAP HIAEELWEKL 

       730        740        750        760        770        780 
GHTDTITYEA WPTYDESLLV ENEVEVVVQM NGKVKTKLVI NKGASKEEME AAALADEKVQ 

       790        800 
AAIGEKTIRK VIAVPGKLVN IVVG 

« Hide

References

[1]"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KSM-K16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006627 Genomic DNA. Translation: BAD65405.1.
RefSeqYP_176366.1. NC_006582.1.

3D structure databases

ProteinModelPortalQ5WE05.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING66692.ABC2871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD65405; BAD65405; ABC2871.
GeneID3204060.
KEGGbcl:ABC2871.
PATRIC18925428. VBIBacCla58185_3064.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBCLA66692:GHMP-2952-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACSK
AccessionPrimary (citable) accession number: Q5WE05
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries