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Q5WDW9 (DDL_BACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:ABC2907
OrganismBacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]
Taxonomic identifier66692 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000057321

Regions

Domain145 – 348204ATP-grasp
Nucleotide binding175 – 23056ATP By similarity

Sites

Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 2 By similarity
Metal binding3171Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5WDW9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 081C342A37B1F811

FASTA36840,081
        10         20         30         40         50         60 
MAKKRVGIIF GGKSAEHEVS LQSAKNIVEA IDKDRFDVTL IGIDKQGQWH INEASNFLLN 

        70         80         90        100        110        120 
ADNPALIELN KSNQGVALIP GKEDRQLVGT ANQSVLDQLD VVFPIVHGTL GEDGSLQGMF 

       130        140        150        160        170        180 
RLANLPFVGS NVLGSAVSMD KDIAKRLLQG AGLHVASGLT FTRAAKETID FESIADQLGL 

       190        200        210        220        230        240 
PLFIKPANQG SSVGVNKATT EAEFTAAIEE AFSYDHKVLI EAAIKGREIE CAVLGNDYPK 

       250        260        270        280        290        300 
ASTCGEILPQ DGFYSYDAKY IDEDGAKLAI PADLPEDVNR RIQDIAIQAY KTLNCEGLAR 

       310        320        330        340        350        360 
VDVFLTETGE VIINEVNTLP GFTKISMYPK LWEASGVSYQ DLITTLIELA IERHERDKQL 


KSSVFDRN 

« Hide

References

[1]"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KSM-K16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006627 Genomic DNA. Translation: BAD65441.1.
RefSeqYP_176402.1. NC_006582.1.

3D structure databases

ProteinModelPortalQ5WDW9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING66692.ABC2907.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD65441; BAD65441; ABC2907.
GeneID3203892.
KEGGbcl:ABC2907.
PATRIC18925508. VBIBacCla58185_3103.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycBCLA66692:GHMP-2989-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BACSK
AccessionPrimary (citable) accession number: Q5WDW9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways