Reviewed, UniProtKB/Swiss-Prot Q5WDT6 (KHSE_BACSK)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	ABC2940

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_0000156553
Regions
	Nucleotide binding	84 – 94	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5WDT6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 9546BD9B1A66C60B	FASTA	297	31,324
	10 20 30 40 50 60 MAFSITVPAS TANLGPGFDS IGLALNRYLH LEVEQASKWS FICSSPGLEN IGTDNLVTKA 70 80 90 100 110 120 ATFASKEWGK VLPPCQVVMK NDIPLSKGFG SSAAAIVAGI ELAASVCGQF ASKAEKARLA 130 140 150 160 170 180 SLWEGHPDNV AASIYGGLVI GTHSEESTEI LHVEAPNIDL VALIPSKTLA TKKARAVLPD 190 200 210 220 230 240 MLSYKEAVQA SSVANVLAAA LVKQDWELVG KMMLADRFHQ PYRQQLIPHL PDVCAYAQAD 250 260 270 280 290 EDAYGAALSG AGPIILCLVK EGKGEAFASR LHKQFPACRA EVMRPAVQGS AVSIAQA

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65474.1.
RefSeq	YP_176435.1.
3D structure databases
SMR	Q5WDT6. Positions 1-276.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WDT6.
Genome annotation databases
GeneID	3203418.
GenomeReviews	Gene locus ABC2940 in contig AP006627_GR.
KEGG	bcl:ABC2940.
NMPDR	fig|66692.3.peg.2828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HBG646290.
OMA	FDCLGAA.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2940-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR000870. Homoserine_kin. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACSK

Accession

Primary (citable) accession number: Q5WDT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)