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Q5WDS6

- LIPA_BACSK

UniProt

Q5WDS6 - LIPA_BACSK

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Protein

Lipoyl synthase

Gene
lipA, ABC2950
Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi70 – 701Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-3032-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:ABC2950
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001168: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Lipoyl synthaseUniRule annotationPRO_0000325231Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi66692.ABC2950.

Structurei

3D structure databases

ProteinModelPortaliQ5WDS6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5WDS6-1 [UniParc]FASTAAdd to Basket

« Hide

MPEKDEKEQH MRKPDWLKIK LNTNESYTGL KKMMREKKLH TVCEEARCPN    50
IHECWAVRKT ATFMILGDIC TRGCRFCAVK TGLPTELDLQ EPERVAESVE 100
TMGLKHVVIT AVARDDLKDG GAAVFAETVR AVRRKNPFCT IEVLPSDMLG 150
NESSLQTLMD ARPNIMNHNI ETVRRLTPKV RARAKYDRTL EFLRRAKEMH 200
PDIPTKSSLM VGLGETKEEI LETMDDLRAN NVDILTIGQY LQPTKKHLKV 250
IKYYHPDEFA ELKEIAMQKG FSHCEAGPLV RSSYHADEQV NQAQVNMEAR 300
KAQGEQQRV 309
Length:309
Mass (Da):35,375
Last modified:November 23, 2004 - v1
Checksum:i6A8F95F584FAFEE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD65484.1.
RefSeqiYP_176445.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD65484; BAD65484; ABC2950.
GeneIDi3204075.
KEGGibcl:ABC2950.
PATRICi18925598. VBIBacCla58185_3148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006627 Genomic DNA. Translation: BAD65484.1 .
RefSeqi YP_176445.1. NC_006582.1.

3D structure databases

ProteinModelPortali Q5WDS6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 66692.ABC2950.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD65484 ; BAD65484 ; ABC2950 .
GeneIDi 3204075.
KEGGi bcl:ABC2950.
PATRICi 18925598. VBIBacCla58185_3148.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

BioCyci BCLA66692:GHMP-3032-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
    Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KSM-K16.

Entry informationi

Entry nameiLIPA_BACSK
AccessioniPrimary (citable) accession number: Q5WDS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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