Reviewed, UniProtKB/Swiss-Prot Q5WDS1 (TYSY_BACSK)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	ABC2955

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140930
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WDS1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E32BE7272D2EAAA8	FASTA	264	30,508
	10 20 30 40 50 60 MEQYLQLCEH VLQNGSPKSD RTGTGTISVF GYQMRFDLEK GFPIVTTKKL HMRSIIHELL 70 80 90 100 110 120 WFLKGDTNIR YLQENGVRIW NEWADENGDL GPVYGKQWRS WEGANGKTVD QITEVVEQIK 130 140 150 160 170 180 TNPNSRRLIV NAWNVAEIEE MALAPCHCLF QFYVNDGKLS CQLYQRSADI FLGVPFNISS 190 200 210 220 230 240 YALLTMMMAQ VCGLKPGEFI HTFGDAHLYN NHIEQTKLQL TRKPKQLPTM HINPEVTDLF 250 260 AFTYDDFELK HYDPYPHIKA EVSV

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65489.1.
RefSeq	YP_176450.1.
3D structure databases
SMR	Q5WDS1. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WDS1.
Genome annotation databases
GeneID	3203422.
GenomeReviews	Gene locus ABC2955 in contig AP006627_GR.
KEGG	bcl:ABC2955.
NMPDR	fig|66692.3.peg.2844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC2955-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACSK

Accession

Primary (citable) accession number: Q5WDS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)