Reviewed, UniProtKB/Swiss-Prot Q5WD10 (GUAC_BACSK)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	ABC3216

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	GMP reductase HAMAP MF_01511		PRO_0000093750
Regions
	Nucleotide binding	205 – 228	24	NADP Potential
Sites
	Active site	176	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WD10-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 2B8E4B033DBDA8FF	FASTA	328	36,286
	10 20 30 40 50 60 MMENVFDYED IQLIPAKCIV GSRAECDTSV ELGGRTFKLP VVPANMQTII DENIARYLAE 70 80 90 100 110 120 NDYFYIMHRF QPETRLAFVK DMHERGLYAS ISVGVKEEEY TFVQQLADQH VVPEYVTIDI 130 140 150 160 170 180 AHGHSEAVIN MIRHIKTHLP DSFVIAGNVG TPEAVRELEH AGADATKVGI GPGKVCITKI 190 200 210 220 230 240 KTGFGTGGWQ LAALRWCAKA ASKPIIADGG IRTHGDIAKS VRFGATMVMI GSLFAGHEES 250 260 270 280 290 300 PGDTIEKDGK LYKEYFGSAS EYQKGERKNV EGKKMYVEHK GSLAETLLEM EQDLQSSISY 310 320 AGGKRLDAIR NVDYVVVKNS IFNGDKIY

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD65750.1.
RefSeq	YP_176711.1.
3D structure databases
SMR	Q5WD10. Positions 2-322.
ModBase	Search...
Genome annotation databases
GeneID	3204556.
GenomeReviews	Gene locus ABC3216 in contig AP006627_GR.
KEGG	bcl:ABC3216.
NMPDR	fig|66692.3.peg.3197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WD10.
OMA	Q5WD10. NSRSECD.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC3216-MON.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACSK

Accession

Primary (citable) accession number: Q5WD10

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)