Q5WCP8 (HUTI_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	ABC3329

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Imidazolonepropionase HAMAP MF_00372		PRO_0000306432
Sites
	Metal binding	85	1	Zinc or iron By similarity
	Metal binding	87	1	Zinc or iron By similarity
	Metal binding	255	1	Zinc or iron By similarity
	Metal binding	329	1	Zinc or iron By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WCP8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F54C70EE28632E3E	FASTA	447	47,914
	10 20 30 40 50 60 MAELLYLKEA EQVVTVAGAS DKPKVGEDLS EIGVIERGSV IVEGEKIAFA GTDADARHYL 70 80 90 100 110 120 QKRSGKVKTI EATGKILTPG LVDPHTHLVF AGSREQELTM RLKGKSYMSI LQAGGGILST 130 140 150 160 170 180 TKSTRAATAD QLAHESRLRL DRFLQHGVTT VEAKSGYGLA TDAELKQLRV AQQLNNDHPV 190 200 210 220 230 240 DVVSTFMGAH AIPPEWKQDP NGFVQLVAEE MIPQVAAENL AEFCDVFCEE GVFTVAQSQY 250 260 270 280 290 300 ILEEGKKHGL KPKIHADELV SFGGAELAAK VGAVSADHLL KASPTGIEQM AEAGVIAVLL 310 320 330 340 350 360 PGTAFFLMTE PANARAMIEA GVPVALSTDR NPGSSPTESL PFIMNLACLT MKMTPEEVLA 370 380 390 400 410 420 ASTINAAHAI GRAKDIGSIE AGKNADLVLF DAPNYQTLQY NYAVNRVDTV MKAGKIVVEG 430 440 GVLLEKTDNQ RCSLATEDRG TRPIWQK

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD65862.1.
RefSeq	YP_176823.1. NC_006582.1.
3D structure databases
HSSP	HSSP built from PDB template 2GOK based on UniProtKB Q8U8Z6.
ProteinModelPortal	Q5WCP8.
SMR	Q5WCP8. Positions 10-419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WCP8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000045998; EBBACP00000044759; EBBACG00000045989.
GeneID	3201303.
GenomeReviews	Gene locus ABC3329 in contig AP006627_GR.
KEGG	bcl:ABC3329.
NMPDR	fig|66692.3.peg.3344.
PATRIC	18926390. VBIBacCla58185_3543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
GeneTree	EBGT00050000002251.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC3329-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BACSK

Accession

Primary (citable) accession number: Q5WCP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program