Q5WBJ6 (ALLB_BACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allantoinase
EC=3.5.2.5

Alternative name(s):
Allantoin-utilizing enzyme

Gene names

Name:	allB
Synonyms:	pucH
Ordered Locus Names:	ABC3732

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus clausii

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring By similarity. HAMAP-Rule MF_01645
Catalytic activity	(S)-allantoin + H2O = allantoate. HAMAP-Rule MF_01645
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1. HAMAP-Rule MF_01645
Subunit structure	Homotetramer By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity. HAMAP-Rule MF_01645
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	allantoin catabolic process Inferred from electronic annotation. Source: HAMAP purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	allantoinase activity Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Allantoinase HAMAP-Rule MF_01645		PRO_0000317669
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	146	1	Zinc 1; via carbamate group By similarity
	Metal binding	146	1	Zinc 2; via carbamate group By similarity
	Metal binding	185	1	Zinc 2 By similarity
	Metal binding	239	1	Zinc 2 By similarity
	Metal binding	312	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	146	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WBJ6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 903BD489E7C7ACF5	FASTA	448	48,711
	10 20 30 40 50 60 MLDLCITGGR VVLPSGVEET DVGIQAGKIA RIGPINKKEA RCIMNANGQY VFPGAVDTHV 70 80 90 100 110 120 HFSEPGRTEW EGFFTGSRSL AAGGTTTYVE MPLNALPATT NRANLQRKLE AAKGQNYVDY 130 140 150 160 170 180 SFYGGLVPTN LHELADLSAS GVVAFKCFLS PCGSDIPGDF RNVDLNGLRA GMRLLAEKGQ 190 200 210 220 230 240 LLCVHAEDPS MISQLEAKLL SPVGADAYVA SRPVEAEVKA VCDTLAAARE TGCRIHFVHI 250 260 270 280 290 300 SSAAAIEAIE RAKEEGVDVT VESCPHYFLL SAEELAELGP LAKCQPPLRP KQEQAKLWAC 310 320 330 340 350 360 LLDGQIDWLA SDHSPCTPDL KDGDFLTAWG GISGCQNNID IMFDAAVKRR GMPPEQLARL 370 380 390 400 410 420 IATNPAKRMN LREKGEIAIG KDADFAFVDD RQSYTLTKEQ LYYKNKHSPY VGRTIGCKVR 430 440 RVLLRGQTIY TEEKGIIGKP SGELLHIH

References

[1]

"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KSM-K16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006627 Genomic DNA. Translation: BAD66264.1.
RefSeq	YP_177225.1. NC_006582.1.
3D structure databases
HSSP	HSSP built from PDB template 1XRT based on UniProtKB O66990.
ProteinModelPortal	Q5WBJ6.
ModBase	Search...
Protein-protein interaction databases
STRING	66692.ABC3732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD66264; BAD66264; ABC3732.
GeneID	3202800.
KEGG	bcl:ABC3732.
PATRIC	18927260. VBIBacCla58185_3978.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0044.
HOGENOM	HOG000219146.
KO	K01466.
OMA	CRLHICH.
Enzyme and pathway databases
BioCyc	BCLA66692:GHMP-3722-MONOMER.
UniPathway	UPA00395; UER00653.
Family and domain databases
HAMAP	MF_01645. Hydantoinase.
InterPro	IPR017593. Allantoinase. IPR013108. Amidohydro_3. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
Pfam	PF07969. Amidohydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR03178. allantoinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALLB_BACSK

Accession

Primary (citable) accession number: Q5WBJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program