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Protein

Allantoinase

Gene

allB

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring.UniRule annotation

Catalytic activityi

(S)-allantoin + H2O = allantoate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathway: (S)-allantoin degradation

This protein is involved in step 1 of the subpathway that synthesizes allantoate from (S)-allantoin.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Allantoinase (allB)
This subpathway is part of the pathway (S)-allantoin degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes allantoate from (S)-allantoin, the pathway (S)-allantoin degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Zinc 1UniRule annotation
Metal bindingi61 – 611Zinc 1UniRule annotation
Metal bindingi146 – 1461Zinc 1; via carbamate groupUniRule annotation
Metal bindingi146 – 1461Zinc 2; via carbamate groupUniRule annotation
Metal bindingi185 – 1851Zinc 2UniRule annotation
Metal bindingi239 – 2391Zinc 2UniRule annotation
Metal bindingi312 – 3121Zinc 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-3813-MONOMER.
UniPathwayiUPA00395; UER00653.

Names & Taxonomyi

Protein namesi
Recommended name:
AllantoinaseUniRule annotation (EC:3.5.2.5UniRule annotation)
Alternative name(s):
Allantoin-utilizing enzymeUniRule annotation
Gene namesi
Name:allBUniRule annotation
Synonyms:pucHUniRule annotation
Ordered Locus Names:ABC3732
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001168 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448AllantoinasePRO_0000317669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.UniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi66692.ABC3732.

Structurei

3D structure databases

ProteinModelPortaliQ5WBJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Allantoinase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0044.
HOGENOMiHOG000219146.
KOiK01466.
OMAiHAEMIPP.
OrthoDBiEOG6KHFW6.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01645. Hydantoinase.
InterProiIPR017593. Allantoinase.
IPR013108. Amidohydro_3.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF07969. Amidohydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR03178. allantoinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5WBJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDLCITGGR VVLPSGVEET DVGIQAGKIA RIGPINKKEA RCIMNANGQY
60 70 80 90 100
VFPGAVDTHV HFSEPGRTEW EGFFTGSRSL AAGGTTTYVE MPLNALPATT
110 120 130 140 150
NRANLQRKLE AAKGQNYVDY SFYGGLVPTN LHELADLSAS GVVAFKCFLS
160 170 180 190 200
PCGSDIPGDF RNVDLNGLRA GMRLLAEKGQ LLCVHAEDPS MISQLEAKLL
210 220 230 240 250
SPVGADAYVA SRPVEAEVKA VCDTLAAARE TGCRIHFVHI SSAAAIEAIE
260 270 280 290 300
RAKEEGVDVT VESCPHYFLL SAEELAELGP LAKCQPPLRP KQEQAKLWAC
310 320 330 340 350
LLDGQIDWLA SDHSPCTPDL KDGDFLTAWG GISGCQNNID IMFDAAVKRR
360 370 380 390 400
GMPPEQLARL IATNPAKRMN LREKGEIAIG KDADFAFVDD RQSYTLTKEQ
410 420 430 440
LYYKNKHSPY VGRTIGCKVR RVLLRGQTIY TEEKGIIGKP SGELLHIH
Length:448
Mass (Da):48,711
Last modified:November 23, 2004 - v1
Checksum:i903BD489E7C7ACF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD66264.1.
RefSeqiWP_011248568.1. NC_006582.1.
YP_177225.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD66264; BAD66264; ABC3732.
KEGGibcl:ABC3732.
PATRICi18927260. VBIBacCla58185_3978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD66264.1.
RefSeqiWP_011248568.1. NC_006582.1.
YP_177225.1. NC_006582.1.

3D structure databases

ProteinModelPortaliQ5WBJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi66692.ABC3732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD66264; BAD66264; ABC3732.
KEGGibcl:ABC3732.
PATRICi18927260. VBIBacCla58185_3978.

Phylogenomic databases

eggNOGiCOG0044.
HOGENOMiHOG000219146.
KOiK01466.
OMAiHAEMIPP.
OrthoDBiEOG6KHFW6.

Enzyme and pathway databases

UniPathwayiUPA00395; UER00653.
BioCyciBCLA66692:GHMP-3813-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01645. Hydantoinase.
InterProiIPR017593. Allantoinase.
IPR013108. Amidohydro_3.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF07969. Amidohydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR03178. allantoinase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
    Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KSM-K16.

Entry informationi

Entry nameiALLB_BACSK
AccessioniPrimary (citable) accession number: Q5WBJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 23, 2004
Last modified: May 27, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.