Reviewed, UniProtKB/Swiss-Prot Q5WB48 (MURA2_BACSK)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murZ
Ordered Locus Names:	ABC3881

Organism

Bacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]

Taxonomic identifier

66692 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231160
Sites
	Active site	116	1	Proton donor By similarity
	Binding site	116	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5WB48-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: FDAE3BDC2B65CEDA	FASTA	428	45,956
	10 20 30 40 50 60 MQKLLIEGGH TLEGTVSISG AKNSAVALIP AAILADSAVV IDNLPVISDV DLLAELLREI 70 80 90 100 110 120 GGDVDLGNQT MTIHPEKMVA MPLPNGRVKK LRASYYLMGA MLGKFKKAVI GLPGGCNLGP 130 140 150 160 170 180 RPIDQHIKGF EALGAQVTNE QGAIYLRANE LRGAKIYLDV VSVGATINIM LAAVRAKGRT 190 200 210 220 230 240 IIENAAKEPE IIDVATLLTS MGAKIKGAGT NVIRIDGVDH LQGCRHSIIP DRIEAGTYMI 250 260 270 280 290 300 MAAAMGRRVH IDNVIPTHLE SVTAKLREMG TTVEEYDDQL IISGMDPKKG VDIKTLVYPG 310 320 330 340 350 360 FPTDLQQPFT SLLTSAEGTS IVTDTIYDAR FKHVDELRRM GASVKVEGRS SIVNGKSSLQ 370 380 390 400 410 420 GAKVKASDLR AGAALVIAGL MAEGITEVSG LEHIDRGYEH LETKLTRLGA KVWREELTEE ELEQVKQS

References

[1]	"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16." Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006627 Genomic DNA. Translation: BAD66412.1.
RefSeq	YP_177373.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5WB48.
Genome annotation databases
GeneID	3204499.
GenomeReviews	Gene locus ABC3881 in contig AP006627_GR.
KEGG	bcl:ABC3881.
NMPDR	fig|66692.3.peg.3856.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5WB48.
OMA	DNVIPTH.
Enzyme and pathway databases
BioCyc	BCLA66692:ABC3881-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_BACSK

Accession

Primary (citable) accession number: Q5WB48

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)