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Q5WAX6 (TOP3_BACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:ABC3953
OrganismBacillus clausii (strain KSM-K16) [Complete proteome] [HAMAP]
Taxonomic identifier66692 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   LigandDNA-binding
Magnesium
Metal-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286365

Regions

Domain3 – 136134Toprim
Region187 – 1926Interaction with DNA By similarity

Sites

Active site3111O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium; catalytic By similarity
Metal binding1051Magnesium; catalytic By similarity
Site611Interaction with DNA By similarity
Site1681Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site3131Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5WAX6 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 538DCA474A448178

FASTA72982,115
        10         20         30         40         50         60 
MKKKAVLAEK PSVARDIARV LGCNQKGNGY LEGNGYIITW ALGHLVTHAD PEHYGKQYKT 

        70         80         90        100        110        120 
WKLEELPMLP KRLELVVIKQ TAKQFHAVKH VLNRQDVGEV VIATDAGREG ELVARWILEK 

       130        140        150        160        170        180 
AHVKKPVKRL WISSVTDKAI KDGFKQLKDG KRYETLYAAA AARAEADWYV GINGTRALTT 

       190        200        210        220        230        240 
KHNAQLSCGR VQTPTLAILA DREEAIRKFV PKPYHTISVE TKDGVTFRWA DRRSKEERIF 

       250        260        270        280        290        300 
DDKRAKTLQE KLQQSAIVID SVNAKTKRTP PPPLYDLTEL QREANKRFGY SAKETLAALQ 

       310        320        330        340        350        360 
RLYEQHKAVT YPRTDSRYLS SDLVETLKER LHAVDVQPFR KMVANAKKQG LANAKRQMVN 

       370        380        390        400        410        420 
DAKVSDHHAL IPTEQPQSAS AMSDKEAKLY HLIVRRFLAN FFPPSESELT SVEATANGEL 

       430        440        450        460        470        480 
LRAKGERLVS AGWRGQETGE EDEALARLLP AMSKGDKKAV KWVGMTQGKT SPPPRFNEGT 

       490        500        510        520        530        540 
LLAAMEKPAQ HMEAKDETIK KTLAQAGGIG TVATRADIIE KLFSSFYIEK KGKDLYITSK 

       550        560        570        580        590        600 
GKQLLELVPA ELKSPALTAE WEQRLEKIVQ GKEQKQMFIA EMKEYAKAVV AQVKADTATF 

       610        620        630        640        650        660 
RHDNKTGEKC PECGKFLLEV NGKKGKMRVC QDRECGYRKN IAMTTNARCP KCKKKLELRG 

       670        680        690        700        710        720 
EGEGQVFACV CGHREKKAQF EQRRKQNKHK NVSKREVQSY MKKQNKQDQF ANSALADQLA 


KLGLKGDDS 

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References

[1]"The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KSM-K16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006627 Genomic DNA. Translation: BAD66484.1.
RefSeqYP_177445.1. NC_006582.1.

3D structure databases

ProteinModelPortalQ5WAX6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING66692.ABC3953.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD66484; BAD66484; ABC3953.
GeneID3203429.
KEGGbcl:ABC3953.
PATRIC18927718. VBIBacCla58185_4207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.

Enzyme and pathway databases

BioCycBCLA66692:GHMP-4033-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_BACSK
AccessionPrimary (citable) accession number: Q5WAX6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 23, 2004
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families