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Protein

DNA topoisomerase 3

Gene

topB

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium; catalyticUniRule annotation1
Metal bindingi105Magnesium; catalyticUniRule annotation1
Active sitei311O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciBCLA66692:G1G25-4145-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:ABC3953
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002863651 – 729DNA topoisomerase 3Add BLAST729

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei168Interaction with DNAUniRule annotation1
Sitei176Interaction with DNAUniRule annotation1
Sitei313Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi66692.ABC3953

Structurei

3D structure databases

ProteinModelPortaliQ5WAX6
SMRiQ5WAX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 136ToprimUniRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 192Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9U Bacteria
COG0550 LUCA
COG0551 LUCA
HOGENOMiHOG000086847
KOiK03169
OMAiTYPRVDT
OrthoDBiPOG091H028X

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.101 hit
1.10.460.102 hits
2.70.20.102 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q5WAX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKAVLAEK PSVARDIARV LGCNQKGNGY LEGNGYIITW ALGHLVTHAD
60 70 80 90 100
PEHYGKQYKT WKLEELPMLP KRLELVVIKQ TAKQFHAVKH VLNRQDVGEV
110 120 130 140 150
VIATDAGREG ELVARWILEK AHVKKPVKRL WISSVTDKAI KDGFKQLKDG
160 170 180 190 200
KRYETLYAAA AARAEADWYV GINGTRALTT KHNAQLSCGR VQTPTLAILA
210 220 230 240 250
DREEAIRKFV PKPYHTISVE TKDGVTFRWA DRRSKEERIF DDKRAKTLQE
260 270 280 290 300
KLQQSAIVID SVNAKTKRTP PPPLYDLTEL QREANKRFGY SAKETLAALQ
310 320 330 340 350
RLYEQHKAVT YPRTDSRYLS SDLVETLKER LHAVDVQPFR KMVANAKKQG
360 370 380 390 400
LANAKRQMVN DAKVSDHHAL IPTEQPQSAS AMSDKEAKLY HLIVRRFLAN
410 420 430 440 450
FFPPSESELT SVEATANGEL LRAKGERLVS AGWRGQETGE EDEALARLLP
460 470 480 490 500
AMSKGDKKAV KWVGMTQGKT SPPPRFNEGT LLAAMEKPAQ HMEAKDETIK
510 520 530 540 550
KTLAQAGGIG TVATRADIIE KLFSSFYIEK KGKDLYITSK GKQLLELVPA
560 570 580 590 600
ELKSPALTAE WEQRLEKIVQ GKEQKQMFIA EMKEYAKAVV AQVKADTATF
610 620 630 640 650
RHDNKTGEKC PECGKFLLEV NGKKGKMRVC QDRECGYRKN IAMTTNARCP
660 670 680 690 700
KCKKKLELRG EGEGQVFACV CGHREKKAQF EQRRKQNKHK NVSKREVQSY
710 720
MKKQNKQDQF ANSALADQLA KLGLKGDDS
Length:729
Mass (Da):82,115
Last modified:November 23, 2004 - v1
Checksum:i538DCA474A448178
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA Translation: BAD66484.1
RefSeqiWP_011248787.1, NC_006582.1

Genome annotation databases

EnsemblBacteriaiBAD66484; BAD66484; ABC3953
GeneIDi34046101
KEGGibcl:ABC3953

Similar proteinsi

Entry informationi

Entry nameiTOP3_BACSK
AccessioniPrimary (citable) accession number: Q5WAX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 23, 2004
Last modified: February 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome