ID   Q5WAE3_SHOC1            Unreviewed;       836 AA.
AC   Q5WAE3;
DT   23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT   23-NOV-2004, sequence version 1.
DT   27-MAR-2024, entry version 96.
DE   SubName: Full=Serine phosphatase stage II sporulation protein E {ECO:0000313|EMBL:BAD62644.1};
DE            EC=3.1.3.16 {ECO:0000313|EMBL:BAD62644.1};
GN   Name=spoIIE {ECO:0000313|EMBL:BAD62644.1};
GN   OrderedLocusNames=ABC0101 {ECO:0000313|EMBL:BAD62644.1};
OS   Shouchella clausii (strain KSM-K16) (Alkalihalobacillus clausii).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
OX   NCBI_TaxID=66692 {ECO:0000313|EMBL:BAD62644.1, ECO:0000313|Proteomes:UP000001168};
RN   [1] {ECO:0000313|EMBL:BAD62644.1, ECO:0000313|Proteomes:UP000001168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16 {ECO:0000313|EMBL:BAD62644.1,
RC   ECO:0000313|Proteomes:UP000001168};
RA   Hakamada Y., Kobayashi T., Hitomi J., Kawai S., Ito S.;
RT   "Molecular cloning and nucleotide sequence of the gene for an alkaline
RT   protease from the alkalophilic Bacillus sp. KSM-K16.";
RL   J. Ferment. Bioeng. 78:105-108(1994).
RN   [2] {ECO:0000313|EMBL:BAD62644.1, ECO:0000313|Proteomes:UP000001168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16 {ECO:0000313|EMBL:BAD62644.1,
RC   ECO:0000313|Proteomes:UP000001168};
RX   PubMed=7632397; DOI=10.1007/s002530050437;
RA   Kobayashi T., Hakamada Y., Adachi S., Hitomi J., Yoshimatsu T., Koike K.,
RA   Kawai S., Ito S.;
RT   "Purification and properties of an alkaline protease from alkalophilic
RT   Bacillus sp. KSM-K16.";
RL   Appl. Microbiol. Biotechnol. 43:473-481(1995).
RN   [3] {ECO:0000313|EMBL:BAD62644.1, ECO:0000313|Proteomes:UP000001168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16 {ECO:0000313|EMBL:BAD62644.1,
RC   ECO:0000313|Proteomes:UP000001168};
RX   PubMed=9278275; DOI=10.1093/protein/10.6.627;
RA   Shirai T., Suzuki A., Yamane T., Ashida T., Kobayashi T., Ito S.;
RT   "High-resolution crystal structure of M-protease: phylogeny aided analysis
RT   of the high-alkaline adaptation mechanism.";
RL   Protein Eng. 10:627-634(1997).
RN   [4] {ECO:0000313|Proteomes:UP000001168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16 {ECO:0000313|Proteomes:UP000001168};
RA   Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y.,
RA   Kawai S., Ito S., Horikoshi K.;
RT   "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-
RT   K16.";
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:BAD62644.1, ECO:0000313|Proteomes:UP000001168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16 {ECO:0000313|EMBL:BAD62644.1,
RC   ECO:0000313|Proteomes:UP000001168};
RX   PubMed=17429572; DOI=10.1007/s00792-007-0074-1;
RA   Kageyama Y., Takaki Y., Shimamura S., Nishi S., Nogi Y., Uchimura K.,
RA   Kobayashi T., Hitomi J., Ozaki K., Kawai S., Ito S., Horikoshi K.;
RT   "Intragenomic diversity of the V1 regions of 16S rRNA genes in high-
RT   alkaline protease-producing Bacillus clausii spp.";
RL   Extremophiles 11:597-603(2007).
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DR   EMBL; AP006627; BAD62644.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5WAE3; -.
DR   STRING; 66692.ABC0101; -.
DR   KEGG; bcl:ABC0101; -.
DR   eggNOG; COG2208; Bacteria.
DR   HOGENOM; CLU_017349_0_0_9; -.
DR   Proteomes; UP000001168; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   InterPro; IPR014221; SpoII_E.
DR   InterPro; IPR045768; SpoIIE_N.
DR   NCBIfam; TIGR02865; spore_II_E; 1.
DR   PANTHER; PTHR43156:SF12; STAGE II SPORULATION PROTEIN E; 1.
DR   PANTHER; PTHR43156; STAGE II SPORULATION PROTEIN E-RELATED; 1.
DR   Pfam; PF07228; SpoIIE; 1.
DR   Pfam; PF19732; SpoIIE_N; 1.
DR   SMART; SM00331; PP2C_SIG; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS51746; PPM_2; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000313|EMBL:BAD62644.1};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001168};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        39..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        128..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        153..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        194..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        225..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        257..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          593..804
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000259|PROSITE:PS51746"
FT   REGION          812..836
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   836 AA;  91911 MW;  3D05648C5EE6F382 CRC64;
     MIKTSEEMGT AKVAEKLNGI WTKCASSGQA LKSGAKTVLY DWGIFIAILG FLLGRAMILS
     ELTPFILPFL AAVFLLRRSQ SLIAAASLLA GAVFSFHGQF IFAIAGIGFF LILYKCMKMF
     MKHPAKSLPY LVFSASIATR LSLVFLTEGG LSQYAMMMAT VEAALSFILT MIFIQSIPLV
     TGKRGGQALR NEEIICLIIL LASVMTGTVG WTINEAVLQH SFASYLVLVF AFVGGAAIGS
     TVGVVTGLIL SLASLASLYQ MSLLAFAGLL GGLLKEGKRI GVSLGLLVGT LLIGMYGQGG
     GLGASVSESA IAITLFLLTP KSWLTKVARY IPGTVEHSQE QQQYLRKVRD ATAGKVERFS
     SLFQTLSNSF HTPSKNEEEE HDHEVDVLLS RVTEKTCQTC MLKEKCWVQN FNATYDSMKQ
     MVQESEVHGT VVDPKLQRQW RSHCRKPDQV MAALNAEVNH YRANKELKRQ VLESQRLVAD
     QLLGVSRVMG DFAKEIQKEK QPHVIQEEDM VDALRNAGLE VGHIDIYSME SGSIEIEMSV
     LCSHENGEAE KIIAPMLSDL VKETIVLMRE EPGFYSNGYS HISFGSAKPF AVETGIAKVA
     KGGEWLSGDN YAMIKLNSEK FAVAISDGMG NGEKAHLESS ETLKLLQKVL QSGIEETVAI
     KSVNSILSLR NTEEMFSTLD LAMIDMQDAG AKFLKIGSTP SFIKRKDHVI KIEAGNLPMG
     IFHEFEVDVV SEQLKPGDLL IMCSDGVFDA KRQVENKEQW MKRMIKEIET DDPQEVADVL
     LEKVIRSEKG IVIEDDMTIV VTQLKHNTPK WSSIPIHPKT TKNKKSAPFY KQATGT
//