Q5WA72 (PDI15_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase-like 1-5
Short name=OsPDIL1-5
EC=5.3.4.1

Alternative name(s):
Protein disulfide isomerase-like 3-1
Short name=OsPDIL3-1

Gene names

Name:	PDIL1-5
Synonyms:	PDIL3-1
Ordered Locus Names:	Os06g0163400, LOC_Os06g06790
ORF Names:	P0681F10.44

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds. May play a role in storage protein biogenesis By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Probable.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 533	511	Protein disulfide isomerase-like 1-5		PRO_0000400032
Regions
	Domain	51 – 196	146	Thioredoxin 1
	Domain	387 – 516	130	Thioredoxin 2
	Motif	530 – 533	4	Prevents secretion from ER By similarity
Sites
	Active site	97	1	Nucleophile By similarity
	Active site	436	1	Nucleophile By similarity
	Active site	439	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Disulfide bond	436 ↔ 439		Redox-active By similarity
Experimental info
	Sequence conflict	208	1	A → T in AK073970. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5WA72 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: F435D08CE96793B7	FASTA	533	59,032
	10 20 30 40 50 60 MRARRVVAAA AVLLLFAVVA VARLDLDDDG DDSEVLDELL AVDEEEERGE LGGGGEAAAA 70 80 90 100 110 120 EAVRRAQSMV LVLDNDNARR AVEENAEVLL LGYAPWCERS AQLMPRFAEA AAALRAMGSA 130 140 150 160 170 180 VAFAKLDGER YPKAASAVGV KGFPTVLLFV NGTEHQFTGL HTKDAIVTWV RKKTGAPASR 190 200 210 220 230 240 IQSKDSAEEF LKKDQTFAVG LFKNFEGAEY EEFVKAATSE NEVQFVETND RNVAKILFPG 250 260 270 280 290 300 IASEEQFLGL VKSEPEKFEK FNGAFEEKEI IQFVELNKFP LITVFTDLNS GKVYGSPIKL 310 320 330 340 350 360 QVFTFAEAYD FEDLESMIQE VARGFKTKIM LIYVDTAEEK LAKPFLTLYG LEPEKPTVTA 370 380 390 400 410 420 FDTSKGTKYL MEAEINAKNL QDFCLSLLEG TLPPYFRSEP VPEEKGPIEK VVGRTFDSSV 430 440 450 460 470 480 LESPQNVFLE VHAPWCVDCE AISKNVEKLA KHFNDLGQTN LKFARIDASV NEHPKLQINN 490 500 510 520 530 YPTLLLYPAQ DKSNPIKLSK KSNLKDMAKF VKEKLQIADV ETVAAGDIVK DEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins." Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S. Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)." d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M. BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026295 Genomic DNA. Translation: BAD67648.1. AP008212 Genomic DNA. Translation: BAF18810.1. AK073970 mRNA. No translation available.
RefSeq	NP_001056896.1. NM_001063431.1.
UniGene	Os.4371.
3D structure databases
ProteinModelPortal	Q5WA72.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g06790.1; LOC_Os06g06790.1; LOC_Os06g06790.
GeneID	4340223.
KEGG	dosa:Os06t0163400-01. osa:4340223.
Organism-specific databases
Gramene	Q5WA72.
Phylogenomic databases
eggNOG	COG0526.
KO	K09580.
OMA	NEIQFVE.
ProtClustDB	CLSN2680753.
Gene expression databases
ArrayExpress	Q5WA72.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDI15_ORYSJ

Accession

Primary (citable) accession number: Q5WA72

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program