ID   MAN5_ORYSJ              Reviewed;         491 AA.
AC   Q5W6G0; A0A0N7KKI9;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   27-MAR-2024, entry version 96.
DE   RecName: Full=Putative mannan endo-1,4-beta-mannosidase 5;
DE            EC=3.2.1.78;
DE   AltName: Full=Beta-mannanase 5;
DE   AltName: Full=Endo-beta-1,4-mannanase 5;
DE   AltName: Full=OsMAN5;
DE   Flags: Precursor;
GN   Name=MAN5; OrderedLocusNames=Os05g0319100, LOC_Os05g25480;
GN   ORFNames=OSJNBb0006B22.2, OSJNBb0059K16.9;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA   Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT   "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL   Funct. Integr. Genomics 7:1-16(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expression not detected.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAV44080.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAV44120.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AC134344; AAV44120.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AC136224; AAV44080.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014961; BAS93345.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5W6G0; -.
DR   SMR; Q5W6G0; -.
DR   STRING; 39947.Q5W6G0; -.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   GlyCosmos; Q5W6G0; 2 sites, No reported glycans.
DR   PaxDb; 39947-Q5W6G0; -.
DR   EnsemblPlants; Os05t0319100-00; Os05t0319100-00; Os05g0319100.
DR   Gramene; Os05t0319100-00; Os05t0319100-00; Os05g0319100.
DR   eggNOG; ENOG502QU0Z; Eukaryota.
DR   HOGENOM; CLU_031603_0_0_1; -.
DR   InParanoid; Q5W6G0; -.
DR   OMA; TPQPFRM; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451:SF54; MANNAN ENDO-1,4-BETA-MANNOSIDASE 6; 1.
DR   PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   Genevisible; Q5W6G0; OS.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..491
FT                   /note="Putative mannan endo-1,4-beta-mannosidase 5"
FT                   /id="PRO_0000277486"
FT   ACT_SITE        249
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   ACT_SITE        372
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   BINDING         134
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         248
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         330
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         416
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   CARBOHYD        385
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        471
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   491 AA;  53465 MW;  4494130235C34371 CRC64;
     METSYREEEA RRKASLLHCI FFFLLGALAM AAAIAVLHES SYWEWRCNRL TDIVVDGDDG
     DGPSSSEVVD GGGEWGMVRT RGAQFVVGGG RPFYVNGFNT YWLMVLAVDP STRGKVTEVF
     RQAAAVGLTV CRTWAFNDGG WRALQKSPGV YDEEVFKALD FVVSEARKHK IRLILPLINN
     WDDYGGKAQY VRWAQAAAAG AGADAFFSDE TVRGYFKSHV TAVLTRVNAY TGVAYRDDPT
     IMAWELMNEP RCASDPTGDT LQAWIAEMAF HVKSVDPAHL LGVGAEGFYG PSSPPARLRV
     NPNADVALAG ADFVRNHRVL GVDFASVHVY PDTWLPAGAT KEAQLRFATS WVEAHIADAE
     GALGGMPVLF AEFGVSTRGA RAAFNATSRD AFIEAVYGAM LRSTRRGGGG AGALLWQVFP
     EGTDYMDDGY AVVLPRAAAT AGIVAAHSRR LQSFNSRCAW SCRWGCNKRD NDTAETTTAE
     ADVDVSFHHE L
//