Q5W6G0 (MAN5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative mannan endo-1,4-beta-mannosidase 5
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 5
Endo-beta-1,4-mannanase 5
OsMAN5

Gene names

Name:	MAN5
Ordered Locus Names:	Os05g0319100, LOC_Os05g25480
ORF Names:	OSJNBb0006B22.2, OSJNBb0059K16.9

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expression not detected.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence AAV44080.1 differs from that shown. Reason: Erroneous initiation. The sequence AAV44120.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 491	460	Putative mannan endo-1,4-beta-mannosidase 5		PRO_0000277486
Sites
	Active site	249	1	Proton donor By similarity
	Active site	372	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	385	1	N-linked (GlcNAc...) Potential
	Glycosylation	471	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5W6G0 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 4494130235C34371	FASTA	491	53,465
	10 20 30 40 50 60 METSYREEEA RRKASLLHCI FFFLLGALAM AAAIAVLHES SYWEWRCNRL TDIVVDGDDG 70 80 90 100 110 120 DGPSSSEVVD GGGEWGMVRT RGAQFVVGGG RPFYVNGFNT YWLMVLAVDP STRGKVTEVF 130 140 150 160 170 180 RQAAAVGLTV CRTWAFNDGG WRALQKSPGV YDEEVFKALD FVVSEARKHK IRLILPLINN 190 200 210 220 230 240 WDDYGGKAQY VRWAQAAAAG AGADAFFSDE TVRGYFKSHV TAVLTRVNAY TGVAYRDDPT 250 260 270 280 290 300 IMAWELMNEP RCASDPTGDT LQAWIAEMAF HVKSVDPAHL LGVGAEGFYG PSSPPARLRV 310 320 330 340 350 360 NPNADVALAG ADFVRNHRVL GVDFASVHVY PDTWLPAGAT KEAQLRFATS WVEAHIADAE 370 380 390 400 410 420 GALGGMPVLF AEFGVSTRGA RAAFNATSRD AFIEAVYGAM LRSTRRGGGG AGALLWQVFP 430 440 450 460 470 480 EGTDYMDDGY AVVLPRAAAT AGIVAAHSRR LQSFNSRCAW SCRWGCNKRD NDTAETTTAE 490 ADVDVSFHHE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC134344 Genomic DNA. Translation: AAV44120.1. Different initiation. AC136224 Genomic DNA. Translation: AAV44080.1. Different initiation.
3D structure databases
ProteinModelPortal	Q5W6G0.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os05t0319100-00.
Organism-specific databases
Gramene	Q5W6G0.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169952.
Gene expression databases
ArrayExpress	Q5W6G0.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN5_ORYSJ

Accession

Primary (citable) accession number: Q5W6G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program