Reviewed, UniProtKB/Swiss-Prot Q5W676 (HXK5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Hexokinase-5
    EC=2.7.1.1
Alternative name(s):
    Hexokinase I

Gene names

Name:	HXK5
Ordered Locus Names:	Os05g0522500, LOC_Os05g44760
ORF Names:	OJ1087_C03.12, OSJNBa0075A10.5

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol By similarity. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast outer membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat. Ref.1
Developmental stage	Expressed during flower development until 15 days after flowering. Ref.1
Induction	By glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Hexokinase-5		PRO_0000247568
Regions
	Transmembrane	4 – 24	21	Potential
	Nucleotide binding	110 – 115	6	ATP Potential
	Region	180 – 206	27	Glucose-binding Potential
	Compositional bias	25 – 30	6	Poly-Arg

Sequences

	Sequence		Length	Mass (Da)
	Q5W676-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 9A3A685AF1E28D7A	FASTA	507	54,659
	10 20 30 40 50 60 MGKAAAVGTA VVVAAAVGVA VVLARRRRRR DLELVEGAAA ERKRKVAAVI EDVEHALSTP 70 80 90 100 110 120 TALLRGISDA MVTEMERGLR GDSHAMVKML ITYVDNLPTG NEQGLFYALD LGGTNFRVLR 130 140 150 160 170 180 VQLGGKEKRV VQQQYEEVSI PPHLMVGTSM ELFDFIASAL SKFVDTEGDD FHLPEGRQRE 190 200 210 220 230 240 LGFTFSFPVS QTSISSGTLI KWTKGFSIND AVGEDVVSEL GKAMERQGLD MKIAALVNDT 250 260 270 280 290 300 VGTLAGGRYA DNSVVAAIIL GTGTNAAYVE NANAIPKWTG LLPRSGNMVI NTEWGSFKSD 310 320 330 340 350 360 KLPLSEFDKA MDFESLNPGE QIYEKLISGM YLGEIVRRIL LKLAHDAALF GDVVPSKLEQ 370 380 390 400 410 420 PFVLRTPDMS AMHHDSSHDL KTVGAKLKDI VGVPDTSLEV RYITSHICDI VAERAARLAA 430 440 450 460 470 480 AGIYGVLKKL GRDKMPKDGS KMPRTVIALD GGLYEHYKKF SSCLESTLTD LLGDDVSSSV 490 500 VTKLANDGSG IGAALLAASH SQYAEID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"Rice hexokinase I mRNA." Wu P., Jiang H.-W., Yi K.-K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116387 mRNA. Translation: AAZ93622.1. AF372831 mRNA. Translation: AAK51559.1. AC118284 Genomic DNA. Translation: AAV59322.1. AC144740 Genomic DNA. Translation: AAV44032.1.
RefSeq	NP_001056082.1.
UniGene	Os.3293
3D structure databases
SMR	Q5W676. Positions 50-502.
ModBase	Search...
Genome annotation databases
GeneID	4339361.
KEGG	osa:4339361.
Organism-specific databases
Gramene	Q5W676.
Phylogenomic databases
PhylomeDB	Q5W676.
Enzyme and pathway databases
BRENDA	2.7.1.1. 1975.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK5_ORYSJ

Accession

Primary (citable) accession number: Q5W676
Secondary accession number(s): Q94JW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 7, 2004
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)