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Q5W416

- PSB8_CANFA

UniProt

Q5W416 - PSB8_CANFA

Protein

Proteasome subunit beta type-8

Gene

PSMB8

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity. Required for adipocyte differentiation By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei72 – 732Cleavage; by autocatalysisBy similarity
    Active sitei73 – 731NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. antigen processing and presentation Source: Ensembl
    2. fat cell differentiation Source: UniProtKB
    3. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Keywords - Biological processi

    Differentiation, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_175310. Separation of Sister Chromatids.
    REACT_182252. ER-Phagosome pathway.
    REACT_182255. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_182274. Activation of NF-kappaB in B cells.
    REACT_182278. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_182626. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_205131. Asymmetric localization of PCP proteins.
    REACT_210008. degradation of DVL.
    REACT_227902. degradation of AXIN.

    Protein family/group databases

    MEROPSiT01.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-8 (EC:3.4.25.1)
    Alternative name(s):
    Proteasome subunit beta-5i
    Gene namesi
    Name:PSMB8
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Chromosome 12

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB
    4. spermatoproteasome complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 7272Removed in mature formBy similarityPRO_0000026595Add
    BLAST
    Chaini73 – 276204Proteasome subunit beta type-8PRO_0000026596Add
    BLAST

    Post-translational modificationi

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

    Keywords - PTMi

    Zymogen

    Expressioni

    Inductioni

    Up-regulated by interferon gamma (at protein level).

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000001185.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5W416.
    SMRiQ5W416. Positions 73-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00510000046395.
    HOGENOMiHOG000091082.
    HOVERGENiHBG108297.
    InParanoidiQ5W416.
    KOiK02740.
    OMAiSDLMHQY.
    OrthoDBiEOG7FNC86.
    TreeFamiTF106223.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5W416-1 [UniParc]FASTAAdd to Basket

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    MALLEVCGAP RGLRKACAVP ALGSQLRSDP GHYSFSLRAP ELAVPRGMQP    50
    TEFFQSLGEN GEKNIRKEMV HGTTTLAFKF QHGVIVAVDS RATAGNYISS 100
    SRVNKVIEIN PSLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA 150
    ASKLLSNMVY QYRGMDLSMG SMICGWDKKG PGLYYVDQNG TRLSGNMFST 200
    GSGSTYAYGV MDSGYQPSLS PEEAYELGRR AITYATHRDS YSGGIINMYH 250
    MKEDGWVKVE STDVNELLHQ YQEANQ 276
    Length:276
    Mass (Da):30,499
    Last modified:December 7, 2004 - v1
    Checksum:iD7CA845F17D3AF4F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ630364 Genomic DNA. Translation: CAH63452.1.
    RefSeqiNP_001041550.1. NM_001048085.1.
    XP_005627104.1. XM_005627047.1.
    UniGeneiCfa.12298.

    Genome annotation databases

    EnsembliENSCAFT00000001293; ENSCAFP00000001185; ENSCAFG00000000829.
    GeneIDi474865.
    KEGGicfa:474865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ630364 Genomic DNA. Translation: CAH63452.1 .
    RefSeqi NP_001041550.1. NM_001048085.1.
    XP_005627104.1. XM_005627047.1.
    UniGenei Cfa.12298.

    3D structure databases

    ProteinModelPortali Q5W416.
    SMRi Q5W416. Positions 73-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000001185.

    Protein family/group databases

    MEROPSi T01.015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCAFT00000001293 ; ENSCAFP00000001185 ; ENSCAFG00000000829 .
    GeneIDi 474865.
    KEGGi cfa:474865.

    Organism-specific databases

    CTDi 5696.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00510000046395.
    HOGENOMi HOG000091082.
    HOVERGENi HBG108297.
    InParanoidi Q5W416.
    KOi K02740.
    OMAi SDLMHQY.
    OrthoDBi EOG7FNC86.
    TreeFami TF106223.

    Enzyme and pathway databases

    Reactomei REACT_175310. Separation of Sister Chromatids.
    REACT_182252. ER-Phagosome pathway.
    REACT_182255. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_182274. Activation of NF-kappaB in B cells.
    REACT_182278. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_182626. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_205131. Asymmetric localization of PCP proteins.
    REACT_210008. degradation of DVL.
    REACT_227902. degradation of AXIN.

    Miscellaneous databases

    NextBioi 20850809.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species."
      Debenham S.L., Hart E.A., Ashurst J.L., Howe K.L., Quail M.A., Ollier W.E.R., Binns M.M.
      Genomics 85:48-59(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Doberman pinscher.

    Entry informationi

    Entry nameiPSB8_CANFA
    AccessioniPrimary (citable) accession number: Q5W416
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3