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Protein

Proteasome subunit beta type-8

Gene

PSMB8

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides (By similarity). Required for adipocyte differentiation (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Differentiation, Immunity

Enzyme and pathway databases

ReactomeiR-CFA-1169091. Activation of NF-kappaB in B cells.
R-CFA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-CFA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-CFA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-CFA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-CFA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-CFA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-CFA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-CFA-195253. Degradation of beta-catenin by the destruction complex.
R-CFA-202424. Downstream TCR signaling.
R-CFA-2467813. Separation of Sister Chromatids.
R-CFA-2871837. FCERI mediated NF-kB activation.
R-CFA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-CFA-350562. Regulation of ornithine decarboxylase (ODC).
R-CFA-382556. ABC-family proteins mediated transport.
R-CFA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-CFA-4608870. Asymmetric localization of PCP proteins.
R-CFA-4641257. Degradation of AXIN.
R-CFA-4641258. Degradation of DVL.
R-CFA-5358346. Hedgehog ligand biogenesis.
R-CFA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-CFA-5607764. CLEC7A (Dectin-1) signaling.
R-CFA-5610780. Degradation of GLI1 by the proteasome.
R-CFA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-CFA-5632684. Hedgehog 'on' state.
R-CFA-5658442. Regulation of RAS by GAPs.
R-CFA-5668541. TNFR2 non-canonical NF-kB pathway.
R-CFA-5676590. NIK-->noncanonical NF-kB signaling.
R-CFA-5687128. MAPK6/MAPK4 signaling.
R-CFA-5689603. UCH proteinases.
R-CFA-5689880. Ub-specific processing proteases.
R-CFA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-CFA-68949. Orc1 removal from chromatin.
R-CFA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-CFA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-CFA-69481. G2/M Checkpoints.
R-CFA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CFA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-CFA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-CFA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta-5i
Gene namesi
Name:PSMB8
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 12

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000265951 – 72Removed in mature formBy similarityAdd BLAST72
ChainiPRO_000002659673 – 276Proteasome subunit beta type-8Add BLAST204

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72 – 73Cleavage; by autolysisBy similarity2

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ5W416.

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level).

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001185.

Structurei

3D structure databases

ProteinModelPortaliQ5W416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiQ5W416.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG091G0BPS.
TreeFamiTF106223.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5W416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLEVCGAP RGLRKACAVP ALGSQLRSDP GHYSFSLRAP ELAVPRGMQP
60 70 80 90 100
TEFFQSLGEN GEKNIRKEMV HGTTTLAFKF QHGVIVAVDS RATAGNYISS
110 120 130 140 150
SRVNKVIEIN PSLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMVY QYRGMDLSMG SMICGWDKKG PGLYYVDQNG TRLSGNMFST
210 220 230 240 250
GSGSTYAYGV MDSGYQPSLS PEEAYELGRR AITYATHRDS YSGGIINMYH
260 270
MKEDGWVKVE STDVNELLHQ YQEANQ
Length:276
Mass (Da):30,499
Last modified:December 7, 2004 - v1
Checksum:iD7CA845F17D3AF4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ630364 Genomic DNA. Translation: CAH63452.1.
RefSeqiNP_001041550.1. NM_001048085.1.
XP_005627104.1. XM_005627047.2.
UniGeneiCfa.12298.

Genome annotation databases

EnsembliENSCAFT00000001293; ENSCAFP00000001185; ENSCAFG00000000829.
GeneIDi474865.
KEGGicfa:474865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ630364 Genomic DNA. Translation: CAH63452.1.
RefSeqiNP_001041550.1. NM_001048085.1.
XP_005627104.1. XM_005627047.2.
UniGeneiCfa.12298.

3D structure databases

ProteinModelPortaliQ5W416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001185.

Protein family/group databases

MEROPSiT01.015.

Proteomic databases

PaxDbiQ5W416.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000001293; ENSCAFP00000001185; ENSCAFG00000000829.
GeneIDi474865.
KEGGicfa:474865.

Organism-specific databases

CTDi5696.

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiQ5W416.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG091G0BPS.
TreeFamiTF106223.

Enzyme and pathway databases

ReactomeiR-CFA-1169091. Activation of NF-kappaB in B cells.
R-CFA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-CFA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-CFA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-CFA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-CFA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-CFA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-CFA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-CFA-195253. Degradation of beta-catenin by the destruction complex.
R-CFA-202424. Downstream TCR signaling.
R-CFA-2467813. Separation of Sister Chromatids.
R-CFA-2871837. FCERI mediated NF-kB activation.
R-CFA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-CFA-350562. Regulation of ornithine decarboxylase (ODC).
R-CFA-382556. ABC-family proteins mediated transport.
R-CFA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-CFA-4608870. Asymmetric localization of PCP proteins.
R-CFA-4641257. Degradation of AXIN.
R-CFA-4641258. Degradation of DVL.
R-CFA-5358346. Hedgehog ligand biogenesis.
R-CFA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-CFA-5607764. CLEC7A (Dectin-1) signaling.
R-CFA-5610780. Degradation of GLI1 by the proteasome.
R-CFA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-CFA-5632684. Hedgehog 'on' state.
R-CFA-5658442. Regulation of RAS by GAPs.
R-CFA-5668541. TNFR2 non-canonical NF-kB pathway.
R-CFA-5676590. NIK-->noncanonical NF-kB signaling.
R-CFA-5687128. MAPK6/MAPK4 signaling.
R-CFA-5689603. UCH proteinases.
R-CFA-5689880. Ub-specific processing proteases.
R-CFA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-CFA-68949. Orc1 removal from chromatin.
R-CFA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-CFA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-CFA-69481. G2/M Checkpoints.
R-CFA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CFA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-CFA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-CFA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB8_CANLF
AccessioniPrimary (citable) accession number: Q5W416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.