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Q5W416

- PSB8_CANFA

UniProt

Q5W416 - PSB8_CANFA

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Protein

Proteasome subunit beta type-8

Gene

PSMB8

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity. Required for adipocyte differentiation By similarity.By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei72 – 732Cleavage; by autocatalysisBy similarity
Active sitei73 – 731NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. antigen processing and presentation Source: Ensembl
  2. fat cell differentiation Source: UniProtKB
  3. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Differentiation, Immunity

Enzyme and pathway databases

ReactomeiREACT_175310. Separation of Sister Chromatids.
REACT_182252. ER-Phagosome pathway.
REACT_182255. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_182274. Activation of NF-kappaB in B cells.
REACT_182278. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_182626. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_205131. Asymmetric localization of PCP proteins.
REACT_210008. degradation of DVL.
REACT_227902. degradation of AXIN.

Protein family/group databases

MEROPSiT01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta-5i
Gene namesi
Name:PSMB8
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Chromosome 12

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: UniProtKB-KW
  4. proteasome core complex Source: UniProtKB
  5. spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 7272Removed in mature formBy similarityPRO_0000026595Add
BLAST
Chaini73 – 276204Proteasome subunit beta type-8PRO_0000026596Add
BLAST

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

Keywords - PTMi

Zymogen

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level).

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001185.

Structurei

3D structure databases

ProteinModelPortaliQ5W416.
SMRiQ5W416. Positions 73-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiQ5W416.
KOiK02740.
OMAiSDLMHQY.
OrthoDBiEOG7FNC86.
TreeFamiTF106223.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5W416-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLEVCGAP RGLRKACAVP ALGSQLRSDP GHYSFSLRAP ELAVPRGMQP
60 70 80 90 100
TEFFQSLGEN GEKNIRKEMV HGTTTLAFKF QHGVIVAVDS RATAGNYISS
110 120 130 140 150
SRVNKVIEIN PSLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMVY QYRGMDLSMG SMICGWDKKG PGLYYVDQNG TRLSGNMFST
210 220 230 240 250
GSGSTYAYGV MDSGYQPSLS PEEAYELGRR AITYATHRDS YSGGIINMYH
260 270
MKEDGWVKVE STDVNELLHQ YQEANQ
Length:276
Mass (Da):30,499
Last modified:December 7, 2004 - v1
Checksum:iD7CA845F17D3AF4F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ630364 Genomic DNA. Translation: CAH63452.1.
RefSeqiNP_001041550.1. NM_001048085.1.
XP_005627104.1. XM_005627047.1.
UniGeneiCfa.12298.

Genome annotation databases

EnsembliENSCAFT00000001293; ENSCAFP00000001185; ENSCAFG00000000829.
GeneIDi474865.
KEGGicfa:474865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ630364 Genomic DNA. Translation: CAH63452.1 .
RefSeqi NP_001041550.1. NM_001048085.1.
XP_005627104.1. XM_005627047.1.
UniGenei Cfa.12298.

3D structure databases

ProteinModelPortali Q5W416.
SMRi Q5W416. Positions 73-271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000001185.

Protein family/group databases

MEROPSi T01.015.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCAFT00000001293 ; ENSCAFP00000001185 ; ENSCAFG00000000829 .
GeneIDi 474865.
KEGGi cfa:474865.

Organism-specific databases

CTDi 5696.

Phylogenomic databases

eggNOGi COG0638.
GeneTreei ENSGT00510000046395.
HOGENOMi HOG000091082.
HOVERGENi HBG108297.
InParanoidi Q5W416.
KOi K02740.
OMAi SDLMHQY.
OrthoDBi EOG7FNC86.
TreeFami TF106223.

Enzyme and pathway databases

Reactomei REACT_175310. Separation of Sister Chromatids.
REACT_182252. ER-Phagosome pathway.
REACT_182255. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_182274. Activation of NF-kappaB in B cells.
REACT_182278. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_182626. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_205131. Asymmetric localization of PCP proteins.
REACT_210008. degradation of DVL.
REACT_227902. degradation of AXIN.

Miscellaneous databases

NextBioi 20850809.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species."
    Debenham S.L., Hart E.A., Ashurst J.L., Howe K.L., Quail M.A., Ollier W.E.R., Binns M.M.
    Genomics 85:48-59(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Doberman pinscher.

Entry informationi

Entry nameiPSB8_CANFA
AccessioniPrimary (citable) accession number: Q5W416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3