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Q5W416 (PSB8_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-8

EC=3.4.25.1
Alternative name(s):
Proteasome subunit beta-5i
Gene names
Name:PSMB8
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity. Required for adipocyte differentiation By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Induction

Up-regulated by interferon gamma (at protein level).

Post-translational modification

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 7272Removed in mature form By similarity
PRO_0000026595
Chain73 – 276204Proteasome subunit beta type-8
PRO_0000026596

Sites

Active site731Nucleophile By similarity
Site72 – 732Cleavage; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5W416 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: D7CA845F17D3AF4F

FASTA27630,499
        10         20         30         40         50         60 
MALLEVCGAP RGLRKACAVP ALGSQLRSDP GHYSFSLRAP ELAVPRGMQP TEFFQSLGEN 

        70         80         90        100        110        120 
GEKNIRKEMV HGTTTLAFKF QHGVIVAVDS RATAGNYISS SRVNKVIEIN PSLLGTMSGC 

       130        140        150        160        170        180 
AADCQYWERL LAKECRLYYL RNGERISVSA ASKLLSNMVY QYRGMDLSMG SMICGWDKKG 

       190        200        210        220        230        240 
PGLYYVDQNG TRLSGNMFST GSGSTYAYGV MDSGYQPSLS PEEAYELGRR AITYATHRDS 

       250        260        270 
YSGGIINMYH MKEDGWVKVE STDVNELLHQ YQEANQ 

« Hide

References

[1]"Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species."
Debenham S.L., Hart E.A., Ashurst J.L., Howe K.L., Quail M.A., Ollier W.E.R., Binns M.M.
Genomics 85:48-59(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Doberman pinscher.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ630364 Genomic DNA. Translation: CAH63452.1.
RefSeqNP_001041550.1. NM_001048085.1.
XP_005627104.1. XM_005627047.1.
UniGeneCfa.12298.

3D structure databases

ProteinModelPortalQ5W416.
SMRQ5W416. Positions 73-271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9615.ENSCAFP00000001185.

Protein family/group databases

MEROPST01.015.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCAFT00000001293; ENSCAFP00000001185; ENSCAFG00000000829.
GeneID474865.
KEGGcfa:474865.

Organism-specific databases

CTD5696.

Phylogenomic databases

eggNOGCOG0638.
GeneTreeENSGT00510000046395.
HOGENOMHOG000091082.
HOVERGENHBG108297.
InParanoidQ5W416.
KOK02740.
OMASDLMHQY.
OrthoDBEOG7FNC86.
TreeFamTF106223.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20850809.

Entry information

Entry namePSB8_CANFA
AccessionPrimary (citable) accession number: Q5W416
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries