Q5W0U4 (BSPRY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
B box and SPRY domain-containing protein

Gene names

Name:

BSPRY

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate epithelial calcium transport by inhibiting TRPV5 activity By similarity.
Subunit structure	Interacts with TRPV5 and TRPV6. Interacts with YWHAZ/14-3-3 protein zeta By similarity.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Contains 1 B box-type zinc finger. Contains 1 B30.2/SPRY domain.
Sequence caution	The sequence BAA90980.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Calcium Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell leading edge Inferred from electronic annotation. Source: Compara cytoplasm Inferred from direct assay. Source: HPA focal adhesion Inferred from direct assay. Source: HPA membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5W0U4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5W0U4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     187-193: TEEAEGI → HRGHTDR
     194-402: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	B box and SPRY domain-containing protein		PRO_0000244257
Regions
	Domain	212 – 402	191	B30.2/SPRY
	Zinc finger	17 – 65	49	B box-type
Natural variations
	Alternative sequence	187 – 193	7	TEEAEGI → HRGHTDR in isoform 2.		VSP_019527
	Alternative sequence	194 – 402	209	Missing in isoform 2.		VSP_019528
	Natural variant	261	1	A → P. Corresponds to variant rs34089316 [ dbSNP | Ensembl ].		VAR_048397
	Natural variant	293	1	Q → H. Corresponds to variant rs818711 [ dbSNP | Ensembl ].		VAR_026882
	Natural variant	374	1	T → I. Ref.1 Ref.5 Corresponds to variant rs3088235 [ dbSNP | Ensembl ].		VAR_026883
Experimental info
	Sequence conflict	100	1	V → E in BAA90980. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: EEBBB95B0A9863F4	FASTA	402	44,381
	10 20 30 40 50 60 MSAEGAEPGP GSGSGPGPGP LCPEHGQALS WFCGSERRPV CAACAGLGGR CRGHRIRRAE 70 80 90 100 110 120 ERAEELRNKI VDQCERLQLQ SAAITKYVAD VLPGKNQRAV SMASAARELV IQRLSLVRSL 130 140 150 160 170 180 CESEEQRLLE QVHGEEERAH QSILTQRVHW AEALQKLDTI RTGLVGMLTH LDDLQLIQKE 190 200 210 220 230 240 QEIFERTEEA EGILDPQESE MLNFNEKCTR SPLLTQLWAT AVLGSLSGTE DIRIDERTVS 250 260 270 280 290 300 PFLQLSDDRK TLTFSTKKSK ACADGPERFD HWPNALAATS FQNGLHAWMV NVQNSCAYKV 310 320 330 340 350 360 GVASGHLPRK GSGSDCRLGH NAFSWVFSRY DQEFRFSHNG QHEPLGLLRG PAQLGVVLDL 370 380 390 400 QVQELLFYEP ASGTVLCAHH VSFPGPLFPV FAVADQTISI VR
	Isoform 2 [UniParc]. Checksum: 39D43152A32707B0	FASTA	193	21,413
	10 20 30 40 50 60 MSAEGAEPGP GSGSGPGPGP LCPEHGQALS WFCGSERRPV CAACAGLGGR CRGHRIRRAE 70 80 90 100 110 120 ERAEELRNKI VDQCERLQLQ SAAITKYVAD VLPGKNQRAV SMASAARELV IQRLSLVRSL 130 140 150 160 170 180 CESEEQRLLE QVHGEEERAH QSILTQRVHW AEALQKLDTI RTGLVGMLTH LDDLQLIQKE 190 QEIFERHRGH TDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BSPRY, a novel protein of the Ro-Ret family." Schenker T., Trueb B. Biochim. Biophys. Acta 1493:255-258(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ILE-374. Tissue: Placenta.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-374. Tissue: Colon and Prostate.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ276691 mRNA. Translation: CAC09324.1. AL137066 Genomic DNA. Translation: CAH70097.1. CH471090 Genomic DNA. Translation: EAW87374.1. BC001477 mRNA. Translation: AAH01477.1. AK000157 mRNA. Translation: BAA90980.1. Different initiation. AK092607 mRNA. Translation: BAG52581.1.
IPI	IPI00185568. IPI00301791.
RefSeq	NP_060158.2. NM_017688.2.
UniGene	Hs.614517.
3D structure databases
ProteinModelPortal	Q5W0U4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5W0U4. 1 interaction.
STRING	9606.ENSP00000363298.
PTM databases
PhosphoSite	Q5W0U4.
Polymorphism databases
DMDM	74747982.
Proteomic databases
PaxDb	Q5W0U4.
PRIDE	Q5W0U4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000374183; ENSP00000363298; ENSG00000119411.
GeneID	54836.
KEGG	hsa:54836.
UCSC	uc004bhg.4. human. uc010muw.3. human.
Organism-specific databases
CTD	54836.
GeneCards	GC09P116111.
HGNC	HGNC:18232. BSPRY.
HPA	HPA042889.
neXtProt	NX_Q5W0U4.
PharmGKB	PA134979106.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264516.
HOGENOM	HOG000057290.
HOVERGEN	HBG080574.
InParanoid	Q5W0U4.
OMA	AQELLFY.
OrthoDB	EOG4RV2RX.
PhylomeDB	Q5W0U4.
Gene expression databases
Bgee	Q5W0U4.
CleanEx	HS_BSPRY.
Genevestigator	Q5W0U4.
GermOnline	ENSG00000119411. Homo sapiens.
Family and domain databases
InterPro	IPR001870. B30.2/SPRY. IPR003879. Butyrophylin. IPR008985. ConA-like_lec_gl_sf. IPR006574. PRY. IPR003877. SPRY_rcpt. IPR000315. Znf_B-box. [Graphical view]
Pfam	PF00622. SPRY. 1 hit. PF00643. zf-B_box. 1 hit. [Graphical view]
PRINTS	PR01407. BUTYPHLNCDUF.
SMART	SM00589. PRY. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS50188. B302_SPRY. 1 hit. PS50119. ZF_BBOX. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BSPRY. human.
GenomeRNAi	54836.
NextBio	57630.

Entry information

Entry name

BSPRY_HUMAN

Accession

Primary (citable) accession number: Q5W0U4
Secondary accession number(s): B3KS19 , Q96DJ2, Q9H4E4, Q9NXN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.