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Q5W0Q7

- USPL1_HUMAN

UniProt

Q5W0Q7 - USPL1_HUMAN

Protein

SUMO-specific isopeptidase USPL1

Gene

USPL1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms. May have non-catalytic functions in Cajal bodies organization and cell proliferation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei236 – 2361Nucleophile
    Active sitei456 – 4561Proton acceptorBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. SUMO binding Source: UniProtKB
    3. SUMO-specific isopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. Cajal body organization Source: UniProtKB
    2. cell proliferation Source: UniProtKB
    3. protein desumoylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Protein family/group databases

    MEROPSiC98.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SUMO-specific isopeptidase USPL1 (EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin-specific peptidase-like protein 1
    Gene namesi
    Name:USPL1
    Synonyms:C13orf22, D13S106
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:20294. USPL1.

    Subcellular locationi

    NucleusCajal body 1 Publication

    GO - Cellular componenti

    1. Cajal body Source: UniProtKB
    2. extracellular space Source: UniProt

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi229 – 2291W → L: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
    Mutagenesisi236 – 2361C → S: Abolishes the SUMO-specific isopeptidase activity. 1 Publication
    Mutagenesisi237 – 2371W → F: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
    Mutagenesisi340 – 3412LL → AA: Altered SUMO-binding and SUMO-specific isopeptidase activity.
    Mutagenesisi421 – 4211H → A: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
    Mutagenesisi494 – 4952IV → AA: Loss of SUMO-binding and catalytic activity.

    Organism-specific databases

    PharmGKBiPA134872379.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10921092SUMO-specific isopeptidase USPL1PRO_0000279526Add
    BLAST

    Proteomic databases

    PaxDbiQ5W0Q7.
    PRIDEiQ5W0Q7.

    PTM databases

    PhosphoSiteiQ5W0Q7.

    Expressioni

    Gene expression databases

    BgeeiQ5W0Q7.
    CleanExiHS_USPL1.
    GenevestigatoriQ5W0Q7.

    Organism-specific databases

    HPAiHPA039342.
    HPA040172.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SUMO1P631655EBI-2513899,EBI-80140
    SUMO2P619562EBI-2513899,EBI-473220
    SUMO3P5585413EBI-2513899,EBI-474067

    Protein-protein interaction databases

    BioGridi115503. 18 interactions.
    DIPiDIP-47278N.
    IntActiQ5W0Q7. 17 interactions.
    MINTiMINT-8403754.
    STRINGi9606.ENSP00000255304.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5W0Q7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini227 – 500274USPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni236 – 495260SUMO-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG39418.
    HOGENOMiHOG000154801.
    HOVERGENiHBG094141.
    InParanoidiQ5W0Q7.
    OMAiGPGTDIG.
    OrthoDBiEOG715Q39.
    PhylomeDBiQ5W0Q7.
    TreeFamiTF350670.

    Family and domain databases

    InterProiIPR029388. DUF4650.
    IPR028890. Peptidase_C98.
    IPR028891. SUMO_USPL1.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PANTHERiPTHR15294. PTHR15294. 1 hit.
    PfamiPF15509. DUF4650. 1 hit.
    PF15499. Peptidase_C98. 1 hit.
    [Graphical view]
    PROSITEiPS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5W0Q7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMDSPKIGNG LPVIGPGTDI GISSLHMVGY LGKNFDSAKV PSDEYCPACR     50
    EKGKLKALKT YRISFQESIF LCEDLQCIYP LGSKSLNNLI SPDLEECHTP 100
    HKPQKRKSLE SSYKDSLLLA NSKKTRNYIA IDGGKVLNSK HNGEVYDETS 150
    SNLPDSSGQQ NPIRTADSLE RNEILEADTV DMATTKDPAT VDVSGTGRPS 200
    PQNEGCTSKL EMPLESKCTS FPQALCVQWK NAYALCWLDC ILSALVHSEE 250
    LKNTVTGLCS KEESIFWRLL TKYNQANTLL YTSQLSGVKD GDCKKLTSEI 300
    FAEIETCLNE VRDEIFISLQ PQLRCTLGDM ESPVFAFPLL LKLETHIEKL 350
    FLYSFSWDFE CSQCGHQYQN RHMKSLVTFT NVIPEWHPLN AAHFGPCNNC 400
    NSKSQIRKMV LEKVSPIFML HFVEGLPQND LQHYAFHFEG CLYQITSVIQ 450
    YRANNHFITW ILDADGSWLE CDDLKGPCSE RHKKFEVPAS EIHIVIWERK 500
    ISQVTDKEAA CLPLKKTNDQ HALSNEKPVS LTSCSVGDAA SAETASVTHP 550
    KDISVAPRTL SQDTAVTHGD HLLSGPKGLV DNILPLTLEE TIQKTASVSQ 600
    LNSEAFLLEN KPVAENTGIL KTNTLLSQES LMASSVSAPC NEKLIQDQFV 650
    DISFPSQVVN TNMQSVQLNT EDTVNTKSVN NTDATGLIQG VKSVEIEKDA 700
    QLKQFLTPKT EQLKPERVTS QVSNLKKKET TADSQTTTSK SLQNQSLKEN 750
    QKKPFVGSWV KGLISRGASF MPLCVSAHNR NTITDLQPSV KGVNNFGGFK 800
    TKGINQKASH VSKKARKSAS KPPPISKPPA GPPSSNGTAA HPHAHAASEV 850
    LEKSGSTSCG AQLNHSSYGN GISSANHEDL VEGQIHKLRL KLRKKLKAEK 900
    KKLAALMSSP QSRTVRSENL EQVPQDGSPN DCESIEDLLN ELPYPIDIAS 950
    ESACTTVPGV SLYSSQTHEE ILAELLSPTP VSTELSENGE GDFRYLGMGD 1000
    SHIPPPVPSE FNDVSQNTHL RQDHNYCSPT KKNPCEVQPD SLTNNACVRT 1050
    LNLESPMKTD IFDEFFSSSA LNALANDTLD LPHFDEYLFE NY 1092
    Length:1,092
    Mass (Da):120,440
    Last modified:December 7, 2004 - v1
    Checksum:iFBE4626FC311E706
    GO
    Isoform 2 (identifier: Q5W0Q7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-329: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:763
    Mass (Da):84,094
    Checksum:i3E1D7BDC10690EDA
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti173 – 1731E → G.1 Publication
    Corresponds to variant rs17853512 [ dbSNP | Ensembl ].
    VAR_030916
    Natural varianti384 – 3841P → S.
    Corresponds to variant rs3742303 [ dbSNP | Ensembl ].
    VAR_030917
    Natural varianti522 – 5221A → P.
    Corresponds to variant rs17609459 [ dbSNP | Ensembl ].
    VAR_030918
    Natural varianti531 – 5311L → S.1 Publication
    Corresponds to variant rs7984952 [ dbSNP | Ensembl ].
    VAR_030919
    Natural varianti583 – 5831I → V.
    Corresponds to variant rs41412648 [ dbSNP | Ensembl ].
    VAR_051542
    Natural varianti739 – 7391S → C.
    Corresponds to variant rs9578190 [ dbSNP | Ensembl ].
    VAR_030920
    Natural varianti786 – 7861L → I.
    Corresponds to variant rs35371042 [ dbSNP | Ensembl ].
    VAR_051543
    Natural varianti950 – 9501S → N.1 Publication
    Corresponds to variant rs3742302 [ dbSNP | Ensembl ].
    VAR_030921
    Natural varianti1043 – 10431T → S.1 Publication
    Corresponds to variant rs17857086 [ dbSNP | Ensembl ].
    VAR_030922

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 329329Missing in isoform 2. 1 PublicationVSP_023479Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59131 mRNA. Translation: CAA41848.1.
    CR627370 mRNA. Translation: CAH10469.1.
    AL138681 Genomic DNA. Translation: CAH70264.1.
    BC036027 mRNA. Translation: AAH36027.1.
    BC038103 mRNA. Translation: AAH38103.1.
    CCDSiCCDS9336.1. [Q5W0Q7-1]
    PIRiI37273.
    RefSeqiNP_005791.3. NM_005800.4. [Q5W0Q7-1]
    XP_005266270.1. XM_005266213.1. [Q5W0Q7-1]
    UniGeneiHs.533831.

    Genome annotation databases

    EnsembliENST00000255304; ENSP00000255304; ENSG00000132952. [Q5W0Q7-1]
    GeneIDi10208.
    KEGGihsa:10208.
    UCSCiuc001utc.2. human. [Q5W0Q7-1]

    Polymorphism databases

    DMDMi74747969.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59131 mRNA. Translation: CAA41848.1 .
    CR627370 mRNA. Translation: CAH10469.1 .
    AL138681 Genomic DNA. Translation: CAH70264.1 .
    BC036027 mRNA. Translation: AAH36027.1 .
    BC038103 mRNA. Translation: AAH38103.1 .
    CCDSi CCDS9336.1. [Q5W0Q7-1 ]
    PIRi I37273.
    RefSeqi NP_005791.3. NM_005800.4. [Q5W0Q7-1 ]
    XP_005266270.1. XM_005266213.1. [Q5W0Q7-1 ]
    UniGenei Hs.533831.

    3D structure databases

    ProteinModelPortali Q5W0Q7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115503. 18 interactions.
    DIPi DIP-47278N.
    IntActi Q5W0Q7. 17 interactions.
    MINTi MINT-8403754.
    STRINGi 9606.ENSP00000255304.

    Protein family/group databases

    MEROPSi C98.001.

    PTM databases

    PhosphoSitei Q5W0Q7.

    Polymorphism databases

    DMDMi 74747969.

    Proteomic databases

    PaxDbi Q5W0Q7.
    PRIDEi Q5W0Q7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000255304 ; ENSP00000255304 ; ENSG00000132952 . [Q5W0Q7-1 ]
    GeneIDi 10208.
    KEGGi hsa:10208.
    UCSCi uc001utc.2. human. [Q5W0Q7-1 ]

    Organism-specific databases

    CTDi 10208.
    GeneCardsi GC13P031191.
    H-InvDB HIX0023052.
    HGNCi HGNC:20294. USPL1.
    HPAi HPA039342.
    HPA040172.
    neXtProti NX_Q5W0Q7.
    PharmGKBi PA134872379.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG39418.
    HOGENOMi HOG000154801.
    HOVERGENi HBG094141.
    InParanoidi Q5W0Q7.
    OMAi GPGTDIG.
    OrthoDBi EOG715Q39.
    PhylomeDBi Q5W0Q7.
    TreeFami TF350670.

    Miscellaneous databases

    GenomeRNAii 10208.
    NextBioi 38648.
    PROi Q5W0Q7.

    Gene expression databases

    Bgeei Q5W0Q7.
    CleanExi HS_USPL1.
    Genevestigatori Q5W0Q7.

    Family and domain databases

    InterProi IPR029388. DUF4650.
    IPR028890. Peptidase_C98.
    IPR028891. SUMO_USPL1.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    PANTHERi PTHR15294. PTHR15294. 1 hit.
    Pfami PF15509. DUF4650. 1 hit.
    PF15499. Peptidase_C98. 1 hit.
    [Graphical view ]
    PROSITEi PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A unique intronless gene or gene segment on chromosome 13 specifying a highly charged amino acid sequence."
      Wilton A.N., Zehavi-Feferman T., Fleming J., Baker E., Chen L.Z., Cooper D.W.
      Cytogenet. Cell Genet. 58:1985-1985(1991)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Melanoma.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Semen.
    3. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLY-173; SER-531; ASN-950 AND SER-1043.
      Tissue: Testis.
    5. "Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions."
      Schulz S., Chachami G., Kozaczkiewicz L., Winter U., Stankovic-Valentin N., Haas P., Hofmann K., Urlaub H., Ovaa H., Wittbrodt J., Meulmeester E., Melchior F.
      EMBO Rep. 13:930-938(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PROTEIN DESUMOYLATION, NON-CATALYTIC FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-229; CYS-236; TRP-237; HIS-421; 340-LEU-LEU-341 AND 494-ILE-VAL-495.

    Entry informationi

    Entry nameiUSPL1_HUMAN
    AccessioniPrimary (citable) accession number: Q5W0Q7
    Secondary accession number(s): Q14109
    , Q6AI45, Q8IY30, Q8IYE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3