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Q5W0Q7 (USPL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SUMO-specific isopeptidase USPL1

EC=3.4.22.-
Alternative name(s):
Ubiquitin-specific peptidase-like protein 1
Gene names
Name:USPL1
Synonyms:C13orf22, D13S106
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1092 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms. May have non-catalytic functions in Cajal bodies organization and cell proliferation. Ref.5

Subcellular location

NucleusCajal body Ref.5.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5W0Q7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5W0Q7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-329: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10921092SUMO-specific isopeptidase USPL1
PRO_0000279526

Regions

Domain227 – 500274USP
Region236 – 495260SUMO-binding

Sites

Active site2361Nucleophile
Active site4561Proton acceptor By similarity

Natural variations

Alternative sequence1 – 329329Missing in isoform 2.
VSP_023479
Natural variant1731E → G. Ref.4
Corresponds to variant rs17853512 [ dbSNP | Ensembl ].
VAR_030916
Natural variant3841P → S.
Corresponds to variant rs3742303 [ dbSNP | Ensembl ].
VAR_030917
Natural variant5221A → P.
Corresponds to variant rs17609459 [ dbSNP | Ensembl ].
VAR_030918
Natural variant5311L → S. Ref.4
Corresponds to variant rs7984952 [ dbSNP | Ensembl ].
VAR_030919
Natural variant5831I → V.
Corresponds to variant rs41412648 [ dbSNP | Ensembl ].
VAR_051542
Natural variant7391S → C.
Corresponds to variant rs9578190 [ dbSNP | Ensembl ].
VAR_030920
Natural variant7861L → I.
Corresponds to variant rs35371042 [ dbSNP | Ensembl ].
VAR_051543
Natural variant9501S → N. Ref.4
Corresponds to variant rs3742302 [ dbSNP | Ensembl ].
VAR_030921
Natural variant10431T → S. Ref.4
Corresponds to variant rs17857086 [ dbSNP | Ensembl ].
VAR_030922

Experimental info

Mutagenesis2291W → L: Altered SUMO-binding and SUMO-specific isopeptidase activity. Ref.5
Mutagenesis2361C → S: Abolishes the SUMO-specific isopeptidase activity. Ref.5
Mutagenesis2371W → F: Altered SUMO-binding and SUMO-specific isopeptidase activity. Ref.5
Mutagenesis340 – 3412LL → AA: Altered SUMO-binding and SUMO-specific isopeptidase activity.
Mutagenesis4211H → A: Altered SUMO-binding and SUMO-specific isopeptidase activity. Ref.5
Mutagenesis494 – 4952IV → AA: Loss of SUMO-binding and catalytic activity.

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: FBE4626FC311E706

FASTA1,092120,440
        10         20         30         40         50         60 
MMDSPKIGNG LPVIGPGTDI GISSLHMVGY LGKNFDSAKV PSDEYCPACR EKGKLKALKT 

        70         80         90        100        110        120 
YRISFQESIF LCEDLQCIYP LGSKSLNNLI SPDLEECHTP HKPQKRKSLE SSYKDSLLLA 

       130        140        150        160        170        180 
NSKKTRNYIA IDGGKVLNSK HNGEVYDETS SNLPDSSGQQ NPIRTADSLE RNEILEADTV 

       190        200        210        220        230        240 
DMATTKDPAT VDVSGTGRPS PQNEGCTSKL EMPLESKCTS FPQALCVQWK NAYALCWLDC 

       250        260        270        280        290        300 
ILSALVHSEE LKNTVTGLCS KEESIFWRLL TKYNQANTLL YTSQLSGVKD GDCKKLTSEI 

       310        320        330        340        350        360 
FAEIETCLNE VRDEIFISLQ PQLRCTLGDM ESPVFAFPLL LKLETHIEKL FLYSFSWDFE 

       370        380        390        400        410        420 
CSQCGHQYQN RHMKSLVTFT NVIPEWHPLN AAHFGPCNNC NSKSQIRKMV LEKVSPIFML 

       430        440        450        460        470        480 
HFVEGLPQND LQHYAFHFEG CLYQITSVIQ YRANNHFITW ILDADGSWLE CDDLKGPCSE 

       490        500        510        520        530        540 
RHKKFEVPAS EIHIVIWERK ISQVTDKEAA CLPLKKTNDQ HALSNEKPVS LTSCSVGDAA 

       550        560        570        580        590        600 
SAETASVTHP KDISVAPRTL SQDTAVTHGD HLLSGPKGLV DNILPLTLEE TIQKTASVSQ 

       610        620        630        640        650        660 
LNSEAFLLEN KPVAENTGIL KTNTLLSQES LMASSVSAPC NEKLIQDQFV DISFPSQVVN 

       670        680        690        700        710        720 
TNMQSVQLNT EDTVNTKSVN NTDATGLIQG VKSVEIEKDA QLKQFLTPKT EQLKPERVTS 

       730        740        750        760        770        780 
QVSNLKKKET TADSQTTTSK SLQNQSLKEN QKKPFVGSWV KGLISRGASF MPLCVSAHNR 

       790        800        810        820        830        840 
NTITDLQPSV KGVNNFGGFK TKGINQKASH VSKKARKSAS KPPPISKPPA GPPSSNGTAA 

       850        860        870        880        890        900 
HPHAHAASEV LEKSGSTSCG AQLNHSSYGN GISSANHEDL VEGQIHKLRL KLRKKLKAEK 

       910        920        930        940        950        960 
KKLAALMSSP QSRTVRSENL EQVPQDGSPN DCESIEDLLN ELPYPIDIAS ESACTTVPGV 

       970        980        990       1000       1010       1020 
SLYSSQTHEE ILAELLSPTP VSTELSENGE GDFRYLGMGD SHIPPPVPSE FNDVSQNTHL 

      1030       1040       1050       1060       1070       1080 
RQDHNYCSPT KKNPCEVQPD SLTNNACVRT LNLESPMKTD IFDEFFSSSA LNALANDTLD 

      1090 
LPHFDEYLFE NY 

« Hide

Isoform 2 [UniParc].

Checksum: 3E1D7BDC10690EDA
Show »

FASTA76384,094

References

« Hide 'large scale' references
[1]"A unique intronless gene or gene segment on chromosome 13 specifying a highly charged amino acid sequence."
Wilton A.N., Zehavi-Feferman T., Fleming J., Baker E., Chen L.Z., Cooper D.W.
Cytogenet. Cell Genet. 58:1985-1985(1991)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Melanoma.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Semen.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLY-173; SER-531; ASN-950 AND SER-1043.
Tissue: Testis.
[5]"Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions."
Schulz S., Chachami G., Kozaczkiewicz L., Winter U., Stankovic-Valentin N., Haas P., Hofmann K., Urlaub H., Ovaa H., Wittbrodt J., Meulmeester E., Melchior F.
EMBO Rep. 13:930-938(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PROTEIN DESUMOYLATION, NON-CATALYTIC FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-229; CYS-236; TRP-237; HIS-421; 340-LEU-LEU-341 AND 494-ILE-VAL-495.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59131 mRNA. Translation: CAA41848.1.
CR627370 mRNA. Translation: CAH10469.1.
AL138681 Genomic DNA. Translation: CAH70264.1.
BC036027 mRNA. Translation: AAH36027.1.
BC038103 mRNA. Translation: AAH38103.1.
CCDSCCDS9336.1. [Q5W0Q7-1]
PIRI37273.
RefSeqNP_005791.3. NM_005800.4. [Q5W0Q7-1]
XP_005266270.1. XM_005266213.1. [Q5W0Q7-1]
UniGeneHs.533831.

3D structure databases

ProteinModelPortalQ5W0Q7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115503. 18 interactions.
DIPDIP-47278N.
IntActQ5W0Q7. 16 interactions.
MINTMINT-8403754.
STRING9606.ENSP00000255304.

Protein family/group databases

MEROPSC98.001.

PTM databases

PhosphoSiteQ5W0Q7.

Polymorphism databases

DMDM74747969.

Proteomic databases

PaxDbQ5W0Q7.
PRIDEQ5W0Q7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000255304; ENSP00000255304; ENSG00000132952. [Q5W0Q7-1]
GeneID10208.
KEGGhsa:10208.
UCSCuc001utc.2. human. [Q5W0Q7-1]

Organism-specific databases

CTD10208.
GeneCardsGC13P031191.
H-InvDBHIX0023052.
HGNCHGNC:20294. USPL1.
HPAHPA039342.
HPA040172.
neXtProtNX_Q5W0Q7.
PharmGKBPA134872379.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39418.
HOGENOMHOG000154801.
HOVERGENHBG094141.
InParanoidQ5W0Q7.
OMAGPGTDIG.
OrthoDBEOG715Q39.
PhylomeDBQ5W0Q7.
TreeFamTF350670.

Gene expression databases

BgeeQ5W0Q7.
CleanExHS_USPL1.
GenevestigatorQ5W0Q7.

Family and domain databases

InterProIPR029388. DUF4650.
IPR028890. Peptidase_C98.
IPR028891. SUMO_USPL1.
IPR028889. UCH/PAN2.
[Graphical view]
PANTHERPTHR15294. PTHR15294. 1 hit.
PfamPF15509. DUF4650. 1 hit.
PF15499. Peptidase_C98. 1 hit.
[Graphical view]
PROSITEPS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10208.
NextBio38648.
PROQ5W0Q7.

Entry information

Entry nameUSPL1_HUMAN
AccessionPrimary (citable) accession number: Q5W0Q7
Secondary accession number(s): Q14109 expand/collapse secondary AC list , Q6AI45, Q8IY30, Q8IYE8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM