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Protein

SUMO-specific isopeptidase USPL1

Gene

USPL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms. May have non-catalytic functions in Cajal bodies organization and cell proliferation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei236 – 2361Nucleophile
Active sitei456 – 4561Proton acceptorBy similarity

GO - Molecular functioni

  1. SUMO binding Source: UniProtKB
  2. SUMO-specific isopeptidase activity Source: UniProtKB

GO - Biological processi

  1. Cajal body organization Source: UniProtKB
  2. cell proliferation Source: UniProtKB
  3. protein desumoylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC98.001.

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-specific isopeptidase USPL1 (EC:3.4.22.-)
Alternative name(s):
Ubiquitin-specific peptidase-like protein 1
Gene namesi
Name:USPL1
Synonyms:C13orf22, D13S106
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20294. USPL1.

Subcellular locationi

  1. NucleusCajal body 1 Publication

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi229 – 2291W → L: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
Mutagenesisi236 – 2361C → S: Abolishes the SUMO-specific isopeptidase activity. 1 Publication
Mutagenesisi237 – 2371W → F: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
Mutagenesisi340 – 3412LL → AA: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
Mutagenesisi421 – 4211H → A: Altered SUMO-binding and SUMO-specific isopeptidase activity. 1 Publication
Mutagenesisi494 – 4952IV → AA: Loss of SUMO-binding and catalytic activity. 1 Publication

Organism-specific databases

PharmGKBiPA134872379.

Polymorphism and mutation databases

BioMutaiUSPL1.
DMDMi74747969.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092SUMO-specific isopeptidase USPL1PRO_0000279526Add
BLAST

Proteomic databases

MaxQBiQ5W0Q7.
PaxDbiQ5W0Q7.
PRIDEiQ5W0Q7.

PTM databases

PhosphoSiteiQ5W0Q7.

Expressioni

Gene expression databases

BgeeiQ5W0Q7.
CleanExiHS_USPL1.
GenevestigatoriQ5W0Q7.

Organism-specific databases

HPAiHPA039342.
HPA040172.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SUMO1P631655EBI-2513899,EBI-80140
SUMO2P619562EBI-2513899,EBI-473220
SUMO3P5585416EBI-2513899,EBI-474067

Protein-protein interaction databases

BioGridi115503. 25 interactions.
DIPiDIP-47278N.
IntActiQ5W0Q7. 17 interactions.
MINTiMINT-8403754.
STRINGi9606.ENSP00000255304.

Structurei

3D structure databases

ProteinModelPortaliQ5W0Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini227 – 500274USPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 495260SUMO-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiNOG39418.
GeneTreeiENSGT00390000002316.
HOGENOMiHOG000154801.
HOVERGENiHBG094141.
InParanoidiQ5W0Q7.
OMAiGPGTDIG.
OrthoDBiEOG715Q39.
PhylomeDBiQ5W0Q7.
TreeFamiTF350670.

Family and domain databases

InterProiIPR029388. DUF4650.
IPR028890. Peptidase_C98.
IPR028891. SUMO_USPL1.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR15294. PTHR15294. 1 hit.
PfamiPF15509. DUF4650. 1 hit.
PF15499. Peptidase_C98. 1 hit.
[Graphical view]
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5W0Q7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMDSPKIGNG LPVIGPGTDI GISSLHMVGY LGKNFDSAKV PSDEYCPACR
60 70 80 90 100
EKGKLKALKT YRISFQESIF LCEDLQCIYP LGSKSLNNLI SPDLEECHTP
110 120 130 140 150
HKPQKRKSLE SSYKDSLLLA NSKKTRNYIA IDGGKVLNSK HNGEVYDETS
160 170 180 190 200
SNLPDSSGQQ NPIRTADSLE RNEILEADTV DMATTKDPAT VDVSGTGRPS
210 220 230 240 250
PQNEGCTSKL EMPLESKCTS FPQALCVQWK NAYALCWLDC ILSALVHSEE
260 270 280 290 300
LKNTVTGLCS KEESIFWRLL TKYNQANTLL YTSQLSGVKD GDCKKLTSEI
310 320 330 340 350
FAEIETCLNE VRDEIFISLQ PQLRCTLGDM ESPVFAFPLL LKLETHIEKL
360 370 380 390 400
FLYSFSWDFE CSQCGHQYQN RHMKSLVTFT NVIPEWHPLN AAHFGPCNNC
410 420 430 440 450
NSKSQIRKMV LEKVSPIFML HFVEGLPQND LQHYAFHFEG CLYQITSVIQ
460 470 480 490 500
YRANNHFITW ILDADGSWLE CDDLKGPCSE RHKKFEVPAS EIHIVIWERK
510 520 530 540 550
ISQVTDKEAA CLPLKKTNDQ HALSNEKPVS LTSCSVGDAA SAETASVTHP
560 570 580 590 600
KDISVAPRTL SQDTAVTHGD HLLSGPKGLV DNILPLTLEE TIQKTASVSQ
610 620 630 640 650
LNSEAFLLEN KPVAENTGIL KTNTLLSQES LMASSVSAPC NEKLIQDQFV
660 670 680 690 700
DISFPSQVVN TNMQSVQLNT EDTVNTKSVN NTDATGLIQG VKSVEIEKDA
710 720 730 740 750
QLKQFLTPKT EQLKPERVTS QVSNLKKKET TADSQTTTSK SLQNQSLKEN
760 770 780 790 800
QKKPFVGSWV KGLISRGASF MPLCVSAHNR NTITDLQPSV KGVNNFGGFK
810 820 830 840 850
TKGINQKASH VSKKARKSAS KPPPISKPPA GPPSSNGTAA HPHAHAASEV
860 870 880 890 900
LEKSGSTSCG AQLNHSSYGN GISSANHEDL VEGQIHKLRL KLRKKLKAEK
910 920 930 940 950
KKLAALMSSP QSRTVRSENL EQVPQDGSPN DCESIEDLLN ELPYPIDIAS
960 970 980 990 1000
ESACTTVPGV SLYSSQTHEE ILAELLSPTP VSTELSENGE GDFRYLGMGD
1010 1020 1030 1040 1050
SHIPPPVPSE FNDVSQNTHL RQDHNYCSPT KKNPCEVQPD SLTNNACVRT
1060 1070 1080 1090
LNLESPMKTD IFDEFFSSSA LNALANDTLD LPHFDEYLFE NY
Length:1,092
Mass (Da):120,440
Last modified:December 7, 2004 - v1
Checksum:iFBE4626FC311E706
GO
Isoform 2 (identifier: Q5W0Q7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-329: Missing.

Note: No experimental confirmation available.

Show »
Length:763
Mass (Da):84,094
Checksum:i3E1D7BDC10690EDA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti173 – 1731E → G.1 Publication
Corresponds to variant rs17853512 [ dbSNP | Ensembl ].
VAR_030916
Natural varianti384 – 3841P → S.
Corresponds to variant rs3742303 [ dbSNP | Ensembl ].
VAR_030917
Natural varianti522 – 5221A → P.
Corresponds to variant rs17609459 [ dbSNP | Ensembl ].
VAR_030918
Natural varianti531 – 5311L → S.1 Publication
Corresponds to variant rs7984952 [ dbSNP | Ensembl ].
VAR_030919
Natural varianti583 – 5831I → V.
Corresponds to variant rs41412648 [ dbSNP | Ensembl ].
VAR_051542
Natural varianti739 – 7391S → C.
Corresponds to variant rs9578190 [ dbSNP | Ensembl ].
VAR_030920
Natural varianti786 – 7861L → I.
Corresponds to variant rs35371042 [ dbSNP | Ensembl ].
VAR_051543
Natural varianti950 – 9501S → N.1 Publication
Corresponds to variant rs3742302 [ dbSNP | Ensembl ].
VAR_030921
Natural varianti1043 – 10431T → S.1 Publication
Corresponds to variant rs17857086 [ dbSNP | Ensembl ].
VAR_030922

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 329329Missing in isoform 2. 1 PublicationVSP_023479Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59131 mRNA. Translation: CAA41848.1.
CR627370 mRNA. Translation: CAH10469.1.
AL138681 Genomic DNA. Translation: CAH70264.1.
BC036027 mRNA. Translation: AAH36027.1.
BC038103 mRNA. Translation: AAH38103.1.
CCDSiCCDS9336.1. [Q5W0Q7-1]
PIRiI37273.
RefSeqiNP_005791.3. NM_005800.4. [Q5W0Q7-1]
XP_005266270.1. XM_005266213.2. [Q5W0Q7-1]
UniGeneiHs.533831.

Genome annotation databases

EnsembliENST00000255304; ENSP00000255304; ENSG00000132952. [Q5W0Q7-1]
ENST00000614860; ENSP00000480656; ENSG00000132952. [Q5W0Q7-2]
GeneIDi10208.
KEGGihsa:10208.
UCSCiuc001utc.2. human. [Q5W0Q7-1]

Polymorphism and mutation databases

BioMutaiUSPL1.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59131 mRNA. Translation: CAA41848.1.
CR627370 mRNA. Translation: CAH10469.1.
AL138681 Genomic DNA. Translation: CAH70264.1.
BC036027 mRNA. Translation: AAH36027.1.
BC038103 mRNA. Translation: AAH38103.1.
CCDSiCCDS9336.1. [Q5W0Q7-1]
PIRiI37273.
RefSeqiNP_005791.3. NM_005800.4. [Q5W0Q7-1]
XP_005266270.1. XM_005266213.2. [Q5W0Q7-1]
UniGeneiHs.533831.

3D structure databases

ProteinModelPortaliQ5W0Q7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115503. 25 interactions.
DIPiDIP-47278N.
IntActiQ5W0Q7. 17 interactions.
MINTiMINT-8403754.
STRINGi9606.ENSP00000255304.

Protein family/group databases

MEROPSiC98.001.

PTM databases

PhosphoSiteiQ5W0Q7.

Polymorphism and mutation databases

BioMutaiUSPL1.
DMDMi74747969.

Proteomic databases

MaxQBiQ5W0Q7.
PaxDbiQ5W0Q7.
PRIDEiQ5W0Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255304; ENSP00000255304; ENSG00000132952. [Q5W0Q7-1]
ENST00000614860; ENSP00000480656; ENSG00000132952. [Q5W0Q7-2]
GeneIDi10208.
KEGGihsa:10208.
UCSCiuc001utc.2. human. [Q5W0Q7-1]

Organism-specific databases

CTDi10208.
GeneCardsiGC13P031191.
H-InvDBHIX0023052.
HGNCiHGNC:20294. USPL1.
HPAiHPA039342.
HPA040172.
neXtProtiNX_Q5W0Q7.
PharmGKBiPA134872379.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39418.
GeneTreeiENSGT00390000002316.
HOGENOMiHOG000154801.
HOVERGENiHBG094141.
InParanoidiQ5W0Q7.
OMAiGPGTDIG.
OrthoDBiEOG715Q39.
PhylomeDBiQ5W0Q7.
TreeFamiTF350670.

Miscellaneous databases

GenomeRNAii10208.
NextBioi38648.
PROiQ5W0Q7.

Gene expression databases

BgeeiQ5W0Q7.
CleanExiHS_USPL1.
GenevestigatoriQ5W0Q7.

Family and domain databases

InterProiIPR029388. DUF4650.
IPR028890. Peptidase_C98.
IPR028891. SUMO_USPL1.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR15294. PTHR15294. 1 hit.
PfamiPF15509. DUF4650. 1 hit.
PF15499. Peptidase_C98. 1 hit.
[Graphical view]
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A unique intronless gene or gene segment on chromosome 13 specifying a highly charged amino acid sequence."
    Wilton A.N., Zehavi-Feferman T., Fleming J., Baker E., Chen L.Z., Cooper D.W.
    Cytogenet. Cell Genet. 58:1985-1985(1991)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Melanoma.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Semen.
  3. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLY-173; SER-531; ASN-950 AND SER-1043.
    Tissue: Testis.
  5. "Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions."
    Schulz S., Chachami G., Kozaczkiewicz L., Winter U., Stankovic-Valentin N., Haas P., Hofmann K., Urlaub H., Ovaa H., Wittbrodt J., Meulmeester E., Melchior F.
    EMBO Rep. 13:930-938(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PROTEIN DESUMOYLATION, NON-CATALYTIC FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-229; CYS-236; TRP-237; HIS-421; 340-LEU-LEU-341 AND 494-ILE-VAL-495.

Entry informationi

Entry nameiUSPL1_HUMAN
AccessioniPrimary (citable) accession number: Q5W0Q7
Secondary accession number(s): Q14109
, Q6AI45, Q8IY30, Q8IYE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 7, 2004
Last modified: April 29, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.