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Protein

Phosphatidate phosphatase PPAPDC1A

Gene

PPAPDC1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol.1 Publication

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=104 µM for diacylglycerol pyrophosphate1 Publication
  2. KM=506 µM for phosphatidate1 Publication
  3. KM=580 µM for lysophosphatidate1 Publication

GO - Molecular functioni

  1. phosphatidate phosphatase activity Source: UniProtKB

GO - Biological processi

  1. Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  2. innate immune response Source: Reactome
  3. phospholipid dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.3.4. 2681.
3.1.3.81. 2681.
ReactomeiREACT_160158. Role of phospholipids in phagocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate phosphatase PPAPDC1A (EC:3.1.3.4)
Alternative name(s):
Phosphatidic acid phosphatase type 2 domain-containing protein 1A
Gene namesi
Name:PPAPDC1A
Synonyms:DPPL2, PPAPDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23531. PPAPDC1A.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence AnalysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091R → A: Loss of phosphatase activity; when associated with A-146 and with A-202. 1 Publication
Mutagenesisi146 – 1461H → A: Loss of phosphatase activity; when associated with A-109 and with A-202. 1 Publication
Mutagenesisi202 – 2021H → A: Loss of phosphatase activity; when associated with A-109 and with A-146. 1 Publication

Organism-specific databases

PharmGKBiPA134907381.

Polymorphism and mutation databases

BioMutaiPPAPDC1A.
DMDMi147721098.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Phosphatidate phosphatase PPAPDC1APRO_0000286943Add
BLAST

Proteomic databases

PaxDbiQ5VZY2.
PRIDEiQ5VZY2.

PTM databases

DEPODiQ5VZY2.
PhosphoSiteiQ5VZY2.

Expressioni

Tissue specificityi

Expressed mainly to the brain, kidney and testis, and to a lesser extent the bone marrow, thymus, prostate, liver and uterus.1 Publication

Gene expression databases

BgeeiQ5VZY2.
CleanExiHS_PPAPDC1A.
ExpressionAtlasiQ5VZY2. baseline and differential.
GenevestigatoriQ5VZY2.

Organism-specific databases

HPAiHPA045188.

Interactioni

Protein-protein interaction databases

BioGridi128189. 1 interaction.
STRINGi9606.ENSP00000381302.

Structurei

3D structure databases

ProteinModelPortaliQ5VZY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 1109Phosphatase sequence motif I
Regioni143 – 1464Phosphatase sequence motif II
Regioni195 – 20511Phosphatase sequence motif IIIAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0671.
GeneTreeiENSGT00510000046723.
HOGENOMiHOG000215098.
HOVERGENiHBG056764.
InParanoidiQ5VZY2.
OMAiFAVICVY.
OrthoDBiEOG79CZ0D.
PhylomeDBiQ5VZY2.
TreeFamiTF323722.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
IPR028668. PPAPDC1A/PPAPDC1B.
[Graphical view]
PANTHERiPTHR10165:SF90. PTHR10165:SF90. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VZY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRELAIEIGV RALLFGVFVF TEFLDPFQRV IQPEEIWLYK NPLVQSDNIP
60 70 80 90 100
TRLMFAISFL TPLAVICVVK IIRRTDKTEI KEAFLAVSLA LALNGVCTNT
110 120 130 140 150
IKLIVGRPRP DFFYRCFPDG VMNSEMHCTG DPDLVSEGRK SFPSIHSSFA
160 170 180 190 200
FSGLGFTTFY LAGKLHCFTE SGRGKSWRLC AAILPLYCAM MIALSRMCDY
210 220 230 240 250
KHHWQDSFVG GVIGLIFAYI CYRQHYPPLA NTACHKPYVS LRVPASLKKE
260 270
ERPTADSAPS LPLEGITEGP V
Length:271
Mass (Da):30,395
Last modified:May 15, 2007 - v2
Checksum:iC534549E44DDB9A1
GO
Isoform 2 (identifier: Q5VZY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     86-149: AVSLALALNGVCTNTIKLIVGRPRPDFFYRCFPDGVMNSEMHCTGDPDLVSEGRKSFPSIHSSF → V

Note: No experimental confirmation available.

Show »
Length:208
Mass (Da):23,509
Checksum:i9C4052029FE63D0F
GO
Isoform 3 (identifier: Q5VZY2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-189: Missing.

Note: No experimental confirmation available.

Show »
Length:82
Mass (Da):9,175
Checksum:iA228474E1B007DBA
GO
Isoform 4 (identifier: Q5VZY2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-96: AISFLTPLAV...VSLALALNGV → IPLWVESSAS...PTLVCESQPH
     97-271: Missing.

Note: No experimental confirmation available.

Show »
Length:96
Mass (Da):11,089
Checksum:iDE39DF5B00D26259
GO

Sequence cautioni

The sequence CAH70333.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 189189Missing in isoform 3. 1 PublicationVSP_025237Add
BLAST
Alternative sequencei56 – 9641AISFL…ALNGV → IPLWVESSASFLHTFATDST ILLWPTQLAINPTLVCESQP H in isoform 4. 1 PublicationVSP_025239Add
BLAST
Alternative sequencei86 – 14964AVSLA…IHSSF → V in isoform 2. 1 PublicationVSP_025238Add
BLAST
Alternative sequencei97 – 271175Missing in isoform 4. 1 PublicationVSP_025240Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BD418666 mRNA. No translation available.
AL157782, AC073587, AC023282 Genomic DNA. Translation: CAH70332.1. Different termination.
AL157782, AC023282, AC073587 Genomic DNA. Translation: CAH70333.1. Sequence problems.
BC101267 mRNA. Translation: AAI01268.1.
BC101268 mRNA. Translation: AAI01269.1.
BC101269 mRNA. Translation: AAI01270.1.
BC122535 mRNA. Translation: AAI22536.1.
BC132787 mRNA. Translation: AAI32788.1.
CCDSiCCDS41573.1. [Q5VZY2-1]
RefSeqiNP_001025230.1. NM_001030059.1. [Q5VZY2-1]
UniGeneiHs.40479.

Genome annotation databases

EnsembliENST00000398250; ENSP00000381302; ENSG00000203805. [Q5VZY2-1]
GeneIDi196051.
KEGGihsa:196051.
UCSCiuc001lev.1. human. [Q5VZY2-1]
uc001lew.1. human. [Q5VZY2-4]
uc009xzl.1. human. [Q5VZY2-2]

Polymorphism and mutation databases

BioMutaiPPAPDC1A.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BD418666 mRNA. No translation available.
AL157782, AC073587, AC023282 Genomic DNA. Translation: CAH70332.1. Different termination.
AL157782, AC023282, AC073587 Genomic DNA. Translation: CAH70333.1. Sequence problems.
BC101267 mRNA. Translation: AAI01268.1.
BC101268 mRNA. Translation: AAI01269.1.
BC101269 mRNA. Translation: AAI01270.1.
BC122535 mRNA. Translation: AAI22536.1.
BC132787 mRNA. Translation: AAI32788.1.
CCDSiCCDS41573.1. [Q5VZY2-1]
RefSeqiNP_001025230.1. NM_001030059.1. [Q5VZY2-1]
UniGeneiHs.40479.

3D structure databases

ProteinModelPortaliQ5VZY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128189. 1 interaction.
STRINGi9606.ENSP00000381302.

PTM databases

DEPODiQ5VZY2.
PhosphoSiteiQ5VZY2.

Polymorphism and mutation databases

BioMutaiPPAPDC1A.
DMDMi147721098.

Proteomic databases

PaxDbiQ5VZY2.
PRIDEiQ5VZY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398250; ENSP00000381302; ENSG00000203805. [Q5VZY2-1]
GeneIDi196051.
KEGGihsa:196051.
UCSCiuc001lev.1. human. [Q5VZY2-1]
uc001lew.1. human. [Q5VZY2-4]
uc009xzl.1. human. [Q5VZY2-2]

Organism-specific databases

CTDi196051.
GeneCardsiGC10P122207.
HGNCiHGNC:23531. PPAPDC1A.
HPAiHPA045188.
neXtProtiNX_Q5VZY2.
PharmGKBiPA134907381.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0671.
GeneTreeiENSGT00510000046723.
HOGENOMiHOG000215098.
HOVERGENiHBG056764.
InParanoidiQ5VZY2.
OMAiFAVICVY.
OrthoDBiEOG79CZ0D.
PhylomeDBiQ5VZY2.
TreeFamiTF323722.

Enzyme and pathway databases

BRENDAi3.1.3.4. 2681.
3.1.3.81. 2681.
ReactomeiREACT_160158. Role of phospholipids in phagocytosis.

Miscellaneous databases

ChiTaRSiPPAPDC1A. human.
GeneWikiiPPAPDC1A.
GenomeRNAii196051.
NextBioi89410.
PROiQ5VZY2.

Gene expression databases

BgeeiQ5VZY2.
CleanExiHS_PPAPDC1A.
ExpressionAtlasiQ5VZY2. baseline and differential.
GenevestigatoriQ5VZY2.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
IPR028668. PPAPDC1A/PPAPDC1B.
[Graphical view]
PANTHERiPTHR10165:SF90. PTHR10165:SF90. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase."
    Takeuchi M., Harigai M., Momohara S., Ball E., Abe J., Furuichi K., Kamatani N.
    Gene 399:174-180(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, ENZYME REGULATION, CATALYTIC ACTIVITY, REGION, MUTAGENESIS OF ARG-109; HIS-146 AND HIS-202, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Tissue: Brain.

Entry informationi

Entry nameiPPC1A_HUMAN
AccessioniPrimary (citable) accession number: Q5VZY2
Secondary accession number(s): A2RU82
, Q08EQ2, Q0IIP2, Q495B4, Q5VZY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: April 29, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.