Q5VZE5 (NAA35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-alpha-acetyltransferase 35, NatC auxiliary subunit

Alternative name(s):
Embryonic growth-associated protein homolog
Protein MAK10 homolog

Gene names

Name:	NAA35
Synonyms:	EGAP, MAK10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates proliferation of smooth muscle cells By similarity. Component of the N-terminal acetyltransferase C (NatC) complex which may catalyze acetylation of N-terminal methionine residues. Ref.7
Subunit structure	Interacts with NAA50 By similarity. Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, LSMD1 and NAA30.
Subcellular location	Cytoplasm Ref.7.
Sequence similarities	Belongs to the MAK10 family.
Sequence caution	The sequence BAB15097.1 differs from that shown. Reason: Frameshift at position 518. The sequence BAB15435.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	N-alpha-acetyltransferase 35, NatC auxiliary subunit		PRO_0000308614
Experimental info
	Sequence conflict	229	1	Q → R in BAB15097. Ref.1
	Sequence conflict	487	1	C → W in BAB15097. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5VZE5 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: A5612BA0CE1BAE3F	FASTA	725	83,639
	10 20 30 40 50 60 MVMKASVDDD DSGWELSMPE KMEKSNTNWV DITQDFEEAC RELKLGELLH DKLFGLFEAM 70 80 90 100 110 120 SAIEMMDPKM DAGMIGNQVN RKVLNFEQAI KDGTIKIKDL TLPELIGIMD TCFCCLITWL 130 140 150 160 170 180 EGHSLAQTVF TCLYIHNPDF IEDPAMKAFA LGILKICDIA REKVNKAAVF EEEDFQSMTY 190 200 210 220 230 240 GFKMANSVTD LRVTGMLKDV EDDMQRRVKS TRSRQGEERD PEVELEHQQC LAVFSRVKFT 250 260 270 280 290 300 RVLLTVLIAF TKKETSAVAE AQKLMVQAAD LLSAIHNSLH HGIQAQNDTT KGDHPIMMGF 310 320 330 340 350 360 EPLVNQRLLP PTFPRYAKII KREEMVNYFA RLIDRIKTVC EVVNLTNLHC ILDFFCEFSE 370 380 390 400 410 420 QSPCVLSRSL LQTTFLVDNK KVFGTHLMQD MVKDALRSFV SPPVLSPKCY LYNNHQAKDC 430 440 450 460 470 480 IDSFVTHCVR PFCSLIQIHG HNRARQRDKL GHILEEFATL QDEAEKVDAA LHTMLLKQEP 490 500 510 520 530 540 QRQHLACLGT WVLYHNLRIM IQYLLSGFEL ELYSMHEYYY IYWYLSEFLY AWLMSTLSRA 550 560 570 580 590 600 DGSQMAEERI MEEQQKGRSS KKTKKKKKVR PLSREITMSQ AYQNMCAGMF KTMVAFDMDG 610 620 630 640 650 660 KVRKPKFELD SEQVRYEHRF APFNSVMTPP PVHYLQFKEM SDLNKYSPPP QSPELYVAAS 670 680 690 700 710 720 KHFQQAKMIL ENIPNPDHEV NRILKVAKPN FVVMKLLAGG HKKESKVPPE FDFSAHKYFP VVKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Small intestine.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development." Wenzlau J.M., Garl P.J., Simpson P., Stenmark K.R., West J., Artinger K.B., Nemenoff R.A., Weiser-Evans M.C.M. Circ. Res. 98:846-855(2006) [PubMed: 16484612] [Abstract] Cited for: IDENTIFICATION.
[6]	"A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates." Polevoda B., Arnesen T., Sherman F. BMC Proc. 3:S2-S2(2009) [PubMed: 19660095] [Abstract] Cited for: NOMENCLATURE.
[7]	"Knockdown of human N alpha-terminal acetyltransferase complex C leads to p53-dependent apoptosis and aberrant human Arl8b localization." Starheim K.K., Gromyko D., Evjenth R., Ryningen A., Varhaug J.E., Lillehaug J.R., Arnesen T. Mol. Cell. Biol. 29:3569-3581(2009) [PubMed: 19398576] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN NATC COMPLEX, SUBCELLULAR LOCATION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK025266 mRNA. Translation: BAB15097.1. Frameshift. AK026296 mRNA. Translation: BAB15435.1. Different initiation. AL161447, AL161453, AL353743 Genomic DNA. Translation: CAH73155.1. AL353743, AL161447, AL161453 Genomic DNA. Translation: CAI12659.1. AL161453, AL161447, AL353743 Genomic DNA. Translation: CAI16313.1. CH471089 Genomic DNA. Translation: EAW62702.1. BC117427 mRNA. Translation: AAI17428.1. BC117429 mRNA. Translation: AAI17430.1.
IPI	IPI00396185.
RefSeq	NP_078911.3. NM_024635.3.
UniGene	Hs.436098.
3D structure databases
ProteinModelPortal	Q5VZE5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5VZE5.
Polymorphism databases
DMDM	74747795.
Proteomic databases
PRIDE	Q5VZE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361671; ENSP00000354972; ENSG00000135040.
GeneID	60560.
KEGG	hsa:60560.
UCSC	uc004aoi.2. human.
Organism-specific databases
CTD	60560.
GeneCards	GC09P088556.
H-InvDB	HIX0008139.
HGNC	HGNC:24340. NAA35.
HPA	HPA021547.
neXtProt	NX_Q5VZE5.
PharmGKB	PA134865889. PA165585932.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000002445.
HOGENOM	HBG447204.
HOVERGEN	HBG099871.
InParanoid	Q5VZE5.
OMA	CAGMYKT.
OrthoDB	EOG4FBHSC.
PhylomeDB	Q5VZE5.
Gene expression databases
ArrayExpress	Q5VZE5.
Bgee	Q5VZE5.
CleanEx	HS_MAK10.
Genevestigator	Q5VZE5.
Family and domain databases
InterPro	IPR007244. NatC_a_AcTrfase. [Graphical view]
Pfam	PF04112. Mak10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	65445.

Entry information

Entry name

NAA35_HUMAN

Accession

Primary (citable) accession number: Q5VZE5
Secondary accession number(s): Q9H631, Q9H703

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.