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Protein

DENN domain-containing protein 4C

Gene

DENND4C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 4C
Gene namesi
Name:DENND4C
Synonyms:C9orf55, C9orf55B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:26079. DENND4C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134939495.

Polymorphism and mutation databases

DMDMi158937337.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896971 – 1673DENN domain-containing protein 4CAdd BLAST1673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei467PhosphoserineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei505PhosphoserineCombined sources1
Modified residuei729PhosphoserineCombined sources1
Modified residuei732PhosphoserineCombined sources1
Modified residuei737PhosphoserineCombined sources1
Modified residuei739PhosphothreonineCombined sources1
Modified residuei753PhosphoserineCombined sources1
Modified residuei760PhosphoserineCombined sources1
Modified residuei767PhosphoserineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei825PhosphoserineCombined sources1
Modified residuei863PhosphoserineCombined sources1
Modified residuei890PhosphoserineCombined sources1
Modified residuei948PhosphoserineCombined sources1
Modified residuei989PhosphoserineCombined sources1
Modified residuei1008PhosphoserineCombined sources1
Modified residuei1016PhosphoserineBy similarity1
Modified residuei1042PhosphoserineCombined sources1
Modified residuei1089PhosphoserineCombined sources1
Modified residuei1101PhosphoserineCombined sources1
Modified residuei1110PhosphoserineCombined sources1
Modified residuei1387PhosphoserineCombined sources1
Modified residuei1391PhosphoserineCombined sources1
Modified residuei1393PhosphoserineCombined sources1
Modified residuei1404PhosphoserineBy similarity1
Modified residuei1563PhosphoserineCombined sources1
Isoform 2 (identifier: Q5VZ89-2)
Modified residuei144PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in response to insulin.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5VZ89.
MaxQBiQ5VZ89.
PaxDbiQ5VZ89.
PeptideAtlasiQ5VZ89.
PRIDEiQ5VZ89.

PTM databases

iPTMnetiQ5VZ89.
PhosphoSitePlusiQ5VZ89.

Expressioni

Gene expression databases

BgeeiENSG00000137145.
CleanExiHS_DENND4C.

Organism-specific databases

HPAiHPA014917.
HPA015096.

Interactioni

Protein-protein interaction databases

BioGridi120799. 10 interactors.
IntActiQ5VZ89. 9 interactors.
STRINGi9606.ENSP00000369802.

Structurei

3D structure databases

ProteinModelPortaliQ5VZ89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 40UDENNAdd BLAST40
Domaini72 – 256DENNPROSITE-ProRule annotationAdd BLAST185
Domaini322 – 396dDENNPROSITE-ProRule annotationAdd BLAST75
Repeati585 – 619PPRAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1199 – 1203Poly-Ser5

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
HOVERGENiHBG058313.
InParanoidiQ5VZ89.
KOiK20163.
PhylomeDBiQ5VZ89.
TreeFamiTF313237.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VZ89-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGATIECWDP ETKYPLPVFS TFVLTGSSAK KVYGAAIQFY EPYSRELLSE
60 70 80 90 100
KQLMHLGLLT PVERKMVSKS INTNKCICLL SHWPFFEAFR KFLMFIYKLS
110 120 130 140 150
VSGPHPLPIE KHISHFMQNI PFPSPQRPRI LVQLSVHDAL ILSQPVSTPL
160 170 180 190 200
PLSGANFSTL LMNLGPENCA TLLLFVLLES KILLHSLRPA VLTGVAEAVV
210 220 230 240 250
AMIFPFQWQC PYIPLCPLSL AAVLSAPLPF IVGVDSRYFD LHDPPQDVVC
260 270 280 290 300
IDLDTNMLYV SDEKKNMNWK QLPKKPCKNL LSTLKKLYPQ LSSVHQKTQE
310 320 330 340 350
GSAIDMTPIE ADFSWQKKMT QLEMEIQEAF LRFMASILKG YRTYLRPITE
360 370 380 390 400
APSNKATAAD SLFDRQGFLK SRDRAYAKFY TLLSKTQIFI RFIEECSFVS
410 420 430 440 450
DKDTGLAFFD DCIEKLFPDK GTEKTDKVDF DSAEDTRLIE LDDSQKSEHT
460 470 480 490 500
VFIMPPEPPP DDGKDLSPKY SYKYFPRLDL KLFDRPQELK LCFSRHPTGN
510 520 530 540 550
SITKSPPLMA KRTKQEIKTA HKLAKRCYTN PPQWAKCLFS HCYSLWFICL
560 570 580 590 600
PAYVRVSHPK VRALQQAYDV LIKMRKTDVD PLDEVCYRVV MQLCGLWGHP
610 620 630 640 650
VLAVRVLFEM KTARIKPNAI TYGYYNKVVL ESPWPSSTRS GIFLWTKVRN
660 670 680 690 700
VVRGLAQFRQ PLKKTVQRSQ VSSISGGQSD QGYGSKDELI KDDAEIHVPE
710 720 730 740 750
EQAARELITK TKMQTEEVCD ASAIVAKHSQ PSPEPHSPTE PPAWGSSIVK
760 770 780 790 800
VPSGIFDVNS RKSSTGSISN VLFSTQDPVE DAVFGEATNL KKNGDRGEKR
810 820 830 840 850
QKHFPERSCS FSSESRAGML LKKSSLDSNS SEMAIMMGAD AKILTAALTC
860 870 880 890 900
PKTSLLHIAR THSFENVSCH LPDSRTCMSE STWNPEHRSS PVPEMLEESQ
910 920 930 940 950
ELLEPVVDDV PKTTATVDTY ESLLSDSNSN QSRDLKTVSK DLRNKRSSLY
960 970 980 990 1000
GIAKVVQRED VETGLDPLSL LATECTGGKT PDSEDKLFSP VIARNLADEI
1010 1020 1030 1040 1050
ESYMNLKSPL GSKSSSMELH REENRESGMT TAFIHALERR SSLPLDHGSP
1060 1070 1080 1090 1100
AQENPESEKS SPAVSRSKTF TGRFKQQTPS RTHKERSTSL SALVRSSPHG
1110 1120 1130 1140 1150
SLGSVVNSLS GLKLDNILSG PKIDVLKSGM KQAATVASKM WVAVASAYSY
1160 1170 1180 1190 1200
SDDEEETNRD YSFPAGLEDH ILGENISPNT SISGLVPSEL TQSNTSLGSS
1210 1220 1230 1240 1250
SSSGDVGKLH YPTGEVPFPR GMKGQDFEKS DHGSSQNTSM SSIYQNCAME
1260 1270 1280 1290 1300
VLMSSCSQCR ACGALVYDEE IMAGWTADDS NLNTACPFCK SNFLPLLNIE
1310 1320 1330 1340 1350
FKDLRGSASF FLKPSTSGDS LQSGSIPLAN ESLEHKPVSS LAEPDLINFM
1360 1370 1380 1390 1400
DFPKHNQIIT EETGSAVEPS DEIKRASGDV QTMKISSVPN SLSKRNVSLT
1410 1420 1430 1440 1450
RSHSVGGPLQ NIDFTQRPFH GISTVSLPNS LQEVVDPLGK RPNPPPVSVP
1460 1470 1480 1490 1500
YLSPLVLRKE LESLLENEGD QVIHTSSFIN QHPIIFWNLV WYFRRLDLPS
1510 1520 1530 1540 1550
NLPGLILTSE HCNEGVQLPL SSLSQDSKLV YIQLLWDNIN LHQEPREPLY
1560 1570 1580 1590 1600
VSWRNFNSEK KSSLLSEEQQ ETSTLVETIR QSIQHNNVLK PINLLSQQMK
1610 1620 1630 1640 1650
PGMKRQRSLY REILFLSLVS LGRENIDIEA FDNEYGIAYN SLSSEILERL
1660 1670
QKIDAPPSAS VEWCRKCFGA PLI
Length:1,673
Mass (Da):186,857
Last modified:August 21, 2007 - v2
Checksum:iEC6A7115E22A7B02
GO
Isoform 2 (identifier: Q5VZ89-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-573: Missing.
     675-675: S → SALQNVTGGSDGDTVSHGSVDSSNDANNGEHTVFVRDLIRLESIDNHSST
     764-766: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:1,146
Mass (Da):126,244
Checksum:i6E1B04E6EB21C576
GO
Isoform 3 (identifier: Q5VZ89-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-573: Missing.
     574-818: Missing.
     1608-1609: SL → GI
     1610-1673: Missing.

Show »
Length:791
Mass (Da):86,970
Checksum:i76BF2D89AD747D32
GO
Isoform 4 (identifier: Q5VZ89-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-573: Missing.
     574-818: Missing.
     819-1003: Missing.

Note: No experimental confirmation available.
Show »
Length:670
Mass (Da):73,931
Checksum:iAA59FEFF544AC1A3
GO
Isoform 5 (identifier: Q5VZ89-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1370-1387: SDEIKRASGDVQTMKISS → RYQRVQRPLYVVINGVPL
     1388-1609: Missing.
     1610-1673: Missing.

Show »
Length:1,387
Mass (Da):154,479
Checksum:iDFA5CF2CC37C4D7C
GO
Isoform 6 (identifier: Q5VZ89-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1608-1673: Missing.

Note: No experimental confirmation available.
Show »
Length:1,607
Mass (Da):179,409
Checksum:iB03ED897D7F51D85
GO

Sequence cautioni

The sequence BAA91294 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA91478 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92039 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15221 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAH10466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAM21141 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447S → G in BAC86313 (PubMed:14702039).Curated1
Sequence conflicti668 – 675RSQVSSIS → CKSAFFLT in CAH10466 (PubMed:17974005).Curated8
Sequence conflicti1061S → P in BAA91294 (PubMed:14702039).Curated1
Sequence conflicti1420H → R in BAA91478 (PubMed:14702039).Curated1
Sequence conflicti1533Q → H in BAA92039 (PubMed:14702039).Curated1
Sequence conflicti1626I → V in CAH10466 (PubMed:17974005).Curated1
Sequence conflicti1632D → G in CAH10466 (PubMed:17974005).Curated1
Sequence conflicti1657P → A in CAD39177 (PubMed:17974005).Curated1
Sequence conflicti1661V → A in CAH10466 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061640991V → G.Corresponds to variant rs34267952dbSNPEnsembl.1
Natural variantiVAR_0228911030T → A.Corresponds to variant rs17818730dbSNPEnsembl.1
Natural variantiVAR_0228921107N → H.Corresponds to variant rs6475322dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0273771 – 573Missing in isoform 2, isoform 3 and isoform 4. 1 PublicationAdd BLAST573
Alternative sequenceiVSP_027378574 – 818Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST245
Alternative sequenceiVSP_027379675S → SALQNVTGGSDGDTVSHGSV DSSNDANNGEHTVFVRDLIR LESIDNHSST in isoform 2. Curated1
Alternative sequenceiVSP_027380764 – 766Missing in isoform 2. Curated3
Alternative sequenceiVSP_027381819 – 1003Missing in isoform 4. CuratedAdd BLAST185
Alternative sequenceiVSP_0273821370 – 1387SDEIK…MKISS → RYQRVQRPLYVVINGVPL in isoform 5. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_0273831388 – 1609Missing in isoform 5. 1 PublicationAdd BLAST222
Alternative sequenceiVSP_0273851608 – 1673Missing in isoform 6. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_0273841608 – 1609SL → GI in isoform 3. 1 Publication2
Alternative sequenceiVSP_0273861610 – 1673Missing in isoform 3 and isoform 5. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161909, AL391834 Genomic DNA. Translation: CAH70581.2.
AL391834 Genomic DNA. Translation: CAM21138.1.
AL391834 Genomic DNA. Translation: CAM21139.1.
AL391834 Genomic DNA. Translation: CAM21140.1.
AL391834 Genomic DNA. Translation: CAM21141.1. Different initiation.
AL391834, AL161909 Genomic DNA. Translation: CAM21148.1.
AK000627 mRNA. Translation: BAA91294.1. Different initiation.
AK001046 mRNA. Translation: BAA91478.1. Different initiation.
AK002020 mRNA. Translation: BAA92039.1. Different initiation.
AK025705 mRNA. Translation: BAB15221.1. Different initiation.
AK125842 mRNA. Translation: BAC86313.1.
AL834521 mRNA. Translation: CAD39177.1.
CR627367 mRNA. Translation: CAH10466.1. Different initiation.
RefSeqiNP_060395.5. NM_017925.6.
UniGeneiHs.249591.

Genome annotation databases

EnsembliENST00000380432; ENSP00000369797; ENSG00000137145.
GeneIDi55667.
KEGGihsa:55667.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161909, AL391834 Genomic DNA. Translation: CAH70581.2.
AL391834 Genomic DNA. Translation: CAM21138.1.
AL391834 Genomic DNA. Translation: CAM21139.1.
AL391834 Genomic DNA. Translation: CAM21140.1.
AL391834 Genomic DNA. Translation: CAM21141.1. Different initiation.
AL391834, AL161909 Genomic DNA. Translation: CAM21148.1.
AK000627 mRNA. Translation: BAA91294.1. Different initiation.
AK001046 mRNA. Translation: BAA91478.1. Different initiation.
AK002020 mRNA. Translation: BAA92039.1. Different initiation.
AK025705 mRNA. Translation: BAB15221.1. Different initiation.
AK125842 mRNA. Translation: BAC86313.1.
AL834521 mRNA. Translation: CAD39177.1.
CR627367 mRNA. Translation: CAH10466.1. Different initiation.
RefSeqiNP_060395.5. NM_017925.6.
UniGeneiHs.249591.

3D structure databases

ProteinModelPortaliQ5VZ89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120799. 10 interactors.
IntActiQ5VZ89. 9 interactors.
STRINGi9606.ENSP00000369802.

PTM databases

iPTMnetiQ5VZ89.
PhosphoSitePlusiQ5VZ89.

Polymorphism and mutation databases

DMDMi158937337.

Proteomic databases

EPDiQ5VZ89.
MaxQBiQ5VZ89.
PaxDbiQ5VZ89.
PeptideAtlasiQ5VZ89.
PRIDEiQ5VZ89.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380432; ENSP00000369797; ENSG00000137145.
GeneIDi55667.
KEGGihsa:55667.

Organism-specific databases

CTDi55667.
GeneCardsiDENND4C.
H-InvDBHIX0007946.
HGNCiHGNC:26079. DENND4C.
HPAiHPA014917.
HPA015096.
neXtProtiNX_Q5VZ89.
PharmGKBiPA134939495.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
HOVERGENiHBG058313.
InParanoidiQ5VZ89.
KOiK20163.
PhylomeDBiQ5VZ89.
TreeFamiTF313237.

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiDENND4C. human.
GenomeRNAii55667.
PROiQ5VZ89.

Gene expression databases

BgeeiENSG00000137145.
CleanExiHS_DENND4C.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEN4C_HUMAN
AccessioniPrimary (citable) accession number: Q5VZ89
Secondary accession number(s): A2A3R1
, A2A3R2, A2A3R3, A2A3R9, Q6AI48, Q6ZUB3, Q8NCY7, Q9H6N4, Q9NUT1, Q9NWA5, Q9NWT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: August 21, 2007
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.