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Protein

Terminal uridylyltransferase 7

Gene

ZCCHC6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.3 Publications

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1058Magnesium or manganese; catalyticBy similarity1
Metal bindingi1060Magnesium or manganese; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri963 – 980CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1345 – 1362CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1451 – 1468CCHC-type 3PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • RNA uridylyltransferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome
  • RNA 3'-end processing Source: UniProtKB

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandMagnesium, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.7.52. 2681.
ReactomeiR-HSA-429947. Deadenylation of mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Terminal uridylyltransferase 7 (EC:2.7.7.52)
Short name:
TUTase 7
Alternative name(s):
Zinc finger CCHC domain-containing protein 6
Gene namesi
Name:ZCCHC6
Synonyms:HS2, KIAA1711, TUT7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000083223.17.
HGNCiHGNC:25817. ZCCHC6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000083223.
PharmGKBiPA134971144.

Polymorphism and mutation databases

BioMutaiZCCHC6.
DMDMi67462100.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001509571 – 1495Terminal uridylyltransferase 7Add BLAST1495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphothreonineCombined sources1
Modified residuei64PhosphothreonineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei172PhosphoserineCombined sources1
Modified residuei600PhosphoserineCombined sources1
Modified residuei844PhosphoserineCombined sources1
Modified residuei893PhosphoserineBy similarity1
Modified residuei939PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5VYS8.
MaxQBiQ5VYS8.
PaxDbiQ5VYS8.
PeptideAtlasiQ5VYS8.
PRIDEiQ5VYS8.

PTM databases

iPTMnetiQ5VYS8.
PhosphoSitePlusiQ5VYS8.

Expressioni

Gene expression databases

BgeeiENSG00000083223.
CleanExiHS_ZCCHC6.
ExpressionAtlasiQ5VYS8. baseline and differential.
GenevisibleiQ5VYS8. HS.

Organism-specific databases

HPAiHPA020615.
HPA020620.

Interactioni

Protein-protein interaction databases

BioGridi122795. 31 interactors.
IntActiQ5VYS8. 18 interactors.
MINTiMINT-4718069.
STRINGi9606.ENSP00000365130.

Structurei

Secondary structure

11495
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi995 – 1012Combined sources18
Helixi1016 – 1034Combined sources19
Beta strandi1040 – 1045Combined sources6
Helixi1046 – 1048Combined sources3
Beta strandi1059 – 1065Combined sources7
Helixi1071 – 1073Combined sources3
Helixi1076 – 1087Combined sources12
Beta strandi1093 – 1099Combined sources7
Beta strandi1101 – 1104Combined sources4
Beta strandi1106 – 1111Combined sources6
Turni1112 – 1115Combined sources4
Beta strandi1116 – 1123Combined sources8
Helixi1125 – 1138Combined sources14
Helixi1142 – 1158Combined sources17
Helixi1163 – 1165Combined sources3
Helixi1170 – 1183Combined sources14
Beta strandi1184 – 1186Combined sources3
Helixi1192 – 1194Combined sources3
Beta strandi1197 – 1200Combined sources4
Helixi1217 – 1219Combined sources3
Helixi1220 – 1223Combined sources4
Turni1225 – 1228Combined sources4
Helixi1234 – 1247Combined sources14
Turni1251 – 1253Combined sources3
Beta strandi1254 – 1256Combined sources3
Helixi1266 – 1269Combined sources4
Beta strandi1273 – 1275Combined sources3
Turni1288 – 1291Combined sources4
Helixi1294 – 1312Combined sources19
Turni1316 – 1321Combined sources6
Beta strandi1322 – 1324Combined sources3
Helixi1325 – 1329Combined sources5
Helixi1332 – 1335Combined sources4
Beta strandi1344 – 1346Combined sources3
Turni1348 – 1350Combined sources3
Beta strandi1353 – 1355Combined sources3
Helixi1357 – 1359Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W0BX-ray2.61A/B/C983-1365[»]
5W0MX-ray2.30A/B/C983-1365[»]
5W0NX-ray2.50A/B/C963-1365[»]
5W0OX-ray2.49A/B983-1365[»]
ProteinModelPortaliQ5VYS8.
SMRiQ5VYS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 600PAP-associated 1Add BLAST50
Domaini1233 – 1286PAP-associated 2Add BLAST54

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 23Poly-Asp4
Compositional biasi815 – 936Glu-richAdd BLAST122
Compositional biasi1367 – 1370Poly-Arg4

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri963 – 980CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1345 – 1362CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1451 – 1468CCHC-type 3PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
InParanoidiQ5VYS8.
KOiK13291.
OMAiKYFALPH.
OrthoDBiEOG091G01G7.
PhylomeDBiQ5VYS8.
TreeFamiTF315661.

Family and domain databases

Gene3Di4.10.60.10. 4 hits.
InterProiView protein in InterPro
IPR003604. Matrin/U1-like-C_Znf_C2H2.
IPR002058. PAP_assoc.
IPR002934. Polymerase_NTP_transf_dom.
IPR001878. Znf_CCHC.
IPR036875. Znf_CCHC_sf.
PfamiView protein in Pfam
PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 2 hits.
PF00098. zf-CCHC. 3 hits.
SMARTiView protein in SMART
SM00343. ZnF_C2HC. 3 hits.
SM00451. ZnF_U1. 1 hit.
SUPFAMiSSF57756. SSF57756. 3 hits.
PROSITEiView protein in PROSITE
PS50158. ZF_CCHC. 3 hits.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VYS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDTAKPYFV KRTKDRGTMD DDDFRRGHPQ QDYLIIDDHA KGHGSKMEKG
60 70 80 90 100
LQKKKITPGN YGNTPRKGPC AVSSNPYAFK NPIYSQPAWM NDSHKDQSKR
110 120 130 140 150
WLSDEHTGNS DNWREFKPGP RIPVINRQRK DSFQENEDGY RWQDTRGCRT
160 170 180 190 200
VRRLFHKDLT SLETTSEMEA GSPENKKQRS RPRKPRKTRN EENEQDGDLE
210 220 230 240 250
GPVIDESVLS TKELLGLQQA EERLKRDCID RLKRRPRNYP TAKYTCRLCD
260 270 280 290 300
VLIESIAFAH KHIKEKRHKK NIKEKQEEEL LTTLPPPTPS QINAVGIAID
310 320 330 340 350
KVVQEFGLHN ENLEQRLEIK RIMENVFQHK LPDCSLRLYG SSCSRLGFKN
360 370 380 390 400
SDVNIDIQFP AIMSQPDVLL LVQECLKNSD SFIDVDADFH ARVPVVVCRE
410 420 430 440 450
KQSGLLCKVS AGNENACLTT KHLTALGKLE PKLVPLVIAF RYWAKLCSID
460 470 480 490 500
RPEEGGLPPY VFALMAIFFL QQRKEPLLPV YLGSWIEGFS LSKLGNFNLQ
510 520 530 540 550
DIEKDVVIWE HTDSAAGDTG ITKEEAPRET PIKRGQVSLI LDVKHQPSVP
560 570 580 590 600
VGQLWVELLR FYALEFNLAD LVISIRVKEL VSRELKDWPK KRIAIEDPYS
610 620 630 640 650
VKRNVARTLN SQPVFEYILH CLRTTYKYFA LPHKITKSSL LKPLNAITCI
660 670 680 690 700
SEHSKEVINH HPDVQTKDDK LKNSVLAQGP GATSSAANTC KVQPLTLKET
710 720 730 740 750
AESFGSPPKE EMGNEHISVH PENSDCIQAD VNSDDYKGDK VYHPETGRKN
760 770 780 790 800
EKEKVGRKGK HLLTVDQKRG EHVVCGSTRN NESESTLDLE GFQNPTAKEC
810 820 830 840 850
EGLATLDNKA DLDGESTEGT EELEDSLNHF THSVQGQTSE MIPSDEEEED
860 870 880 890 900
DEEEEEEEEP RLTINQREDE DGMANEDELD NTYTGSGDED ALSEEDDELG
910 920 930 940 950
EAAKYEDVKE CGKHVERALL VELNKISLKE ENVCEEKNSP VDQSDFFYEF
960 970 980 990 1000
SKLIFTKGKS PTVVCSLCKR EGHLKKDCPE DFKRIQLEPL PPLTPKFLNI
1010 1020 1030 1040 1050
LDQVCIQCYK DFSPTIIEDQ AREHIRQNLE SFIRQDFPGT KLSLFGSSKN
1060 1070 1080 1090 1100
GFGFKQSDLD VCMTINGLET AEGLDCVRTI EELARVLRKH SGLRNILPIT
1110 1120 1130 1140 1150
TAKVPIVKFF HLRSGLEVDI SLYNTLALHN TRLLSAYSAI DPRVKYLCYT
1160 1170 1180 1190 1200
MKVFTKMCDI GDASRGSLSS YAYTLMVLYF LQQRNPPVIP VLQEIYKGEK
1210 1220 1230 1240 1250
KPEIFVDGWN IYFFDQIDEL PTYWSECGKN TESVGQLWLG LLRFYTEEFD
1260 1270 1280 1290 1300
FKEHVISIRR KSLLTTFKKQ WTSKYIVIED PFDLNHNLGA GLSRKMTNFI
1310 1320 1330 1340 1350
MKAFINGRRV FGIPVKGFPK DYPSKMEYFF DPDVLTEGEL APNDRCCRIC
1360 1370 1380 1390 1400
GKIGHFMKDC PMRRKVRRRR DQEDALNQRY PENKEKRSKE DKEIHNKYTE
1410 1420 1430 1440 1450
REVSTKEDKP IQCTPQKAKP MRAAADLGRE KILRPPVEKW KRQDDKDLRE
1460 1470 1480 1490
KRCFICGREG HIKKECPQFK GSSGSLSSKY MTQGKASAKR TQQES
Length:1,495
Mass (Da):171,229
Last modified:December 7, 2004 - v1
Checksum:i1AAB0F5B37266FF9
GO
Isoform 2 (identifier: Q5VYS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     597-615: DPYSVKRNVARTLNSQPVF → GISKCLSYSPPLFFLKVPV
     616-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:615
Mass (Da):70,475
Checksum:iE3B7FEE22E438BBD
GO
Isoform 3 (identifier: Q5VYS8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     538-544: SLILDVK → VSSLLCR
     545-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):62,261
Checksum:iDE285E0532C43340
GO
Isoform 4 (identifier: Q5VYS8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     363-486: MSQPDVLLLV...LLPVYLGSWI → I
     960-1072: Missing.

Note: No experimental confirmation available.
Show »
Length:1,259
Mass (Da):144,656
Checksum:i2DFDA89240222042
GO
Isoform 5 (identifier: Q5VYS8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     403-412: SGLLCKVSAG → RSHFFKLFIY
     413-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:412
Mass (Da):47,903
Checksum:i73F48669DDBD99CB
GO
Isoform 6 (identifier: Q5VYS8-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1157-1194: Missing.

Note: No experimental confirmation available.
Show »
Length:1,457
Mass (Da):166,970
Checksum:iF5F376C95EF2CEE5
GO

Sequence cautioni

The sequence BAB14584 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB71052 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157K → R in BAB70951 (PubMed:14702039).Curated1
Sequence conflicti316R → T in CAE46038 (PubMed:17974005).Curated1
Sequence conflicti514S → N in CAE46038 (PubMed:17974005).Curated1
Sequence conflicti900G → V in CAI45944 (PubMed:17974005).Curated1
Sequence conflicti937Missing in CAI45944 (PubMed:17974005).Curated1
Sequence conflicti937Missing in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1104V → M in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1319P → S in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1474G → D in CAI45944 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05375340A → V. Corresponds to variant dbSNP:rs2378695Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013832363 – 486MSQPD…LGSWI → I in isoform 4. 1 PublicationAdd BLAST124
Alternative sequenceiVSP_013833403 – 412SGLLCKVSAG → RSHFFKLFIY in isoform 5. 1 Publication10
Alternative sequenceiVSP_013834413 – 1495Missing in isoform 5. 1 PublicationAdd BLAST1083
Alternative sequenceiVSP_013835538 – 544SLILDVK → VSSLLCR in isoform 3. 1 Publication7
Alternative sequenceiVSP_013836545 – 1495Missing in isoform 3. 1 PublicationAdd BLAST951
Alternative sequenceiVSP_013837597 – 615DPYSV…SQPVF → GISKCLSYSPPLFFLKVPV in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_013838616 – 1495Missing in isoform 2. 1 PublicationAdd BLAST880
Alternative sequenceiVSP_013839960 – 1072Missing in isoform 4. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_0138401157 – 1194Missing in isoform 6. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL832026 mRNA. Translation: CAH56219.1.
AL832193 mRNA. Translation: CAH56214.1.
BX641077 mRNA. Translation: CAE46038.1.
CR933643 mRNA. Translation: CAI45944.1.
CR933644 mRNA. Translation: CAI45945.1.
CR936608 mRNA. Translation: CAI56753.1.
AL353678, AL137849 Genomic DNA. Translation: CAH71514.1. Sequence problems.
AL353678, AL137849 Genomic DNA. Translation: CAH71515.1. Sequence problems.
AL353678, AL137849 Genomic DNA. Translation: CAH71518.1.
AL353678, AL137849 Genomic DNA. Translation: CAH71519.1.
AL137849, AL353678 Genomic DNA. Translation: CAI14918.1. Sequence problems.
AL137849, AL353678 Genomic DNA. Translation: CAI14919.1.
AL137849, AL353678 Genomic DNA. Translation: CAI14920.1.
AL137849, AL353678 Genomic DNA. Translation: CAI14921.1.
BC032456 mRNA. Translation: AAH32456.1.
AK023471 mRNA. Translation: BAB14584.1. Different initiation.
AK055546 mRNA. Translation: BAB70951.1.
AK055948 mRNA. Translation: BAB71052.1. Different initiation.
AB051498 mRNA. Translation: BAB21802.1.
CCDSiCCDS35057.1. [Q5VYS8-1]
CCDS55323.1. [Q5VYS8-4]
RefSeqiNP_001171988.1. NM_001185059.1. [Q5VYS8-1]
NP_001172003.1. NM_001185074.1. [Q5VYS8-4]
NP_001317647.1. NM_001330718.1.
NP_078893.2. NM_024617.3. [Q5VYS8-1]
XP_016870619.1. XM_017015130.1. [Q5VYS8-1]
XP_016870620.1. XM_017015131.1. [Q5VYS8-1]
UniGeneiHs.597057.

Genome annotation databases

EnsembliENST00000375960; ENSP00000365127; ENSG00000083223. [Q5VYS8-4]
ENST00000375963; ENSP00000365130; ENSG00000083223. [Q5VYS8-1]
GeneIDi79670.
KEGGihsa:79670.
UCSCiuc004aoq.4. human. [Q5VYS8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTUT7_HUMAN
AccessioniPrimary (citable) accession number: Q5VYS8
Secondary accession number(s): Q5H9T0
, Q5VYS5, Q5VYS7, Q658Z9, Q659A2, Q6MZJ3, Q8N5F0, Q96N57, Q96NE8, Q9C0F2, Q9H8M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: December 7, 2004
Last modified: November 22, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families