Q5VYK3 (ECM29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome-associated protein ECM29 homolog
Short name=Ecm29

Gene names

Name:	ECM29
Synonyms:	KIAA0368

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1845 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis. Ref.6 Ref.7
Subunit structure	Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6. Ref.6 Ref.7
Subcellular location	Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm › cytoskeleton › centrosome. Nucleus. Endosome › multivesicular body. Cytoplasmic vesicle Ref.6 Ref.7.
Sequence similarities	Contains 27 HEAT repeats.
Sequence caution	The sequence AAH21127.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAH73240.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAH73465.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Endoplasmic reticulum Endosome Nucleus Proteasome
Coding sequence diversity	Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular_component	ER to Golgi transport vesicle Inferred from direct assay Ref.6. Source: UniProtKB centrosome Inferred from direct assay Ref.6. Source: UniProtKB early endosome Inferred from direct assay Ref.6. Source: UniProtKB endocytic vesicle Inferred from direct assay Ref.7. Source: UniProtKB endoplasmic reticulum Inferred from direct assay Ref.6. Source: UniProtKB endoplasmic reticulum-Golgi intermediate compartment Inferred from electronic annotation. Source: UniProtKB-SubCell multivesicular body Inferred from direct assay Ref.7. Source: UniProtKB nucleus Inferred from direct assay Ref.6. Source: UniProtKB proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1845	1845	Proteasome-associated protein ECM29 homolog		PRO_0000212559
Regions
	Repeat	34 – 71	38	HEAT 1
	Repeat	113 – 150	38	HEAT 2
	Repeat	168 – 211	44	HEAT 3
	Repeat	332 – 368	37	HEAT 4
	Repeat	393 – 432	40	HEAT 5
	Repeat	435 – 472	38	HEAT 6
	Repeat	475 – 513	39	HEAT 7
	Repeat	689 – 726	38	HEAT 8
	Repeat	727 – 765	39	HEAT 9
	Repeat	789 – 826	38	HEAT 10
	Repeat	835 – 874	40	HEAT 11
	Repeat	876 – 913	38	HEAT 12
	Repeat	937 – 975	39	HEAT 13
	Repeat	981 – 1018	38	HEAT 14
	Repeat	1019 – 1056	38	HEAT 15
	Repeat	1118 – 1155	38	HEAT 16
	Repeat	1158 – 1195	38	HEAT 17
	Repeat	1200 – 1237	38	HEAT 18
	Repeat	1249 – 1287	39	HEAT 19
	Repeat	1291 – 1329	39	HEAT 20
	Repeat	1354 – 1392	39	HEAT 21
	Repeat	1396 – 1433	38	HEAT 22
	Repeat	1523 – 1560	38	HEAT 23
	Repeat	1564 – 1601	38	HEAT 24
	Repeat	1611 – 1648	38	HEAT 25
	Repeat	1652 – 1689	38	HEAT 26
	Repeat	1785 – 1828	44	HEAT 27
Amino acid modifications
	Modified residue	830	1	Phosphoserine Ref.8
	Modified residue	836	1	Phosphothreonine Ref.10
Natural variations
	Natural variant	472	1	T → S. Corresponds to variant rs16916091 [ dbSNP | Ensembl ].		VAR_055702
Experimental info
	Sequence conflict	101	1	I → N in AI916718. Ref.2
	Sequence conflict	101	1	I → N in AK127247. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5VYK3 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 5809A9208F5B4441	FASTA	1,845	204,291
	10 20 30 40 50 60 MAAAAASASQ DELNQLERVF LRLGHAETDE QLQNIISKFL PPVLLKLSST QEGVRKKVME 70 80 90 100 110 120 LLVHLNKRIK SRPKIQLPVE TLLVQYQDPA AVSFVTNFTI IYVKMGYPRL PVEKQCELAP 130 140 150 160 170 180 TLLTAMEGKP QPQQDSLMHL LIPTLFHMKY PVESSKSASP FNLAEKPKTV QLLLDFMLDV 190 200 210 220 230 240 LLMPYGYVLN ESQSRQNSSS AQGSSSNSGG GSGIPQPPPG MSFYAAKRVI GDNPWTPEQL 250 260 270 280 290 300 EQCKLGIVKF IEAEQVPELE AVLHLVIASS DTRHSVATAA DLELKSKQSL IDWNNPAIIN 310 320 330 340 350 360 KMYKVYLGDI PLKTKEGAVL KPELKRDPVS TRVKLKIVPH LLRSRQAAET FPANIQVVYD 370 380 390 400 410 420 GLFGTNTNSK LRTLSLQFVH HICITCPEIK IKPLGPMLLN GLTKLINEYK EDPKLLSMAY 430 440 450 460 470 480 SAVGKLSSRM PHLFTKDIAL VQQLFEALCK EEPETRLAIQ EALSMMVGAY STLEGAQRTL 490 500 510 520 530 540 MEALVASYLI KPEVQVRQVA VKFASTVFPS DHIPSRYLLL LAAGDPREEV HGEAQRVLRC 550 560 570 580 590 600 LPGRNRKEST SEQMPSFPEM VYYIQEKASH RMKTPVKYMT GTTVLPFNPA AFGEIVLYLR 610 620 630 640 650 660 MCLAHSAGVV PTSQSLADMQ DHAPAIGRYI RTLMSSGQMA PSSSNKSGET NPVQIYIGLL 670 680 690 700 710 720 QQLLAGVGGL PVMYCLLEAV SVYPEKLATK FVDKTEWIKS LMNNSKEEMR ELAALFYSVV 730 740 750 760 770 780 VSTVSGNELK SMIEQLIKTT KDNHSPEIQH GSLLALGFTV GRYLAKKKMR MSEQQDLERN 790 800 810 820 830 840 ADTLPDQEEL IQSATETIGS FLDSTSPLLA IAACTALGEI GRNGPLPIPS EGSGFTKLHL 850 860 870 880 890 900 VESLLSRIPS SKETNKMKER AIQTLGYFPV GDGDFPHQKL LLQGLMDSVE AKQIELQFTI 910 920 930 940 950 960 GEAITSAAIG TSSVAARDAW QMTEEEYTPP AGAKVNDVVP WVLDVILNKH IISPNPHVRQ 970 980 990 1000 1010 1020 AACIWLLSLV RKLSTHKEVK SHLKEIQSAF VSVLSENDEL SQDVASKGLG LVYELGNEQD 1030 1040 1050 1060 1070 1080 QQELVSTLVE TLMTGKRVKH EVSGETVVFQ GGALGKTPDG QGLSTYKELC SLASDLSQPD 1090 1100 1110 1120 1130 1140 LVYKFMNLAN HHAMWNSRKG AAFGFNVIAT RAGEQLAPFL PQLVPRLYRY QFDPNLGIRQ 1150 1160 1170 1180 1190 1200 AMTSIWNALV TDKSMVDKYL KEILQDLVKN LTSNMWRVRE SSCLALNDLL RGRPLDDIID 1210 1220 1230 1240 1250 1260 KLPEIWETLF RVQDDIKESV RKAAELALKT LSKVCVKMCD PAKGAAGQRT IAALLPCLLD 1270 1280 1290 1300 1310 1320 KGMMSTVTEV RALSINTLVK ISKSAGAMLK PHAPKLIPAL LESLSVLEPQ VLNYLSLRAT 1330 1340 1350 1360 1370 1380 EQEKAAMDSA RLSAAKSSPM METINMCLQY LDVSVLGELV PRLCELIRSG VGLGTKGGCA 1390 1400 1410 1420 1430 1440 SVIVSLTTQC PQDLTPYSGK LMSALLSGLT DRNSVIQKSC AFAMGHLVRT SRDSSTEKLL 1450 1460 1470 1480 1490 1500 QKLNGWYMEK EEPIYKTSCA LTIHAIGRYS PDVLKNHAKE VLPLAFLGMH EIADEEKSEK 1510 1520 1530 1540 1550 1560 EECNLWTEVW QENVPGSFGG IRLYLQELIT ITQKALQSQS WKMKAQGAIA MASIAKQTSS 1570 1580 1590 1600 1610 1620 LVPPYLGMIL TALLQGLAGR TWAGKEELLK AIACVVTACS AELEKSVPNQ PSTNEILQAV 1630 1640 1650 1660 1670 1680 LKECSKENVK YKIVAISCAA DILKATKEDR FQEFSNIVIP LIKKNSLESS GVRTTKNEEE 1690 1700 1710 1720 1730 1740 NEKEKELQLE YLLGAFESLG KAWPRNAETQ RCYRQELCKL MCERLKLSTW KVQLGVLQSM 1750 1760 1770 1780 1790 1800 NAFFQGLMLL EEEHADPEAL AEILLETCKS ITYSLENKTY SSVRTEALSV IELLLKKLEE 1810 1820 1830 1840 SKQWECLTSE CRVLLIESLA TMEPDSRPEL QEKAALLKKT LENLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	The MGC Project Team Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-194.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 182-1845. Tissue: Hippocampus.
[4]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-1845. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1301-1845. Tissue: B-cell.
[6]	"Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles." Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M. J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components." Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M. J. Biol. Chem. 285:31616-31633(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS11; VPS26A; VPS36; RAB11FIP4; RABEP1; DCTN1; DCTN2; KIF5B; MYH7; MYH10; MYO10 AND ARF6.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-830, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-836, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL354661, AL159168 Genomic DNA. Translation: CAH73240.1. Sequence problems. AL159168, AL354661 Genomic DNA. Translation: CAH73465.1. Sequence problems. AI916718 mRNA. No translation available. AK127247 mRNA. No translation available. AB002366 mRNA. Translation: BAA20823.1. BC021127 mRNA. Translation: AAH21127.1. Different initiation.
IPI	IPI00157790.
UniGene	Hs.368255.
3D structure databases
ProteinModelPortal	Q5VYK3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5VYK3. 6 interactions.
MINT	MINT-1146313.
STRING	9606.ENSP00000259335.
PTM databases
PhosphoSite	Q5VYK3.
Polymorphism databases
DMDM	61212960.
Proteomic databases
PaxDb	Q5VYK3.
PRIDE	Q5VYK3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338205; ENSP00000339889; ENSG00000136813.
UCSC	uc004bfe.1. human.
Organism-specific databases
GeneCards	GC09M114122.
H-InvDB	HIX0008281.
HGNC	HGNC:29020. KIAA0368.
HPA	HPA021646.
neXtProt	NX_Q5VYK3.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250280.
HOGENOM	HOG000231642.
HOVERGEN	HBG051438.
InParanoid	Q5VYK3.
Gene expression databases
ArrayExpress	Q5VYK3.
Bgee	Q5VYK3.
CleanEx	HS_KIAA0368.
Genevestigator	Q5VYK3.
GermOnline	ENSG00000136813. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 6 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR026827. ECM29/GCN1. IPR026826. ECM29_metazoa. IPR024372. Proteasome_stabil_ECM29. [Graphical view]
PANTHER	PTHR23346. PTHR23346. 1 hit. PTHR23346:SF13. PTHR23346:SF13. 1 hit.
Pfam	PF13001. Ecm29. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 3 hits.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECM29_HUMAN

Accession

Primary (citable) accession number: Q5VYK3
Secondary accession number(s): O15074, Q8WU82

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.