Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5VYK3 (ECM29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome-associated protein ECM29 homolog

Short name=Ecm29
Gene names
Name:ECM29
Synonyms:KIAA0368
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1845 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis. Ref.6 Ref.7

Subunit structure

Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6. Ref.6 Ref.7

Subcellular location

Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus. Endosomemultivesicular body. Cytoplasmic vesicle Ref.6 Ref.7.

Sequence similarities

Contains 27 HEAT repeats.

Sequence caution

The sequence AAH21127.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAH73240.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAH73465.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 18451844Proteasome-associated protein ECM29 homolog
PRO_0000212559

Regions

Repeat34 – 7138HEAT 1
Repeat113 – 15038HEAT 2
Repeat168 – 21144HEAT 3
Repeat332 – 36837HEAT 4
Repeat393 – 43240HEAT 5
Repeat435 – 47238HEAT 6
Repeat475 – 51339HEAT 7
Repeat689 – 72638HEAT 8
Repeat727 – 76539HEAT 9
Repeat789 – 82638HEAT 10
Repeat835 – 87440HEAT 11
Repeat876 – 91338HEAT 12
Repeat937 – 97539HEAT 13
Repeat981 – 101838HEAT 14
Repeat1019 – 105638HEAT 15
Repeat1118 – 115538HEAT 16
Repeat1158 – 119538HEAT 17
Repeat1200 – 123738HEAT 18
Repeat1249 – 128739HEAT 19
Repeat1291 – 132939HEAT 20
Repeat1354 – 139239HEAT 21
Repeat1396 – 143338HEAT 22
Repeat1523 – 156038HEAT 23
Repeat1564 – 160138HEAT 24
Repeat1611 – 164838HEAT 25
Repeat1652 – 168938HEAT 26
Repeat1785 – 182844HEAT 27

Amino acid modifications

Modified residue21N-acetylalanine Ref.11 Ref.12
Modified residue8301Phosphoserine Ref.8
Modified residue8361Phosphothreonine Ref.10

Natural variations

Natural variant4721T → S.
Corresponds to variant rs16916091 [ dbSNP | Ensembl ].
VAR_055702

Experimental info

Sequence conflict1011I → N in AI916718. Ref.2
Sequence conflict1011I → N in AK127247. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5VYK3 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 5809A9208F5B4441

FASTA1,845204,291
        10         20         30         40         50         60 
MAAAAASASQ DELNQLERVF LRLGHAETDE QLQNIISKFL PPVLLKLSST QEGVRKKVME 

        70         80         90        100        110        120 
LLVHLNKRIK SRPKIQLPVE TLLVQYQDPA AVSFVTNFTI IYVKMGYPRL PVEKQCELAP 

       130        140        150        160        170        180 
TLLTAMEGKP QPQQDSLMHL LIPTLFHMKY PVESSKSASP FNLAEKPKTV QLLLDFMLDV 

       190        200        210        220        230        240 
LLMPYGYVLN ESQSRQNSSS AQGSSSNSGG GSGIPQPPPG MSFYAAKRVI GDNPWTPEQL 

       250        260        270        280        290        300 
EQCKLGIVKF IEAEQVPELE AVLHLVIASS DTRHSVATAA DLELKSKQSL IDWNNPAIIN 

       310        320        330        340        350        360 
KMYKVYLGDI PLKTKEGAVL KPELKRDPVS TRVKLKIVPH LLRSRQAAET FPANIQVVYD 

       370        380        390        400        410        420 
GLFGTNTNSK LRTLSLQFVH HICITCPEIK IKPLGPMLLN GLTKLINEYK EDPKLLSMAY 

       430        440        450        460        470        480 
SAVGKLSSRM PHLFTKDIAL VQQLFEALCK EEPETRLAIQ EALSMMVGAY STLEGAQRTL 

       490        500        510        520        530        540 
MEALVASYLI KPEVQVRQVA VKFASTVFPS DHIPSRYLLL LAAGDPREEV HGEAQRVLRC 

       550        560        570        580        590        600 
LPGRNRKEST SEQMPSFPEM VYYIQEKASH RMKTPVKYMT GTTVLPFNPA AFGEIVLYLR 

       610        620        630        640        650        660 
MCLAHSAGVV PTSQSLADMQ DHAPAIGRYI RTLMSSGQMA PSSSNKSGET NPVQIYIGLL 

       670        680        690        700        710        720 
QQLLAGVGGL PVMYCLLEAV SVYPEKLATK FVDKTEWIKS LMNNSKEEMR ELAALFYSVV 

       730        740        750        760        770        780 
VSTVSGNELK SMIEQLIKTT KDNHSPEIQH GSLLALGFTV GRYLAKKKMR MSEQQDLERN 

       790        800        810        820        830        840 
ADTLPDQEEL IQSATETIGS FLDSTSPLLA IAACTALGEI GRNGPLPIPS EGSGFTKLHL 

       850        860        870        880        890        900 
VESLLSRIPS SKETNKMKER AIQTLGYFPV GDGDFPHQKL LLQGLMDSVE AKQIELQFTI 

       910        920        930        940        950        960 
GEAITSAAIG TSSVAARDAW QMTEEEYTPP AGAKVNDVVP WVLDVILNKH IISPNPHVRQ 

       970        980        990       1000       1010       1020 
AACIWLLSLV RKLSTHKEVK SHLKEIQSAF VSVLSENDEL SQDVASKGLG LVYELGNEQD 

      1030       1040       1050       1060       1070       1080 
QQELVSTLVE TLMTGKRVKH EVSGETVVFQ GGALGKTPDG QGLSTYKELC SLASDLSQPD 

      1090       1100       1110       1120       1130       1140 
LVYKFMNLAN HHAMWNSRKG AAFGFNVIAT RAGEQLAPFL PQLVPRLYRY QFDPNLGIRQ 

      1150       1160       1170       1180       1190       1200 
AMTSIWNALV TDKSMVDKYL KEILQDLVKN LTSNMWRVRE SSCLALNDLL RGRPLDDIID 

      1210       1220       1230       1240       1250       1260 
KLPEIWETLF RVQDDIKESV RKAAELALKT LSKVCVKMCD PAKGAAGQRT IAALLPCLLD 

      1270       1280       1290       1300       1310       1320 
KGMMSTVTEV RALSINTLVK ISKSAGAMLK PHAPKLIPAL LESLSVLEPQ VLNYLSLRAT 

      1330       1340       1350       1360       1370       1380 
EQEKAAMDSA RLSAAKSSPM METINMCLQY LDVSVLGELV PRLCELIRSG VGLGTKGGCA 

      1390       1400       1410       1420       1430       1440 
SVIVSLTTQC PQDLTPYSGK LMSALLSGLT DRNSVIQKSC AFAMGHLVRT SRDSSTEKLL 

      1450       1460       1470       1480       1490       1500 
QKLNGWYMEK EEPIYKTSCA LTIHAIGRYS PDVLKNHAKE VLPLAFLGMH EIADEEKSEK 

      1510       1520       1530       1540       1550       1560 
EECNLWTEVW QENVPGSFGG IRLYLQELIT ITQKALQSQS WKMKAQGAIA MASIAKQTSS 

      1570       1580       1590       1600       1610       1620 
LVPPYLGMIL TALLQGLAGR TWAGKEELLK AIACVVTACS AELEKSVPNQ PSTNEILQAV 

      1630       1640       1650       1660       1670       1680 
LKECSKENVK YKIVAISCAA DILKATKEDR FQEFSNIVIP LIKKNSLESS GVRTTKNEEE 

      1690       1700       1710       1720       1730       1740 
NEKEKELQLE YLLGAFESLG KAWPRNAETQ RCYRQELCKL MCERLKLSTW KVQLGVLQSM 

      1750       1760       1770       1780       1790       1800 
NAFFQGLMLL EEEHADPEAL AEILLETCKS ITYSLENKTY SSVRTEALSV IELLLKKLEE 

      1810       1820       1830       1840 
SKQWECLTSE CRVLLIESLA TMEPDSRPEL QEKAALLKKT LENLE 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]The MGC Project Team
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-194.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 182-1845.
Tissue: Hippocampus.
[4]"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-1845.
Tissue: Brain.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1301-1845.
Tissue: B-cell.
[6]"Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles."
Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M.
J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components."
Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M.
J. Biol. Chem. 285:31616-31633(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS11; VPS26A; VPS36; RAB11FIP4; RABEP1; DCTN1; DCTN2; KIF5B; MYH7; MYH10; MYO10 AND ARF6.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-830, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-836, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL354661, AL159168 Genomic DNA. Translation: CAH73240.1. Sequence problems.
AL159168, AL354661 Genomic DNA. Translation: CAH73465.1. Sequence problems.
AI916718 mRNA. No translation available.
AK127247 mRNA. No translation available.
AB002366 mRNA. Translation: BAA20823.1.
BC021127 mRNA. Translation: AAH21127.1. Different initiation.
RefSeqXP_005251907.1. XM_005251850.2.
UniGeneHs.368255.

3D structure databases

ProteinModelPortalQ5VYK3.
SMRQ5VYK3. Positions 396-467, 1165-1304, 1376-1440.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ5VYK3. 8 interactions.
MINTMINT-1146313.
STRING9606.ENSP00000259335.

PTM databases

PhosphoSiteQ5VYK3.

Polymorphism databases

DMDM61212960.

Proteomic databases

MaxQBQ5VYK3.
PaxDbQ5VYK3.
PRIDEQ5VYK3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338205; ENSP00000339889; ENSG00000136813.
GeneID23392.
KEGGhsa:23392.

Organism-specific databases

CTD23392.
GeneCardsGC09M114122.
H-InvDBHIX0008281.
HGNCHGNC:29020. KIAA0368.
HPAHPA021646.
neXtProtNX_Q5VYK3.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250280.
HOGENOMHOG000231642.
HOVERGENHBG051438.
InParanoidQ5VYK3.
KOK11886.
OrthoDBEOG79SDW5.
PhylomeDBQ5VYK3.

Gene expression databases

ArrayExpressQ5VYK3.
BgeeQ5VYK3.
CleanExHS_KIAA0368.
GenevestigatorQ5VYK3.

Family and domain databases

Gene3D1.25.10.10. 6 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view]
PANTHERPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 7 hits.
ProtoNetSearch...

Other

GeneWikiKIAA0368.
GenomeRNAi23392.
PROQ5VYK3.

Entry information

Entry nameECM29_HUMAN
AccessionPrimary (citable) accession number: Q5VYK3
Secondary accession number(s): O15074, Q8WU82
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM