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Protein

KH domain-containing, RNA-binding, signal transduction-associated protein 2

Gene

KHDRBS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. May function as an adapter protein for Src kinases during mitosis. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) binding Source: Ensembl
  2. poly(U) RNA binding Source: Ensembl

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
KH domain-containing, RNA-binding, signal transduction-associated protein 2
Alternative name(s):
Sam68-like mammalian protein 1
Short name:
SLM-1
Short name:
hSLM-1
Gene namesi
Name:KHDRBS2
Synonyms:SLM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:18114. KHDRBS2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30093.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349KH domain-containing, RNA-binding, signal transduction-associated protein 2PRO_0000308953Add
BLAST

Post-translational modificationi

Methylated.1 Publication
Tyrosine phosphorylated by FYN, PTK6 and SRC. Tyrosine phosphorylated by SRC during mitosis (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ5VWX1.
PaxDbiQ5VWX1.
PRIDEiQ5VWX1.

PTM databases

PhosphoSiteiQ5VWX1.

Expressioni

Tissue specificityi

Highly expressed in brain, lung, kidney and small intestine. Weakly expressed in placenta, liver, spleen, thymus, ovary and colon.1 Publication

Gene expression databases

BgeeiQ5VWX1.
CleanExiHS_KHDRBS2.
GenevestigatoriQ5VWX1.

Organism-specific databases

HPAiHPA029392.

Interactioni

Subunit structurei

Self-associates to form homooligomers. Interacts with SAFB, SFRS9 and YTHDC1. Found in a complex with KHDRBS1, KHDRBS2 and KHDRBS3. Interacts with the SH3 domains of FYN and PLCG1. Interacts with the SH2 domains of FYN, GRAP2, PLCG1 and RASA1 (By similarity). Interacts with RBMX.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RBMXP381592EBI-742808,EBI-743526

Protein-protein interaction databases

BioGridi128436. 73 interactions.
IntActiQ5VWX1. 7 interactions.
MINTiMINT-1449914.
STRINGi9606.ENSP00000281156.

Structurei

3D structure databases

ProteinModelPortaliQ5VWX1.
SMRiQ5VWX1. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 13571KHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 26252Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the KHDRBS family.Curated
Contains 1 KH domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiCOG5176.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ5VWX1.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ5VWX1.
TreeFamiTF314878.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5VWX1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEEKYLPEL MAEKDSLDPS FVHASRLLAE EIEKFQGSDG KKEDEEKKYL
60 70 80 90 100
DVISNKNIKL SERVLIPVKQ YPKFNFVGKL LGPRGNSLKR LQEETGAKMS
110 120 130 140 150
ILGKGSMRDK AKEEELRKSG EAKYAHLSDE LHVLIEVFAP PGEAYSRMSH
160 170 180 190 200
ALEEIKKFLV PDYNDEIRQE QLRELSYLNG SEDSGRGRGI RGRGIRIAPT
210 220 230 240 250
APSRGRGGAI PPPPPPGRGV LTPRGSTVTR GALPVPPVAR GVPTPRARGA
260 270 280 290 300
PTVPGYRAPP PPAHEAYEEY GYDDGYGGEY DDQTYETYDN SYATQTQSVP
310 320 330 340
EYYDYGHGVS EDAYDSYAPE EWATTRSSLK APPQRSARGG YREHPYGRY
Length:349
Mass (Da):38,927
Last modified:December 7, 2004 - v1
Checksum:iEF16E301D8A12164
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → P in AAL77219. (PubMed:12549823)Curated
Sequence conflicti232 – 2321A → V in AAL77219. (PubMed:12549823)Curated
Sequence conflicti326 – 3261R → S in AAH34043. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti308 – 3081G → A.
Corresponds to variant rs7449840 [ dbSNP | Ensembl ].
VAR_036885

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077838 mRNA. Translation: AAL77219.1.
AK292043 mRNA. Translation: BAF84732.1.
AL360220
, AL049544, AL138882, AL355347, AL391644, Z93021 Genomic DNA. Translation: CAH70882.1.
AL355347
, AL049544, AL138882, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI14907.1.
AL391644
, AL049544, AL138882, AL355347, AL360220, Z93021 Genomic DNA. Translation: CAI15262.1.
AL138882
, AL049544, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19748.1.
AL049544
, AL138882, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19918.1.
Z93021
, AL049544, AL138882, AL355347, AL360220, AL391644 Genomic DNA. Translation: CAI21649.1.
CH471143 Genomic DNA. Translation: EAW88490.1.
BC034043 mRNA. Translation: AAH34043.1.
CCDSiCCDS4963.1.
RefSeqiNP_689901.2. NM_152688.2.
UniGeneiHs.519794.

Genome annotation databases

EnsembliENST00000281156; ENSP00000281156; ENSG00000112232.
GeneIDi202559.
KEGGihsa:202559.
UCSCiuc003peg.2. human.

Polymorphism databases

DMDMi74762274.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077838 mRNA. Translation: AAL77219.1.
AK292043 mRNA. Translation: BAF84732.1.
AL360220
, AL049544, AL138882, AL355347, AL391644, Z93021 Genomic DNA. Translation: CAH70882.1.
AL355347
, AL049544, AL138882, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI14907.1.
AL391644
, AL049544, AL138882, AL355347, AL360220, Z93021 Genomic DNA. Translation: CAI15262.1.
AL138882
, AL049544, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19748.1.
AL049544
, AL138882, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19918.1.
Z93021
, AL049544, AL138882, AL355347, AL360220, AL391644 Genomic DNA. Translation: CAI21649.1.
CH471143 Genomic DNA. Translation: EAW88490.1.
BC034043 mRNA. Translation: AAH34043.1.
CCDSiCCDS4963.1.
RefSeqiNP_689901.2. NM_152688.2.
UniGeneiHs.519794.

3D structure databases

ProteinModelPortaliQ5VWX1.
SMRiQ5VWX1. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128436. 73 interactions.
IntActiQ5VWX1. 7 interactions.
MINTiMINT-1449914.
STRINGi9606.ENSP00000281156.

PTM databases

PhosphoSiteiQ5VWX1.

Polymorphism databases

DMDMi74762274.

Proteomic databases

MaxQBiQ5VWX1.
PaxDbiQ5VWX1.
PRIDEiQ5VWX1.

Protocols and materials databases

DNASUi202559.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281156; ENSP00000281156; ENSG00000112232.
GeneIDi202559.
KEGGihsa:202559.
UCSCiuc003peg.2. human.

Organism-specific databases

CTDi202559.
GeneCardsiGC06M062447.
H-InvDBHIX0005989.
HGNCiHGNC:18114. KHDRBS2.
HPAiHPA029392.
MIMi610487. gene.
neXtProtiNX_Q5VWX1.
PharmGKBiPA30093.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5176.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ5VWX1.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ5VWX1.
TreeFamiTF314878.

Miscellaneous databases

GenomeRNAii202559.
NextBioi90301.
PROiQ5VWX1.
SOURCEiSearch...

Gene expression databases

BgeeiQ5VWX1.
CleanExiHS_KHDRBS2.
GenevestigatoriQ5VWX1.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel human STAR domain containing cDNA KHDRBS2."
    Wang L., Xu J., Zeng L., Ye X., Wu Q., Dai J., Ji C., Gu S., Zhao C., Xie Y., Mao Y.
    Mol. Biol. Rep. 29:369-375(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1."
    Cote J., Boisvert F.-M., Boulanger M.-C., Bedford M.T., Richard S.
    Mol. Biol. Cell 14:274-287(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION.
  7. "Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA."
    Heinrich B., Zhang Z., Raitskin O., Hiller M., Benderska N., Hartmann A.M., Bracco L., Elliott D., Ben-Ari S., Soreq H., Sperling J., Sperling R., Stamm S.
    J. Biol. Chem. 284:14303-14315(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBMX.

Entry informationi

Entry nameiKHDR2_HUMAN
AccessioniPrimary (citable) accession number: Q5VWX1
Secondary accession number(s): A8K7M8, Q8N4I4, Q8TCZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: February 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.