Q5VWX1 (KHDR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KH domain-containing, RNA-binding, signal transduction-associated protein 2

Alternative name(s):
Sam68-like mammalian protein 1
Short name=SLM-1
Short name=hSLM-1

Gene names

Name:	KHDRBS2
Synonyms:	SLM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. May function as an adapter protein for Src kinases during mitosis. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability By similarity.
Subunit structure	Self-associates to form homooligomers. Interacts with SAFB, SFRS9 and YTHDC1. Found in a complex with KHDRBS1, KHDRBS2 and KHDRBS3. Interacts with the SH3 domains of FYN and PLCG1. Interacts with the SH2 domains of FYN, GRAP2, PLCG1 and RASA1 By similarity. Interacts with RBMX. Ref.7
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in brain, lung, kidney and small intestine. Weakly expressed in placenta, liver, spleen, thymus, ovary and colon. Ref.1
Post-translational modification	Methylated. Ref.6 Tyrosine phosphorylated by FYN, PTK6 and SRC. Tyrosine phosphorylated by SRC during mitosis By similarity.
Sequence similarities	Belongs to the KHDRBS family. Contains 1 KH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	SH3-binding
Ligand	RNA-binding
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	poly(A) RNA binding Inferred from electronic annotation. Source: Compara poly(U) RNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RBMX	P38159	2	EBI-742808,EBI-743526

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	KH domain-containing, RNA-binding, signal transduction-associated protein 2		PRO_0000308953
Regions
	Domain	65 – 135	71	KH
	Compositional bias	211 – 262	52	Pro-rich
Natural variations
	Natural variant	308	1	G → A. Corresponds to variant rs7449840 [ dbSNP | Ensembl ].		VAR_036885
Experimental info
	Sequence conflict	65	1	L → P in AAL77219. Ref.1
	Sequence conflict	232	1	A → V in AAL77219. Ref.1
	Sequence conflict	326	1	R → S in AAH34043. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q5VWX1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: EF16E301D8A12164	FASTA	349	38,927
	10 20 30 40 50 60 MEEEKYLPEL MAEKDSLDPS FVHASRLLAE EIEKFQGSDG KKEDEEKKYL DVISNKNIKL 70 80 90 100 110 120 SERVLIPVKQ YPKFNFVGKL LGPRGNSLKR LQEETGAKMS ILGKGSMRDK AKEEELRKSG 130 140 150 160 170 180 EAKYAHLSDE LHVLIEVFAP PGEAYSRMSH ALEEIKKFLV PDYNDEIRQE QLRELSYLNG 190 200 210 220 230 240 SEDSGRGRGI RGRGIRIAPT APSRGRGGAI PPPPPPGRGV LTPRGSTVTR GALPVPPVAR 250 260 270 280 290 300 GVPTPRARGA PTVPGYRAPP PPAHEAYEEY GYDDGYGGEY DDQTYETYDN SYATQTQSVP 310 320 330 340 EYYDYGHGVS EDAYDSYAPE EWATTRSSLK APPQRSARGG YREHPYGRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel human STAR domain containing cDNA KHDRBS2." Wang L., Xu J., Zeng L., Ye X., Wu Q., Dai J., Ji C., Gu S., Zhao C., Xie Y., Mao Y. Mol. Biol. Rep. 29:369-375(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1." Cote J., Boisvert F.-M., Boulanger M.-C., Bedford M.T., Richard S. Mol. Biol. Cell 14:274-287(2003) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION.
[7]	"Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA." Heinrich B., Zhang Z., Raitskin O., Hiller M., Benderska N., Hartmann A.M., Bracco L., Elliott D., Ben-Ari S., Soreq H., Sperling J., Sperling R., Stamm S. J. Biol. Chem. 284:14303-14315(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RBMX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY077838 mRNA. Translation: AAL77219.1. AK292043 mRNA. Translation: BAF84732.1. AL360220 , AL049544, AL138882, AL355347, AL391644, Z93021 Genomic DNA. Translation: CAH70882.1. AL355347 , AL049544, AL138882, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI14907.1. AL391644 , AL049544, AL138882, AL355347, AL360220, Z93021 Genomic DNA. Translation: CAI15262.1. AL138882 , AL049544, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19748.1. AL049544 , AL138882, AL355347, AL360220, AL391644, Z93021 Genomic DNA. Translation: CAI19918.1. Z93021 , AL049544, AL138882, AL355347, AL360220, AL391644 Genomic DNA. Translation: CAI21649.1. CH471143 Genomic DNA. Translation: EAW88490.1. BC034043 mRNA. Translation: AAH34043.1.
IPI	IPI00166708.
RefSeq	NP_689901.2. NM_152688.2.
UniGene	Hs.519794.
3D structure databases
HSSP	HSSP built from PDB template 2BL5 based on UniProtKB Q32NN2.
ProteinModelPortal	Q5VWX1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5VWX1. 5 interactions.
MINT	MINT-1449914.
STRING	9606.ENSP00000281156.
PTM databases
PhosphoSite	Q5VWX1.
Polymorphism databases
DMDM	74762274.
Proteomic databases
PaxDb	Q5VWX1.
PRIDE	Q5VWX1.
Protocols and materials databases
DNASU	202559.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000281156; ENSP00000281156; ENSG00000112232.
GeneID	202559.
KEGG	hsa:202559.
UCSC	uc003peg.2. human.
Organism-specific databases
CTD	202559.
GeneCards	GC06M062447.
H-InvDB	HIX0005989.
HGNC	HGNC:18114. KHDRBS2.
HPA	HPA029392.
MIM	610487. gene.
neXtProt	NX_Q5VWX1.
PharmGKB	PA30093.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5176.
HOGENOM	HOG000230771.
HOVERGEN	HBG079164.
InParanoid	Q5VWX1.
OMA	PPIARGV.
OrthoDB	EOG418BP2.
PhylomeDB	Q5VWX1.
Gene expression databases
Bgee	Q5VWX1.
CleanEx	HS_KHDRBS2.
Genevestigator	Q5VWX1.
Family and domain databases
InterPro	IPR004087. KH_dom. IPR004088. KH_dom_type_1. [Graphical view]
Pfam	PF00013. KH_1. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	202559.
NextBio	90301.
SOURCE	Search...

Entry information

Entry name

KHDR2_HUMAN

Accession

Primary (citable) accession number: Q5VWX1
Secondary accession number(s): A8K7M8, Q8N4I4, Q8TCZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.