Q5VWQ0 (RSBN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Round spermatid basic protein 1

Gene names

Name:

RSBN1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylated by PKA By similarity.
Sequence similarities	Belongs to the round spermatid basic protein 1 family.
Sequence caution	The sequence AAH26155.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA92075.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB13974.1 differs from that shown. Reason: Erroneous initiation. The sequence CAH10493.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAH10493.1 differs from that shown. Reason: Aberrant splicing. The sequence CAH72507.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI19067.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5VWQ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 4 (identifier: Q5VWQ0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     460-802: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 802	802	Round spermatid basic protein 1		PRO_0000299412
Regions
	Motif	252 – 263	12	Nuclear localization signal Potential
	Compositional bias	103 – 165	63	Pro-rich
	Compositional bias	114 – 117	4	Poly-Arg
	Compositional bias	185 – 197	13	His-rich
	Compositional bias	252 – 261	10	Poly-Lys
Amino acid modifications
	Modified residue	81	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	460 – 802	343	Missing in isoform 4.		VSP_027656
Experimental info
	Sequence conflict	39 – 40	2	GP → SG in AAH26155. Ref.3
	Sequence conflict	106	1	E → G in BAF85241. Ref.1
	Sequence conflict	120	1	Q → R in BAA92075. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 78F229AB6A0FABD1	FASTA	802	90,072
	10 20 30 40 50 60 MFISGRRTAD KWRAEERLQC PAGSARAALA RCADGGAVGP FKCVFVGEMA AQVGAVRVVR 70 80 90 100 110 120 AVAAQEEPDK EGKEKPHAGV SPRGVKRQRR SSSGGSQEKR GRPSQEPPLA PPHRRRRSRQ 130 140 150 160 170 180 HPGPLPPTNA APTVPGPVEP LLLPPPPPPS LAPAGPAVAA PLPAPSTSAL FTFSPLTVSA 190 200 210 220 230 240 AGPKHKGHKE RHKHHHHRGP DGDPSSCGTD LKHKDKQENG ERTGGVPLIK APKRETPDEN 250 260 270 280 290 300 GKTQRADDFV LKKIKKKKKK KHREDMRGRR LKMYNKEVQT VCAGLTRISK EILTQGQINS 310 320 330 340 350 360 TSGLNKESFR YLKDEQLCRL NLGMQEYRVP QGVQTPFMTH QEHSIRRNFL KTGTKFSNFI 370 380 390 400 410 420 HEEHQSNGGA LVLHAYMDEL SFLSPMEMER FSEEFLALTF SENEKNAAYY ALAIVHGAAA 430 440 450 460 470 480 YLPDFLDYFA FNFPNTPVKM EILGKKDIET TTISNFHTQV NRTYCCGTYR AGPMRQISLV 490 500 510 520 530 540 GAVDEEVGDY FPEFLDMLEE SPFLKMTLPW GTLSSLRLQC RSQSDDGPIM WVRPGEQMIP 550 560 570 580 590 600 TADMPKSPFK RRRSMNEIKN LQYLPRTSEP REVLFEDRTR AHADHVGQGF DWQSTAAVGV 610 620 630 640 650 660 LKAVQFGEWS DQPRITKDVI CFHAEDFTDV VQRLQLDLHE PPVSQCVQWV DEAKLNQMRR 670 680 690 700 710 720 EGIRYARIQL CDNDIYFIPR NVIHQFKTVS AVCSLAWHIR LKQYHPVVEA TQNTESNSNM 730 740 750 760 770 780 DCGLTGKREL EVDSQCVRIK TESEEACTEI QLLTTASSSF PPASELNLQQ DQKTQPIPVL 790 800 KVESRLDSDQ QHNLQEHSTT SV
	Isoform 4 [UniParc]. Checksum: 9B1EA055DF7807A1	FASTA	459	50,802
	10 20 30 40 50 60 MFISGRRTAD KWRAEERLQC PAGSARAALA RCADGGAVGP FKCVFVGEMA AQVGAVRVVR 70 80 90 100 110 120 AVAAQEEPDK EGKEKPHAGV SPRGVKRQRR SSSGGSQEKR GRPSQEPPLA PPHRRRRSRQ 130 140 150 160 170 180 HPGPLPPTNA APTVPGPVEP LLLPPPPPPS LAPAGPAVAA PLPAPSTSAL FTFSPLTVSA 190 200 210 220 230 240 AGPKHKGHKE RHKHHHHRGP DGDPSSCGTD LKHKDKQENG ERTGGVPLIK APKRETPDEN 250 260 270 280 290 300 GKTQRADDFV LKKIKKKKKK KHREDMRGRR LKMYNKEVQT VCAGLTRISK EILTQGQINS 310 320 330 340 350 360 TSGLNKESFR YLKDEQLCRL NLGMQEYRVP QGVQTPFMTH QEHSIRRNFL KTGTKFSNFI 370 380 390 400 410 420 HEEHQSNGGA LVLHAYMDEL SFLSPMEMER FSEEFLALTF SENEKNAAYY ALAIVHGAAA 430 440 450 YLPDFLDYFA FNFPNTPVKM EILGKKDIET TTISNFHTQ

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo and Placenta.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-802 (ISOFORM 4). Tissue: Testis.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 47-802 (ISOFORM 1). Tissue: Liver tumor.
[5]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK002082 mRNA. Translation: BAA92075.1. Different initiation. AK292552 mRNA. Translation: BAF85241.1. AK022166 mRNA. Translation: BAB13974.1. Different initiation. AL365321, AL137856 Genomic DNA. Translation: CAH72507.1. Different initiation. AL137856, AL365321 Genomic DNA. Translation: CAI19067.1. Different initiation. BC026155 mRNA. Translation: AAH26155.1. Different initiation. CR627402 mRNA. Translation: CAH10493.1. Sequence problems.
IPI	IPI00019999. IPI00855810.
RefSeq	NP_060834.2. NM_018364.3.
UniGene	Hs.486285.
3D structure databases
ProteinModelPortal	Q5VWQ0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5VWQ0. 2 interactions.
STRING	9606.ENSP00000261441.
PTM databases
PhosphoSite	Q5VWQ0.
Polymorphism databases
DMDM	257050986.
Proteomic databases
PaxDb	Q5VWQ0.
PRIDE	Q5VWQ0.
Protocols and materials databases
DNASU	54665.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261441; ENSP00000261441; ENSG00000081019.
GeneID	54665.
KEGG	hsa:54665.
UCSC	uc001edq.3. human.
Organism-specific databases
CTD	54665.
GeneCards	GC01M114304.
H-InvDB	HIX0000902.
HGNC	HGNC:25642. RSBN1.
HPA	HPA042124.
neXtProt	NX_Q5VWQ0.
PharmGKB	PA134869532.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG306264.
HOGENOM	HOG000043117.
HOVERGEN	HBG094904.
InParanoid	Q5VWQ0.
OMA	LKHKDKQ.
OrthoDB	EOG4HT8RZ.
PhylomeDB	Q5VWQ0.
Gene expression databases
Bgee	Q5VWQ0.
CleanEx	HS_RSBN1.
Genevestigator	Q5VWQ0.
Family and domain databases
InterPro	IPR026306. RSBN1. [Graphical view]
PANTHER	PTHR13354. PTHR13354. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	54665.
NextBio	57216.

Entry information

Entry name

RSBN1_HUMAN

Accession

Primary (citable) accession number: Q5VWQ0
Secondary accession number(s): A8K937 , Q6AI21, Q8TC33, Q9HA80, Q9NUP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 1, 2009
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.