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Protein

Transcription initiation factor TFIID subunit 3

Gene

TAF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri865 – 915PHD-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ5VWG9.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 3
Alternative name(s):
140 kDa TATA box-binding protein-associated factor
TBP-associated factor 3
Transcription initiation factor TFIID 140 kDa subunit
Short name:
TAF(II)140
Short name:
TAF140
Short name:
TAFII-140
Short name:
TAFII140
Gene namesi
Name:TAF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17303. TAF3.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: HGNC
  • transcription factor TFIID complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23W → R: Loss of interaction with TAF10. 1 Publication1

Organism-specific databases

DisGeNETi83860.
OpenTargetsiENSG00000165632.
PharmGKBiPA38222.

Polymorphism and mutation databases

DMDMi74747393.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455281 – 929Transcription initiation factor TFIID subunit 3Add BLAST929

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1
Modified residuei266N6-acetyllysineCombined sources1
Modified residuei291PhosphoserineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei501PhosphothreonineCombined sources1
Modified residuei667PhosphoserineCombined sources1
Cross-linki746Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei755PhosphoserineCombined sources1
Modified residuei776N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5VWG9.
MaxQBiQ5VWG9.
PaxDbiQ5VWG9.
PeptideAtlasiQ5VWG9.
PRIDEiQ5VWG9.

PTM databases

iPTMnetiQ5VWG9.
PhosphoSitePlusiQ5VWG9.

Expressioni

Gene expression databases

BgeeiENSG00000165632.
CleanExiHS_TAF3.
GenevisibleiQ5VWG9. HS.

Interactioni

Subunit structurei

Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
H3F3BP842433EBI-1560087,EBI-120658

Protein-protein interaction databases

BioGridi123775. 27 interactors.
DIPiDIP-38920N.
IntActiQ5VWG9. 5 interactors.
STRINGi9606.ENSP00000340271.

Structurei

Secondary structure

1929
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi856 – 858Combined sources3
Beta strandi864 – 866Combined sources3
Turni869 – 871Combined sources3
Beta strandi880 – 882Combined sources3
Turni884 – 886Combined sources3
Beta strandi889 – 891Combined sources3
Helixi892 – 895Combined sources4
Turni909 – 911Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C13X-ray2.10A/C/E/G855-915[»]
ProteinModelPortaliQ5VWG9.
SMRiQ5VWG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 141Poly-Glu6
Compositional biasi163 – 168Poly-Glu6
Compositional biasi507 – 746Lys-richAdd BLAST240
Compositional biasi779 – 833Pro-richAdd BLAST55

Domaini

The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).By similarity

Sequence similaritiesi

Belongs to the TAF3 family.Curated
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri865 – 915PHD-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
KOG2389. Eukaryota.
ENOG410ZS4D. LUCA.
GeneTreeiENSGT00710000106806.
HOGENOMiHOG000231905.
HOVERGENiHBG083188.
InParanoidiQ5VWG9.
KOiK14650.
OMAiMPPNFPY.
OrthoDBiEOG091G0XX7.
PhylomeDBiQ5VWG9.
TreeFamiTF316513.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR006565. BTP.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5VWG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH
60 70 80 90 100
RYSELYGRTD PILDDVGEAF QLMGVSLHEL EDYIHNIEPV TFPHQIPSFP
110 120 130 140 150
VSKNNVLQFP QPGSKDAEER KEYIPDYLPP IVSSQEEEEE EQVPTDGGTS
160 170 180 190 200
AEAMQVPLEE DDELEEEEII NDENFLGKRP LDSPEAEELP AMKRPRLLST
210 220 230 240 250
KGDTLDVVLL EAREPLSSIN TQKIPPMLSP VHVQDSTDLA PPSPEPPMLA
260 270 280 290 300
PVAKSQMPTA KPLETKSFTP KTKTKTSSPG QKTKSPKTAQ SPAMVGSPIR
310 320 330 340 350
SPKTVSKEKK SPGRSKSPKS PKSPKVTTHI PQTPVRPETP NRTPSATLSE
360 370 380 390 400
KISKETIQVK QIQTPPDAGK LNSENQPKKA VVADKTIEAS IDAVIARACA
410 420 430 440 450
EREPDPFEFS SGSESEGDIF TSPKRISGPE CTTPKASTSA NNFTKSGSTP
460 470 480 490 500
LPLSGGTSSS DNSWTMDASI DEVVRKAKLG TPSNMPPNFP YISSPSVSPP
510 520 530 540 550
TPEPLHKVYE EKTKLPSSVE VKKKLKKELK TKMKKKEKQR DREREKDKNK
560 570 580 590 600
DKSKEKDKVK EKEKDKETGR ETKYPWKEFL KEEEADPYKF KIKEFEDVDP
610 620 630 640 650
KVKLKDGLVR KEKEKHKDKK KDREKGKKDK DKREKEKVKD KGREDKMKAP
660 670 680 690 700
APPLVLPPKE LALPLFSPAT ASRVPAMLPS LLPVLPEKLF EEKEKVKEKE
710 720 730 740 750
KKKDKKEKKK KKEKEKEKKE KEREKEKRER EKREKEKEKH KHEKIKVEPV
760 770 780 790 800
ALAPSPVIPR LTLRVGAGQD KIVISKVVPA PEAKPAPSQN RPKTPPPAPA
810 820 830 840 850
PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA KAPVRSVVTE
860 870 880 890 900
TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDWY HWPCVGIMTA
910 920
PPEEMQWFCP KCANKKKDKK HKKRKHRAH
Length:929
Mass (Da):103,582
Last modified:December 7, 2004 - v1
Checksum:iC951754B5B532E72
GO

Sequence cautioni

The sequence AAH45106 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH62352 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH73884 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23 – 25WDS → TRP in CAB56032 (PubMed:17974005).Curated3
Sequence conflicti533M → K in AAH73884 (PubMed:15489334).Curated1
Sequence conflicti537 – 539EKQ → KKK in AAH17320 (PubMed:15489334).Curated3
Sequence conflicti696V → P in AAH62352 (PubMed:15489334).Curated1
Sequence conflicti700E → K in CAB56032 (PubMed:17974005).Curated1
Sequence conflicti707E → K in AAH62352 (PubMed:15489334).Curated1
Sequence conflicti713E → K in AAH62352 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052254349S → T.Corresponds to variant rs17366712dbSNPEnsembl.1
Natural variantiVAR_052255442N → S.1 PublicationCorresponds to variant rs4747647dbSNPEnsembl.1
Natural variantiVAR_052256696V → A.1 PublicationCorresponds to variant rs1244229dbSNPEnsembl.1
Natural variantiVAR_052257696V → L.Corresponds to variant rs10795583dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292190 mRNA. Translation: CAC34475.1.
AL390294, AL159172, AL353754 Genomic DNA. Translation: CAH73142.1.
AL353754, AL159172, AL390294 Genomic DNA. Translation: CAH73451.1.
AL159172, AL353754, AL390294 Genomic DNA. Translation: CAI15661.1.
BC017320 mRNA. Translation: AAH17320.1.
BC045106 mRNA. Translation: AAH45106.1. Sequence problems.
BC073884 mRNA. Translation: AAH73884.1. Different initiation.
BC062352 mRNA. Translation: AAH62352.1. Different initiation.
AL117661 mRNA. Translation: CAB56032.1.
CCDSiCCDS41487.1.
PIRiT17342.
RefSeqiNP_114129.1. NM_031923.3.
UniGeneiHs.527688.
Hs.709995.

Genome annotation databases

EnsembliENST00000344293; ENSP00000340271; ENSG00000165632.
GeneIDi83860.
KEGGihsa:83860.
UCSCiuc010qbd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292190 mRNA. Translation: CAC34475.1.
AL390294, AL159172, AL353754 Genomic DNA. Translation: CAH73142.1.
AL353754, AL159172, AL390294 Genomic DNA. Translation: CAH73451.1.
AL159172, AL353754, AL390294 Genomic DNA. Translation: CAI15661.1.
BC017320 mRNA. Translation: AAH17320.1.
BC045106 mRNA. Translation: AAH45106.1. Sequence problems.
BC073884 mRNA. Translation: AAH73884.1. Different initiation.
BC062352 mRNA. Translation: AAH62352.1. Different initiation.
AL117661 mRNA. Translation: CAB56032.1.
CCDSiCCDS41487.1.
PIRiT17342.
RefSeqiNP_114129.1. NM_031923.3.
UniGeneiHs.527688.
Hs.709995.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C13X-ray2.10A/C/E/G855-915[»]
ProteinModelPortaliQ5VWG9.
SMRiQ5VWG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123775. 27 interactors.
DIPiDIP-38920N.
IntActiQ5VWG9. 5 interactors.
STRINGi9606.ENSP00000340271.

PTM databases

iPTMnetiQ5VWG9.
PhosphoSitePlusiQ5VWG9.

Polymorphism and mutation databases

DMDMi74747393.

Proteomic databases

EPDiQ5VWG9.
MaxQBiQ5VWG9.
PaxDbiQ5VWG9.
PeptideAtlasiQ5VWG9.
PRIDEiQ5VWG9.

Protocols and materials databases

DNASUi83860.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344293; ENSP00000340271; ENSG00000165632.
GeneIDi83860.
KEGGihsa:83860.
UCSCiuc010qbd.4. human.

Organism-specific databases

CTDi83860.
DisGeNETi83860.
GeneCardsiTAF3.
H-InvDBHIX0025228.
HIX0201494.
HGNCiHGNC:17303. TAF3.
MIMi606576. gene.
neXtProtiNX_Q5VWG9.
OpenTargetsiENSG00000165632.
PharmGKBiPA38222.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
KOG2389. Eukaryota.
ENOG410ZS4D. LUCA.
GeneTreeiENSGT00710000106806.
HOGENOMiHOG000231905.
HOVERGENiHBG083188.
InParanoidiQ5VWG9.
KOiK14650.
OMAiMPPNFPY.
OrthoDBiEOG091G0XX7.
PhylomeDBiQ5VWG9.
TreeFamiTF316513.

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ5VWG9.

Miscellaneous databases

GeneWikiiTAF3.
GenomeRNAii83860.
PROiQ5VWG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165632.
CleanExiHS_TAF3.
GenevisibleiQ5VWG9. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR006565. BTP.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAF3_HUMAN
AccessioniPrimary (citable) accession number: Q5VWG9
Secondary accession number(s): Q05DA0
, Q6GMS5, Q6P6B5, Q86VY6, Q9BQS9, Q9UFI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.