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Protein

Transcription initiation factor TFIID subunit 3

Gene

TAF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri865 – 915PHD-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORiQ5VWG9

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 3
Alternative name(s):
140 kDa TATA box-binding protein-associated factor
TBP-associated factor 3
Transcription initiation factor TFIID 140 kDa subunit
Short name:
TAF(II)140
Short name:
TAF140
Short name:
TAFII-140
Short name:
TAFII140
Gene namesi
Name:TAF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000165632.7
HGNCiHGNC:17303 TAF3
MIMi606576 gene
neXtProtiNX_Q5VWG9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23W → R: Loss of interaction with TAF10. 1 Publication1

Organism-specific databases

DisGeNETi83860
OpenTargetsiENSG00000165632
PharmGKBiPA38222

Polymorphism and mutation databases

DMDMi74747393

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455281 – 929Transcription initiation factor TFIID subunit 3Add BLAST929

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1
Modified residuei266N6-acetyllysineCombined sources1
Modified residuei291PhosphoserineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei501PhosphothreonineCombined sources1
Cross-linki581Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei667PhosphoserineCombined sources1
Cross-linki746Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei755PhosphoserineCombined sources1
Modified residuei776N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5VWG9
MaxQBiQ5VWG9
PaxDbiQ5VWG9
PeptideAtlasiQ5VWG9
PRIDEiQ5VWG9
ProteomicsDBi65526

PTM databases

iPTMnetiQ5VWG9
PhosphoSitePlusiQ5VWG9

Expressioni

Gene expression databases

BgeeiENSG00000165632
CleanExiHS_TAF3
GenevisibleiQ5VWG9 HS

Organism-specific databases

HPAiHPA066184

Interactioni

Subunit structurei

Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
H3F3BP842433EBI-1560087,EBI-120658

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123775, 29 interactors
ComplexPortaliCPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CORUMiQ5VWG9
DIPiDIP-38920N
IntActiQ5VWG9, 6 interactors
MINTiQ5VWG9
STRINGi9606.ENSP00000340271

Structurei

Secondary structure

1929
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi856 – 858Combined sources3
Beta strandi864 – 867Combined sources4
Turni869 – 871Combined sources3
Beta strandi880 – 882Combined sources3
Turni884 – 886Combined sources3
Beta strandi889 – 891Combined sources3
Helixi892 – 895Combined sources4
Helixi910 – 913Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C13X-ray2.10A/C/E/G855-915[»]
5WXGX-ray1.70A853-915[»]
5WXHX-ray1.30A/C853-915[»]
ProteinModelPortaliQ5VWG9
SMRiQ5VWG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 141Poly-Glu6
Compositional biasi163 – 168Poly-Glu6
Compositional biasi507 – 746Lys-richAdd BLAST240
Compositional biasi779 – 833Pro-richAdd BLAST55

Domaini

The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).By similarity

Sequence similaritiesi

Belongs to the TAF3 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri865 – 915PHD-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1973 Eukaryota
KOG2389 Eukaryota
ENOG410ZS4D LUCA
GeneTreeiENSGT00710000106806
HOGENOMiHOG000231905
HOVERGENiHBG083188
InParanoidiQ5VWG9
KOiK14650
OMAiMPPNFPY
OrthoDBiEOG091G0XX7
PhylomeDBiQ5VWG9
TreeFamiTF316513

Family and domain databases

Gene3Di1.10.20.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR006565 BTP
IPR009072 Histone-fold
IPR037815 TAF3
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12321:SF34 PTHR12321:SF34, 1 hit
PfamiView protein in Pfam
PF07524 Bromo_TP, 1 hit
PF00628 PHD, 1 hit
SMARTiView protein in SMART
SM00576 BTP, 1 hit
SM00249 PHD, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5VWG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH
60 70 80 90 100
RYSELYGRTD PILDDVGEAF QLMGVSLHEL EDYIHNIEPV TFPHQIPSFP
110 120 130 140 150
VSKNNVLQFP QPGSKDAEER KEYIPDYLPP IVSSQEEEEE EQVPTDGGTS
160 170 180 190 200
AEAMQVPLEE DDELEEEEII NDENFLGKRP LDSPEAEELP AMKRPRLLST
210 220 230 240 250
KGDTLDVVLL EAREPLSSIN TQKIPPMLSP VHVQDSTDLA PPSPEPPMLA
260 270 280 290 300
PVAKSQMPTA KPLETKSFTP KTKTKTSSPG QKTKSPKTAQ SPAMVGSPIR
310 320 330 340 350
SPKTVSKEKK SPGRSKSPKS PKSPKVTTHI PQTPVRPETP NRTPSATLSE
360 370 380 390 400
KISKETIQVK QIQTPPDAGK LNSENQPKKA VVADKTIEAS IDAVIARACA
410 420 430 440 450
EREPDPFEFS SGSESEGDIF TSPKRISGPE CTTPKASTSA NNFTKSGSTP
460 470 480 490 500
LPLSGGTSSS DNSWTMDASI DEVVRKAKLG TPSNMPPNFP YISSPSVSPP
510 520 530 540 550
TPEPLHKVYE EKTKLPSSVE VKKKLKKELK TKMKKKEKQR DREREKDKNK
560 570 580 590 600
DKSKEKDKVK EKEKDKETGR ETKYPWKEFL KEEEADPYKF KIKEFEDVDP
610 620 630 640 650
KVKLKDGLVR KEKEKHKDKK KDREKGKKDK DKREKEKVKD KGREDKMKAP
660 670 680 690 700
APPLVLPPKE LALPLFSPAT ASRVPAMLPS LLPVLPEKLF EEKEKVKEKE
710 720 730 740 750
KKKDKKEKKK KKEKEKEKKE KEREKEKRER EKREKEKEKH KHEKIKVEPV
760 770 780 790 800
ALAPSPVIPR LTLRVGAGQD KIVISKVVPA PEAKPAPSQN RPKTPPPAPA
810 820 830 840 850
PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA KAPVRSVVTE
860 870 880 890 900
TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDWY HWPCVGIMTA
910 920
PPEEMQWFCP KCANKKKDKK HKKRKHRAH
Length:929
Mass (Da):103,582
Last modified:December 7, 2004 - v1
Checksum:iC951754B5B532E72
GO

Sequence cautioni

The sequence AAH45106 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH62352 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH73884 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23 – 25WDS → TRP in CAB56032 (PubMed:17974005).Curated3
Sequence conflicti533M → K in AAH73884 (PubMed:15489334).Curated1
Sequence conflicti537 – 539EKQ → KKK in AAH17320 (PubMed:15489334).Curated3
Sequence conflicti696V → P in AAH62352 (PubMed:15489334).Curated1
Sequence conflicti700E → K in CAB56032 (PubMed:17974005).Curated1
Sequence conflicti707E → K in AAH62352 (PubMed:15489334).Curated1
Sequence conflicti713E → K in AAH62352 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052254349S → T. Corresponds to variant dbSNP:rs17366712Ensembl.1
Natural variantiVAR_052255442N → S1 PublicationCorresponds to variant dbSNP:rs4747647Ensembl.1
Natural variantiVAR_052256696V → A1 PublicationCorresponds to variant dbSNP:rs1244229Ensembl.1
Natural variantiVAR_052257696V → L. Corresponds to variant dbSNP:rs10795583Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292190 mRNA Translation: CAC34475.1
AL390294 Genomic DNA No translation available.
AL353754 Genomic DNA No translation available.
AL159172 Genomic DNA No translation available.
BC017320 mRNA Translation: AAH17320.1
BC045106 mRNA Translation: AAH45106.1 Sequence problems.
BC073884 mRNA Translation: AAH73884.1 Different initiation.
BC062352 mRNA Translation: AAH62352.1 Different initiation.
AL117661 mRNA Translation: CAB56032.1
CCDSiCCDS41487.1
PIRiT17342
RefSeqiNP_114129.1, NM_031923.3
UniGeneiHs.527688
Hs.709995

Genome annotation databases

EnsembliENST00000344293; ENSP00000340271; ENSG00000165632
GeneIDi83860
KEGGihsa:83860
UCSCiuc010qbd.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTAF3_HUMAN
AccessioniPrimary (citable) accession number: Q5VWG9
Secondary accession number(s): Q05DA0
, Q6GMS5, Q6P6B5, Q86VY6, Q9BQS9, Q9UFI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 7, 2004
Last modified: June 20, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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