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Protein

Ubiquitin-conjugating enzyme E2 U

Gene

UBE2U

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-like protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  1. DNA repair Source: GO_Central
  2. histone ubiquitination Source: GO_Central
  3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  4. protein polyubiquitination Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ5VVX9.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 U (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein U
Ubiquitin-protein ligase U
Gene namesi
Name:UBE2U
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:28559. UBE2U.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. HULC complex Source: GO_Central
  3. nuclear chromatin Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670656.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Ubiquitin-conjugating enzyme E2 UPRO_0000082512Add
BLAST

Proteomic databases

PaxDbiQ5VVX9.
PRIDEiQ5VVX9.

Expressioni

Gene expression databases

BgeeiQ5VVX9.
CleanExiHS_UBE2U.
ExpressionAtlasiQ5VVX9. baseline and differential.
GenevestigatoriQ5VVX9.

Organism-specific databases

HPAiHPA020472.
HPA021660.

Interactioni

Protein-protein interaction databases

BioGridi127156. 67 interactions.
IntActiQ5VVX9. 66 interactions.
STRINGi9606.ENSP00000360116.

Structurei

Secondary structure

1
321
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1916Combined sources
Beta strandi24 – 285Combined sources
Beta strandi35 – 417Combined sources
Turni47 – 504Combined sources
Beta strandi52 – 587Combined sources
Beta strandi69 – 746Combined sources
Turni83 – 853Combined sources
Helixi91 – 944Combined sources
Helixi96 – 983Combined sources
Helixi105 – 11612Combined sources
Helixi127 – 1348Combined sources
Helixi137 – 14711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YRVX-ray2.18A1-150[»]
ProteinModelPortaliQ5VVX9.
SMRiQ5VVX9. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5VVX9.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119350.
HOGENOMiHOG000049184.
HOVERGENiHBG080217.
InParanoidiQ5VVX9.
KOiK10584.
OrthoDBiEOG7G4QGZ.
PhylomeDBiQ5VVX9.
TreeFamiTF329302.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VVX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG
60 70 80 90 100
LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN
110 120 130 140 150
TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP
160 170 180 190 200
LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL
210 220 230 240 250
KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI
260 270 280 290 300
KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR
310 320
EEEVEDLISW TNTLNTNTSE D
Length:321
Mass (Da):37,741
Last modified:December 6, 2004 - v1
Checksum:i8104FE91620417BB
GO
Isoform 2 (identifier: Q5VVX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-321: Missing.

Show »
Length:226
Mass (Da):26,689
Checksum:i2DFF8434DD9452FB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti90 – 901I → T.
Corresponds to variant rs7532933 [ dbSNP | Ensembl ].
VAR_057323

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei227 – 32195Missing in isoform 2. 1 PublicationVSP_016003Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445205, AL161916 Genomic DNA. Translation: CAH71707.1.
AL445205, AL161916 Genomic DNA. Translation: CAH71708.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22119.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22120.1.
BC029895 mRNA. Translation: AAH29895.1.
CCDSiCCDS627.1. [Q5VVX9-2]
RefSeqiNP_689702.1. NM_152489.1. [Q5VVX9-2]
UniGeneiHs.388746.

Genome annotation databases

EnsembliENST00000371076; ENSP00000360116; ENSG00000177414. [Q5VVX9-2]
ENST00000611228; ENSP00000481174; ENSG00000177414. [Q5VVX9-1]
GeneIDi148581.
KEGGihsa:148581.
UCSCiuc001dbn.1. human. [Q5VVX9-1]

Polymorphism databases

DMDMi74747311.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445205, AL161916 Genomic DNA. Translation: CAH71707.1.
AL445205, AL161916 Genomic DNA. Translation: CAH71708.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22119.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22120.1.
BC029895 mRNA. Translation: AAH29895.1.
CCDSiCCDS627.1. [Q5VVX9-2]
RefSeqiNP_689702.1. NM_152489.1. [Q5VVX9-2]
UniGeneiHs.388746.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YRVX-ray2.18A1-150[»]
ProteinModelPortaliQ5VVX9.
SMRiQ5VVX9. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127156. 67 interactions.
IntActiQ5VVX9. 66 interactions.
STRINGi9606.ENSP00000360116.

Polymorphism databases

DMDMi74747311.

Proteomic databases

PaxDbiQ5VVX9.
PRIDEiQ5VVX9.

Protocols and materials databases

DNASUi148581.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371076; ENSP00000360116; ENSG00000177414. [Q5VVX9-2]
ENST00000611228; ENSP00000481174; ENSG00000177414. [Q5VVX9-1]
GeneIDi148581.
KEGGihsa:148581.
UCSCiuc001dbn.1. human. [Q5VVX9-1]

Organism-specific databases

CTDi148581.
GeneCardsiGC01P064669.
HGNCiHGNC:28559. UBE2U.
HPAiHPA020472.
HPA021660.
neXtProtiNX_Q5VVX9.
PharmGKBiPA142670656.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119350.
HOGENOMiHOG000049184.
HOVERGENiHBG080217.
InParanoidiQ5VVX9.
KOiK10584.
OrthoDBiEOG7G4QGZ.
PhylomeDBiQ5VVX9.
TreeFamiTF329302.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ5VVX9.

Miscellaneous databases

EvolutionaryTraceiQ5VVX9.
GenomeRNAii148581.
NextBioi85955.
PROiQ5VVX9.

Gene expression databases

BgeeiQ5VVX9.
CleanExiHS_UBE2U.
ExpressionAtlasiQ5VVX9. baseline and differential.
GenevestigatoriQ5VVX9.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Novel ubiquitin-conjugating enzyme."
    Avvakumov G.V., Walker J.R., Choe J., Newman E.M., MacKenzie F., Bochkarev A., Dhe-Paganon S.
    Submitted (JAN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.18 ANGSTROMS) OF 1-150.

Entry informationi

Entry nameiUBE2U_HUMAN
AccessioniPrimary (citable) accession number: Q5VVX9
Secondary accession number(s): Q8N1D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2005
Last sequence update: December 6, 2004
Last modified: March 31, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.