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Q5VVX9 (UBE2U_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 U

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein U
Ubiquitin-protein ligase U
Gene names
Name:UBE2U
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5VVX9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5VVX9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-321: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321Ubiquitin-conjugating enzyme E2 U
PRO_0000082512

Sites

Active site891Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence227 – 32195Missing in isoform 2.
VSP_016003
Natural variant901I → T.
Corresponds to variant rs7532933 [ dbSNP | Ensembl ].
VAR_057323

Secondary structure

......................... 321
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 8104FE91620417BB

FASTA32137,741
        10         20         30         40         50         60 
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG LVFQLTIHFT 

        70         80         90        100        110        120 
SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN TNYTLSSILL ALQVMLSNPV 

       130        140        150        160        170        180 
LENPVNLEAA RILVKDESLY RTILRLFNRP LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY 

       190        200        210        220        230        240 
QTWSRIATSK ATEYYRTPLL KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP 

       250        260        270        280        290        300 
HEVTHSMEEI KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR 

       310        320 
EEEVEDLISW TNTLNTNTSE D 

« Hide

Isoform 2 [UniParc].

Checksum: 2DFF8434DD9452FB
Show »

FASTA22626,689

References

« Hide 'large scale' references
[1]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Novel ubiquitin-conjugating enzyme."
Avvakumov G.V., Walker J.R., Choe J., Newman E.M., MacKenzie F., Bochkarev A., Dhe-Paganon S.
Submitted (FEB-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.18 ANGSTROMS) OF 1-150.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445205, AL161916 Genomic DNA. Translation: CAH71707.1.
AL445205, AL161916 Genomic DNA. Translation: CAH71708.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22119.1.
AL161916, AL445205 Genomic DNA. Translation: CAI22120.1.
BC029895 mRNA. Translation: AAH29895.1.
RefSeqNP_689702.1. NM_152489.1.
UniGeneHs.388746.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YRVX-ray2.18A1-150[»]
ProteinModelPortalQ5VVX9.
SMRQ5VVX9. Positions 1-177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127156. 65 interactions.
IntActQ5VVX9. 65 interactions.
STRING9606.ENSP00000360116.

Polymorphism databases

DMDM74747311.

Proteomic databases

PaxDbQ5VVX9.
PRIDEQ5VVX9.

Protocols and materials databases

DNASU148581.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371076; ENSP00000360116; ENSG00000177414. [Q5VVX9-2]
GeneID148581.
KEGGhsa:148581.
UCSCuc001dbn.1. human. [Q5VVX9-1]

Organism-specific databases

CTD148581.
GeneCardsGC01P064669.
HGNCHGNC:28559. UBE2U.
HPAHPA020472.
HPA021660.
neXtProtNX_Q5VVX9.
PharmGKBPA142670656.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000049184.
HOVERGENHBG080217.
InParanoidQ5VVX9.
KOK10584.
OMAHPNVDPH.
OrthoDBEOG7G4QGZ.
PhylomeDBQ5VVX9.
TreeFamTF329302.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkQ5VVX9.
UniPathwayUPA00143.

Gene expression databases

BgeeQ5VVX9.
CleanExHS_UBE2U.
GenevestigatorQ5VVX9.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5VVX9.
GenomeRNAi148581.
NextBio85955.
PROQ5VVX9.

Entry information

Entry nameUBE2U_HUMAN
AccessionPrimary (citable) accession number: Q5VVX9
Secondary accession number(s): Q8N1D4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM