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Q5VVX9

- UBE2U_HUMAN

UniProt

Q5VVX9 - UBE2U_HUMAN

Protein

Ubiquitin-conjugating enzyme E2 U

Gene

UBE2U

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins.PROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinkiQ5VVX9.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 U (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein U
    Ubiquitin-protein ligase U
    Gene namesi
    Name:UBE2U
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:28559. UBE2U.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142670656.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321Ubiquitin-conjugating enzyme E2 UPRO_0000082512Add
    BLAST

    Proteomic databases

    PaxDbiQ5VVX9.
    PRIDEiQ5VVX9.

    Expressioni

    Gene expression databases

    BgeeiQ5VVX9.
    CleanExiHS_UBE2U.
    GenevestigatoriQ5VVX9.

    Organism-specific databases

    HPAiHPA020472.
    HPA021660.

    Interactioni

    Protein-protein interaction databases

    BioGridi127156. 65 interactions.
    IntActiQ5VVX9. 66 interactions.
    STRINGi9606.ENSP00000360116.

    Structurei

    Secondary structure

    1
    321
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1916
    Beta strandi24 – 285
    Beta strandi35 – 417
    Turni47 – 504
    Beta strandi52 – 587
    Beta strandi69 – 746
    Turni83 – 853
    Helixi91 – 944
    Helixi96 – 983
    Helixi105 – 11612
    Helixi127 – 1348
    Helixi137 – 14711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YRVX-ray2.18A1-150[»]
    ProteinModelPortaliQ5VVX9.
    SMRiQ5VVX9. Positions 1-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5VVX9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000049184.
    HOVERGENiHBG080217.
    InParanoidiQ5VVX9.
    KOiK10584.
    OMAiHPNVDPH.
    OrthoDBiEOG7G4QGZ.
    PhylomeDBiQ5VVX9.
    TreeFamiTF329302.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5VVX9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG    50
    LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN 100
    TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP 150
    LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL 200
    KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI 250
    KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR 300
    EEEVEDLISW TNTLNTNTSE D 321
    Length:321
    Mass (Da):37,741
    Last modified:December 7, 2004 - v1
    Checksum:i8104FE91620417BB
    GO
    Isoform 2 (identifier: Q5VVX9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         227-321: Missing.

    Show »
    Length:226
    Mass (Da):26,689
    Checksum:i2DFF8434DD9452FB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti90 – 901I → T.
    Corresponds to variant rs7532933 [ dbSNP | Ensembl ].
    VAR_057323

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei227 – 32195Missing in isoform 2. 1 PublicationVSP_016003Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445205, AL161916 Genomic DNA. Translation: CAH71707.1.
    AL445205, AL161916 Genomic DNA. Translation: CAH71708.1.
    AL161916, AL445205 Genomic DNA. Translation: CAI22119.1.
    AL161916, AL445205 Genomic DNA. Translation: CAI22120.1.
    BC029895 mRNA. Translation: AAH29895.1.
    CCDSiCCDS627.1. [Q5VVX9-2]
    RefSeqiNP_689702.1. NM_152489.1. [Q5VVX9-2]
    UniGeneiHs.388746.

    Genome annotation databases

    EnsembliENST00000371076; ENSP00000360116; ENSG00000177414. [Q5VVX9-2]
    GeneIDi148581.
    KEGGihsa:148581.
    UCSCiuc001dbn.1. human. [Q5VVX9-1]

    Polymorphism databases

    DMDMi74747311.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445205 , AL161916 Genomic DNA. Translation: CAH71707.1 .
    AL445205 , AL161916 Genomic DNA. Translation: CAH71708.1 .
    AL161916 , AL445205 Genomic DNA. Translation: CAI22119.1 .
    AL161916 , AL445205 Genomic DNA. Translation: CAI22120.1 .
    BC029895 mRNA. Translation: AAH29895.1 .
    CCDSi CCDS627.1. [Q5VVX9-2 ]
    RefSeqi NP_689702.1. NM_152489.1. [Q5VVX9-2 ]
    UniGenei Hs.388746.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YRV X-ray 2.18 A 1-150 [» ]
    ProteinModelPortali Q5VVX9.
    SMRi Q5VVX9. Positions 1-148.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127156. 65 interactions.
    IntActi Q5VVX9. 66 interactions.
    STRINGi 9606.ENSP00000360116.

    Polymorphism databases

    DMDMi 74747311.

    Proteomic databases

    PaxDbi Q5VVX9.
    PRIDEi Q5VVX9.

    Protocols and materials databases

    DNASUi 148581.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000371076 ; ENSP00000360116 ; ENSG00000177414 . [Q5VVX9-2 ]
    GeneIDi 148581.
    KEGGi hsa:148581.
    UCSCi uc001dbn.1. human. [Q5VVX9-1 ]

    Organism-specific databases

    CTDi 148581.
    GeneCardsi GC01P064669.
    HGNCi HGNC:28559. UBE2U.
    HPAi HPA020472.
    HPA021660.
    neXtProti NX_Q5VVX9.
    PharmGKBi PA142670656.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000049184.
    HOVERGENi HBG080217.
    InParanoidi Q5VVX9.
    KOi K10584.
    OMAi HPNVDPH.
    OrthoDBi EOG7G4QGZ.
    PhylomeDBi Q5VVX9.
    TreeFami TF329302.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinki Q5VVX9.

    Miscellaneous databases

    EvolutionaryTracei Q5VVX9.
    GenomeRNAii 148581.
    NextBioi 85955.
    PROi Q5VVX9.

    Gene expression databases

    Bgeei Q5VVX9.
    CleanExi HS_UBE2U.
    Genevestigatori Q5VVX9.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    3. "Novel ubiquitin-conjugating enzyme."
      Avvakumov G.V., Walker J.R., Choe J., Newman E.M., MacKenzie F., Bochkarev A., Dhe-Paganon S.
      Submitted (FEB-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.18 ANGSTROMS) OF 1-150.

    Entry informationi

    Entry nameiUBE2U_HUMAN
    AccessioniPrimary (citable) accession number: Q5VVX9
    Secondary accession number(s): Q8N1D4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3