Reviewed, UniProtKB/Swiss-Prot Q5VV41 (ARHGG_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho guanine nucleotide exchange factor 16

Gene names

Name:	ARHGEF16
Synonyms:	NBR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	SH3 domain
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5VV41-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5VV41-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-288: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 709

709

Rho guanine nucleotide exchange factor 16

PRO_0000233690

Regions

Domain

284 – 468

185

DH

Domain

501 – 620

120

PH

Domain

629 – 689

61

SH3

Amino acid modifications

Modified residue

41

1

Phosphoserine

Modified residue

107

1

Phosphoserine

Modified residue

174

1

Phosphoserine

Modified residue

208

1

Phosphoserine

Modified residue

227

1

Phosphoserine

Modified residue

230

1

Phosphoserine

Modified residue

240

1

Phosphoserine

Modified residue

577

1

Phosphoserine

Natural variations

Alternative sequence

1 – 288

288

Missing in isoform 2.

VSP_018149

Experimental info

Sequence conflict

157 – 158

2

AM → RG in AAH02681. Ref.4

Sequence conflict

370

1

H → Y in AAH02681. Ref.1 Ref.2 Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

709

Helix Strand Turn

Details...

Beta strand

633 – 638

6

Beta strand

644 – 647

4

Beta strand

654 – 662

9

Beta strand

665 – 670

6

Turn

671 – 673

3

Beta strand

676 – 679

4

Helix

681 – 683

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 9672AB3C92311BEB	FASTA	709	80,105
	10 20 30 40 50 60 MAQRHSDSSL EEKLLGHRFH SELRLDAGGN PASGLPMVRG SPRVRDDAAF QPQVPAPPQP 70 80 90 100 110 120 RPPGHEEPWP IVLSTESPAA LKLGTQQLIP KSLAVASKAK TPARHQSFGA AVLSREAARR 130 140 150 160 170 180 DPKLLPAPSF SLDDMDVDKD PGGMLRRNLR NQSYRAAMKG LGKPGGQGDA IQLSPKLQAL 190 200 210 220 230 240 AEEPSQPHTR SPAKNKKTLG RKRGHKGSFK DDPQLYQEIQ ERGLNTSQES DDDILDESSS 250 260 270 280 290 300 PEGTQKVDAT IVVKSYRPAQ VTWSQLPEVV ELGILDQLST EERKRQEAMF EILTSEFSYQ 310 320 330 340 350 360 HSLSILVEEF LQSKELRATV TQMEHHHLFS NILDVLGASQ RFFEDLEQRH KAQVLVEDIS 370 380 390 400 410 420 DILEEHAEKH FHPYIAYCSN EVYQQRTLQK LISSNAAFRE ALREIERRPA CGGLPMLSFL 430 440 450 460 470 480 ILPMQRVTRL PLLMDTLCLK TQGHSERYKA ASRALKAISK LVRQCNEGAH RMERMEQMYT 490 500 510 520 530 540 LHTQLDFSKV KSLPLISASR WLLKRGELFL VEETGLFRKI ASRPTCYLFL FNDVLVVTKK 550 560 570 580 590 600 KSEESYMVQD YAQMNHIQVE KIEPSELPLP GGGNRSSSVP HPFQVTLLRN SEGRQEQLLL 610 620 630 640 650 660 SSDSASDRAR WIVALTHSER QWQGLSSKGD LPQVEITKAF FAKQADEVTL QQADVVLVLQ 670 680 690 700 QEDGWLYGER LRDGETGWFP EDFARFITSR VAVEGNVRRM ERLRVETDV
	Isoform 2 [UniParc]. Checksum: 7A75C46922C40EA4		421	48,629
	10 20 30 40 50 60 MFEILTSEFS YQHSLSILVE EFLQSKELRA TVTQMEHHHL FSNILDVLGA SQRFFEDLEQ 70 80 90 100 110 120 RHKAQVLVED ISDILEEHAE KHFHPYIAYC SNEVYQQRTL QKLISSNAAF REALREIERR 130 140 150 160 170 180 PACGGLPMLS FLILPMQRVT RLPLLMDTLC LKTQGHSERY KAASRALKAI SKLVRQCNEG 190 200 210 220 230 240 AHRMERMEQM YTLHTQLDFS KVKSLPLISA SRWLLKRGEL FLVEETGLFR KIASRPTCYL 250 260 270 280 290 300 FLFNDVLVVT KKKSEESYMV QDYAQMNHIQ VEKIEPSELP LPGGGNRSSS VPHPFQVTLL 310 320 330 340 350 360 RNSEGRQEQL LLSSDSASDR ARWIVALTHS ERQWQGLSSK GDLPQVEITK AFFAKQADEV 370 380 390 400 410 420 TLQQADVVLV LQQEDGWLYG ERLRDGETGW FPEDFARFIT SRVAVEGNVR RMERLRVETD V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a candidate gene for human neuroblastoma mapped to 1p36.3, NBR: a new member of the Rho/Rac GEF family." Sasaki S., Takei Y., Ito M., Nakagawara A., Fujiwara T., Takahashi E., Muto T., Tokino T., Nakamura Y. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-709. Tissue: PNS.
[5]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, MASS SPECTROMETRY.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; SER-107; SER-174; SER-208; SER-227; SER-230; SER-240 AND SER-577, MASS SPECTROMETRY.
[7]	"Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: STRUCTURE BY NMR OF 622-687.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D89016 mRNA. Translation: BAA13745.1.
BT007270 mRNA. Translation: AAP35934.1.
AL512413 Genomic DNA. Translation: CAH70832.1.
AL512413 Genomic DNA. Translation: CAH70833.1.
BC002681 mRNA. Translation: AAH02681.1. Different initiation.
BC051838 mRNA. Translation: AAH51838.1.

RefSeq

NP_055263.2.

UniGene

Hs.87435

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X6B	NMR	-	A	622-687	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q5VV41.

PTM databases

PhosphoSite

Q5VV41.

Genome annotation databases

Ensembl

ENSG00000130762. Homo sapiens. [Contig view]

GeneID

27237.

KEGG

hsa:27237.

Organism-specific databases

HGNC

HGNC:15515. ARHGEF16.

HPA

HPA010609.

PharmGKB

PA24971.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q5VV41.

HOVERGEN

Q5VV41.

Enzyme and pathway databases

Reactome

REACT_11044. Signaling by Rho GTPases.

Gene expression databases

ArrayExpress

Q5VV41.

CleanEx

HS_ARHGEF16.

GermOnline

ENSG00000130762. Homo sapiens.

Family and domain databases

InterPro

IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001849. PH.
IPR001452. SH3.
[Graphical view]

Gene3D

G3DSA:1.20.900.10. RhoGEF. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00452. SH3DOMAIN.

ProDom

PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

PROSITE

PS00741. DH_1. False negative.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

50111.

Entry information

Entry name

ARHGG_HUMAN

Accession

Primary (citable) accession number: Q5VV41
Secondary accession number(s): Q86TF0, Q99434

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	December 7, 2004
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)