Q5VU57 (CBPC6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic carboxypeptidase 6
EC=3.4.17.-

Alternative name(s):
ATP/GTP-binding protein-like 4

Gene names

Name:	AGBL4
Synonyms:	CCP6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with MYLK By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the peptidase M14 family.
Sequence caution	The sequence BAG52938.1 differs from that shown. Reason: Frameshift at position 501. The sequence BAG57648.1 differs from that shown. Reason: Frameshift at position 501.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C-terminal protein deglutamylation Inferred from sequence or structural similarity. Source: UniProtKB protein side chain deglutamylation Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metallocarboxypeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5VU57-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5VU57-2)

The sequence of this isoform differs from the canonical sequence as follows:
     94-94: Q → QVREIVDTFRVVL
     455-463: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Cytosolic carboxypeptidase 6		PRO_0000284835
Sites
	Active site	280	1	Nucleophile By similarity
	Metal binding	230	1	Zinc By similarity
	Metal binding	233	1	Zinc By similarity
	Metal binding	328	1	Zinc By similarity
Natural variations
	Alternative sequence	94	1	Q → QVREIVDTFRVVL in isoform 2.		VSP_040435
	Alternative sequence	455 – 463	9	Missing in isoform 2.		VSP_040436
	Natural variant	443	1	V → M. Corresponds to variant rs60977321 [ dbSNP | Ensembl ].		VAR_061078
Experimental info
	Sequence conflict	237	1	S → L in BAG57648. Ref.1
	Sequence conflict	490	1	K → R in BAG57648. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 4D1AD20229664D90	FASTA	503	58,230
	10 20 30 40 50 60 MAEGSQSAPE AGNDMGNDDA IGGNVSKYIV LPTGYCGQPK KGHLIFDACF ESGNLGRVDQ 70 80 90 100 110 120 VSEFEYDLFI RPDTCNPRFR VWFNFTVENV KESQRVIFNI VNFSKTKSLY RDGMAPMVKS 130 140 150 160 170 180 TSRPKWQRLP PKNVYYYRCP DHRKNYVMSF AFCFDREEDI YQFAYCYPYT YTRFQHYLDS 190 200 210 220 230 240 LQKRNMDYFF REQLGQSVQQ RKLDLLTITS PDNLREGAEQ KVVFITGRVH PGETPSSFVC 250 260 270 280 290 300 QGIIDFLVSQ HPIACVLREY LVFKIAPMLN PDGVYLGNYR CSLMGFDLNR HWLDPSPWVH 310 320 330 340 350 360 PTLHGVKQLI VQMYNDPKTS LEFYIDIHAH STMMNGFMYG NIFEDEERFQ RQAIFPKLLC 370 380 390 400 410 420 QNAEDFSYSS TSFNRDAVKA GTGRRFLGGL LDHTSYCYTL EVSFYSYIIS GTTAAVPYTE 430 440 450 460 470 480 EAYMKLGRNV ARTFLDYYRL NPVVEKVAIP MPRLRNKEIE VQRRKEKSPP YKHPLLRGPA 490 500 SNYPNSKGDK KSSVNHKDPS TPF
	Isoform 2 [UniParc]. Checksum: 20C9AED5564F23F9	FASTA	506	58,505
	10 20 30 40 50 60 MAEGSQSAPE AGNDMGNDDA IGGNVSKYIV LPTGYCGQPK KGHLIFDACF ESGNLGRVDQ 70 80 90 100 110 120 VSEFEYDLFI RPDTCNPRFR VWFNFTVENV KESQVREIVD TFRVVLRVIF NIVNFSKTKS 130 140 150 160 170 180 LYRDGMAPMV KSTSRPKWQR LPPKNVYYYR CPDHRKNYVM SFAFCFDREE DIYQFAYCYP 190 200 210 220 230 240 YTYTRFQHYL DSLQKRNMDY FFREQLGQSV QQRKLDLLTI TSPDNLREGA EQKVVFITGR 250 260 270 280 290 300 VHPGETPSSF VCQGIIDFLV SQHPIACVLR EYLVFKIAPM LNPDGVYLGN YRCSLMGFDL 310 320 330 340 350 360 NRHWLDPSPW VHPTLHGVKQ LIVQMYNDPK TSLEFYIDIH AHSTMMNGFM YGNIFEDEER 370 380 390 400 410 420 FQRQAIFPKL LCQNAEDFSY SSTSFNRDAV KAGTGRRFLG GLLDHTSYCY TLEVSFYSYI 430 440 450 460 470 480 ISGTTAAVPY TEEAYMKLGR NVARTFLDYY RLNPVVEKVA IPMPRLRKEK SPPYKHPLLR 490 500 GPASNYPNSK GDKKSSVNHK DPSTPF

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Amygdala and Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK094826 mRNA. Translation: BAG52938.1. Frameshift. AK294394 mRNA. Translation: BAG57648.1. Frameshift. AL590432 , AC099788, AL355809, AL390023, AL391844, AL591602, AL645507, AL645730 Genomic DNA. Translation: CAH70510.1. AL391844 , AC099788, AL355809, AL390023, AL590432, AL591602, AL645507, AL645730 Genomic DNA. Translation: CAI13521.1. AL645507 , AC099788, AL355809, AL390023, AL391844, AL590432, AL591602, AL645730 Genomic DNA. Translation: CAI14372.1. AL645730 , AC099788, AL355809, AL390023, AL391844, AL590432, AL591602, AL645507 Genomic DNA. Translation: CAI17069.1. AL591602 , AC099788, AL355809, AL390023, AL391844, AL590432, AL645507, AL645730 Genomic DNA. Translation: CAI19137.1. AL355809 , AC099788, AL390023, AL391844, AL590432, AL591602, AL645507, AL645730 Genomic DNA. Translation: CAI22167.1. AL390023 , AC099788, AL355809, AL391844, AL590432, AL591602, AL645507, AL645730 Genomic DNA. Translation: CAI23365.1.
IPI	IPI00973592. IPI01013035.
RefSeq	NP_116174.3. NM_032785.3.
UniGene	Hs.551844. Hs.679809.
3D structure databases
ProteinModelPortal	Q5VU57.
SMR	Q5VU57. Positions 48-386.
ModBase	Search...
Protein family/group databases
MEROPS	M14.027.
PTM databases
PhosphoSite	Q5VU57.
Polymorphism databases
DMDM	145558874.
Proteomic databases
PaxDb	Q5VU57.
PRIDE	Q5VU57.
Protocols and materials databases
DNASU	84871.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371839; ENSP00000360905; ENSG00000186094.
GeneID	84871.
KEGG	hsa:84871.
UCSC	uc001cru.2. human. uc010omx.1. human.
Organism-specific databases
CTD	84871.
GeneCards	GC01M048998.
HGNC	HGNC:25892. AGBL4.
HPA	HPA037994.
neXtProt	NX_Q5VU57.
PharmGKB	PA142672636.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2866.
HOGENOM	HOG000231164.
HOVERGEN	HBG107588.
InParanoid	Q5VU57.
OrthoDB	EOG45X7X0.
Gene expression databases
ArrayExpress	Q5VU57.
Bgee	Q5VU57.
CleanEx	HS_AGBL4.
Genevestigator	Q5VU57.
Family and domain databases
InterPro	IPR000834. Peptidase_M14. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AGBL4. human.
GenomeRNAi	84871.
NextBio	75159.

Entry information

Entry name

CBPC6_HUMAN

Accession

Primary (citable) accession number: Q5VU57
Secondary accession number(s): B3KT26, B4DG37

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.