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Q5VTL8 (PR38B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor 38B
Alternative name(s):
Sarcoma antigen NY-SAR-27
Gene names
Name:PRPF38B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be required for pre-mRNA splicing Potential.

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the PRP38 family.

Sequence caution

The sequence AAO65168.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAH72070.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI12934.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentspliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5VTL8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5VTL8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     187-190: DLDV → FFTL
     191-546: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 546545Pre-mRNA-splicing factor 38B
PRO_0000287235

Regions

Coiled coil292 – 32130 Potential
Compositional bias253 – 451199Arg-rich

Amino acid modifications

Modified residue21N-acetylalanine Ref.8
Modified residue2271N6-acetyllysine Ref.9
Modified residue3201Phosphoserine Ref.11
Modified residue4481Phosphoserine Ref.10
Modified residue4731Phosphoserine Ref.11
Modified residue4751Phosphoserine Ref.11
Modified residue4811Phosphoserine Ref.11
Modified residue5271Phosphoserine Ref.6 Ref.7 Ref.10 Ref.11
Modified residue5291Phosphoserine Ref.6 Ref.7 Ref.10 Ref.11
Modified residue5341Phosphoserine Ref.7 Ref.10

Natural variations

Alternative sequence187 – 1904DLDV → FFTL in isoform 2.
VSP_025408
Alternative sequence191 – 546356Missing in isoform 2.
VSP_025409

Experimental info

Sequence conflict521N → S in BAA91546. Ref.1
Sequence conflict294 – 2952EL → DR in AAH40127. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: A3082DCF491F88F8

FASTA54664,468
        10         20         30         40         50         60 
MANNSPALTG NSQPQHQAAA AAAQQQQQCG GGGATKPAVS GKQGNVLPLW GNEKTMNLNP 

        70         80         90        100        110        120 
MILTNILSSP YFKVQLYELK TYHEVVDEIY FKVTHVEPWE KGSRKTAGQT GMCGGVRGVG 

       130        140        150        160        170        180 
TGGIVSTAFC LLYKLFTLKL TRKQVMGLIT HTDSPYIRAL GFMYIRYTQP PTDLWDWFES 

       190        200        210        220        230        240 
FLDDEEDLDV KAGGGCVMTI GEMLRSFLTK LEWFSTLFPR IPVPVQKNID QQIKTRPRKI 

       250        260        270        280        290        300 
KKDGKEGAEE IDRHVERRRS RSPRRSLSPR RSPRRSRSRS HHREGHGSSS FDRELEREKE 

       310        320        330        340        350        360 
RQRLEREAKE REKERRRSRS IDRGLERRRS RSRERHRSRS RSRDRKGDRR DRDREREKEN 

       370        380        390        400        410        420 
ERGRRRDRDY DKERGNEREK ERERSRERSK EQRSRGEVEE KKHKEDKDDR RHRDDKRDSK 

       430        440        450        460        470        480 
KEKKHSRSRS RERKHRSRSR SRNAGKRSRS RSKEKSSKHK NESKEKSNKR SRSGSQGRTD 

       490        500        510        520        530        540 
SVEKSKKREH SPSKEKSRKR SRSKERSHKR DHSDSKDQSD KHDRRRSQSI EQESQEKQHK 


NKDETV 

« Hide

Isoform 2 [UniParc].

Checksum: BA711597136F2BBD
Show »

FASTA19021,061

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Lung, Muscle, Skin and Testis.
[4]"Immunomic analysis of human sarcoma."
Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J.
Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-546 (ISOFORM 1).
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527 AND SER-529, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527; SER-529 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[9]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-527; SER-529 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320; SER-473; SER-475; SER-481; SER-527 AND SER-529, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK001192 mRNA. Translation: BAA91546.1.
AL591719, AL160171 Genomic DNA. Translation: CAH72068.1.
AL591719, AL160171 Genomic DNA. Translation: CAH72069.1.
AL591719, AL160171 Genomic DNA. Translation: CAH72070.1. Sequence problems.
AL160171, AL591719 Genomic DNA. Translation: CAI12932.1.
AL160171, AL591719 Genomic DNA. Translation: CAI12933.1.
AL160171, AL591719 Genomic DNA. Translation: CAI12934.1. Sequence problems.
BC007757 mRNA. Translation: AAH07757.1. Different termination.
BC016296 mRNA. Translation: AAH16296.1. Different termination.
BC024275 mRNA. Translation: AAH24275.1. Different termination.
BC040127 mRNA. Translation: AAH40127.1. Different termination.
BC053838 mRNA. Translation: AAH53838.1.
BC107801 mRNA. Translation: AAI07802.1. Different termination.
BC130346 mRNA. Translation: AAI30347.1.
BC132963 mRNA. Translation: AAI32964.1.
AY211915 mRNA. Translation: AAO65168.1. Different initiation.
RefSeqNP_060531.2. NM_018061.2.
UniGeneHs.342307.

3D structure databases

ProteinModelPortalQ5VTL8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120428. 3 interactions.
IntActQ5VTL8. 7 interactions.
MINTMINT-4654815.

PTM databases

PhosphoSiteQ5VTL8.

Polymorphism databases

DMDM74746965.

Proteomic databases

PaxDbQ5VTL8.
PRIDEQ5VTL8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370022; ENSP00000359039; ENSG00000134186. [Q5VTL8-2]
ENST00000370025; ENSP00000359042; ENSG00000134186. [Q5VTL8-1]
GeneID55119.
KEGGhsa:55119.
UCSCuc001dvv.4. human. [Q5VTL8-1]

Organism-specific databases

CTD55119.
GeneCardsGC01P109234.
HGNCHGNC:25512. PRPF38B.
HPAHPA053255.
neXtProtNX_Q5VTL8.
PharmGKBPA142671126.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG266713.
HOVERGENHBG101033.
InParanoidQ5VTL8.
KOK12850.
OMAGPSFDRE.
OrthoDBEOG7XH6R2.
TreeFamTF313626.

Gene expression databases

BgeeQ5VTL8.
CleanExHS_PRPF38B.
GenevestigatorQ5VTL8.

Family and domain databases

InterProIPR005037. PRP38.
[Graphical view]
PANTHERPTHR23142. PTHR23142. 1 hit.
PfamPF03371. PRP38. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPRPF38B. human.
GenomeRNAi55119.
NextBio58764.
PROQ5VTL8.

Entry information

Entry namePR38B_HUMAN
AccessionPrimary (citable) accession number: Q5VTL8
Secondary accession number(s): Q05DD6 expand/collapse secondary AC list , Q32Q58, Q5VTL9, Q6PK39, Q7Z6E2, Q86WF3, Q8IWG9, Q9NW40
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM