Q5VTL8 (PR38B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor 38B

Alternative name(s):
Sarcoma antigen NY-SAR-27

Gene names

Name:

PRPF38B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be required for pre-mRNA splicing Potential.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the PRP38 family.
Sequence caution	The sequence AAO65168.1 differs from that shown. Reason: Erroneous initiation. The sequence CAH72070.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI12934.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5VTL8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5VTL8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     187-190: DLDV → FFTL
     191-546: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Pre-mRNA-splicing factor 38B		PRO_0000287235
Regions
	Coiled coil	292 – 321	30	Potential
	Compositional bias	253 – 451	199	Arg-rich
Amino acid modifications
	Modified residue	35	1	Phosphothreonine By similarity
	Modified residue	227	1	N6-acetyllysine Ref.8
	Modified residue	320	1	Phosphoserine Ref.10
	Modified residue	448	1	Phosphoserine Ref.9
	Modified residue	473	1	Phosphoserine Ref.10
	Modified residue	475	1	Phosphoserine Ref.10
	Modified residue	481	1	Phosphoserine Ref.10
	Modified residue	527	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.10
	Modified residue	529	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.10
	Modified residue	534	1	Phosphoserine Ref.7 Ref.9
Natural variations
	Alternative sequence	187 – 190	4	DLDV → FFTL in isoform 2.		VSP_025408
	Alternative sequence	191 – 546	356	Missing in isoform 2.		VSP_025409
Experimental info
	Sequence conflict	52	1	N → S in BAA91546. Ref.1
	Sequence conflict	294 – 295	2	EL → DR in AAH40127. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: A3082DCF491F88F8	FASTA	546	64,468
	10 20 30 40 50 60 MANNSPALTG NSQPQHQAAA AAAQQQQQCG GGGATKPAVS GKQGNVLPLW GNEKTMNLNP 70 80 90 100 110 120 MILTNILSSP YFKVQLYELK TYHEVVDEIY FKVTHVEPWE KGSRKTAGQT GMCGGVRGVG 130 140 150 160 170 180 TGGIVSTAFC LLYKLFTLKL TRKQVMGLIT HTDSPYIRAL GFMYIRYTQP PTDLWDWFES 190 200 210 220 230 240 FLDDEEDLDV KAGGGCVMTI GEMLRSFLTK LEWFSTLFPR IPVPVQKNID QQIKTRPRKI 250 260 270 280 290 300 KKDGKEGAEE IDRHVERRRS RSPRRSLSPR RSPRRSRSRS HHREGHGSSS FDRELEREKE 310 320 330 340 350 360 RQRLEREAKE REKERRRSRS IDRGLERRRS RSRERHRSRS RSRDRKGDRR DRDREREKEN 370 380 390 400 410 420 ERGRRRDRDY DKERGNEREK ERERSRERSK EQRSRGEVEE KKHKEDKDDR RHRDDKRDSK 430 440 450 460 470 480 KEKKHSRSRS RERKHRSRSR SRNAGKRSRS RSKEKSSKHK NESKEKSNKR SRSGSQGRTD 490 500 510 520 530 540 SVEKSKKREH SPSKEKSRKR SRSKERSHKR DHSDSKDQSD KHDRRRSQSI EQESQEKQHK NKDETV
	Isoform 2 [UniParc]. Checksum: BA711597136F2BBD	FASTA	190	21,061
	10 20 30 40 50 60 MANNSPALTG NSQPQHQAAA AAAQQQQQCG GGGATKPAVS GKQGNVLPLW GNEKTMNLNP 70 80 90 100 110 120 MILTNILSSP YFKVQLYELK TYHEVVDEIY FKVTHVEPWE KGSRKTAGQT GMCGGVRGVG 130 140 150 160 170 180 TGGIVSTAFC LLYKLFTLKL TRKQVMGLIT HTDSPYIRAL GFMYIRYTQP PTDLWDWFES 190 FLDDEEFFTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung, Muscle, Skin and Testis.
[4]	"Immunomic analysis of human sarcoma." Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-546 (ISOFORM 1).
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527 AND SER-529, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527; SER-529 AND SER-534, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-227, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-527; SER-529 AND SER-534, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320; SER-473; SER-475; SER-481; SER-527 AND SER-529, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001192 mRNA. Translation: BAA91546.1. AL591719, AL160171 Genomic DNA. Translation: CAH72068.1. AL591719, AL160171 Genomic DNA. Translation: CAH72069.1. AL591719, AL160171 Genomic DNA. Translation: CAH72070.1. Sequence problems. AL160171, AL591719 Genomic DNA. Translation: CAI12932.1. AL160171, AL591719 Genomic DNA. Translation: CAI12933.1. AL160171, AL591719 Genomic DNA. Translation: CAI12934.1. Sequence problems. BC007757 mRNA. Translation: AAH07757.1. Different termination. BC016296 mRNA. Translation: AAH16296.1. Different termination. BC024275 mRNA. Translation: AAH24275.1. Different termination. BC040127 mRNA. Translation: AAH40127.1. Different termination. BC053838 mRNA. Translation: AAH53838.1. BC107801 mRNA. Translation: AAI07802.1. Different termination. BC130346 mRNA. Translation: AAI30347.1. BC132963 mRNA. Translation: AAI32964.1. AY211915 mRNA. Translation: AAO65168.1. Different initiation.
IPI	IPI00018098. IPI00647851.
RefSeq	NP_060531.2. NM_018061.2.
UniGene	Hs.342307.
3D structure databases
ProteinModelPortal	Q5VTL8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5VTL8. 7 interactions.
MINT	MINT-4654815.
PTM databases
PhosphoSite	Q5VTL8.
Polymorphism databases
DMDM	74746965.
Proteomic databases
PaxDb	Q5VTL8.
PRIDE	Q5VTL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370021; ENSP00000359038; ENSG00000134186. ENST00000370022; ENSP00000359039; ENSG00000134186. ENST00000370025; ENSP00000359042; ENSG00000134186.
GeneID	55119.
KEGG	hsa:55119.
UCSC	uc001dvv.4. human.
Organism-specific databases
CTD	55119.
GeneCards	GC01P109234.
HGNC	HGNC:25512. PRPF38B.
HPA	HPA053255.
neXtProt	NX_Q5VTL8.
PharmGKB	PA142671126.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG266713.
HOVERGEN	HBG101033.
InParanoid	Q5VTL8.
KO	K12850.
OMA	SIEPESQ.
OrthoDB	EOG4NKBWJ.
Gene expression databases
Bgee	Q5VTL8.
CleanEx	HS_PRPF38B.
Genevestigator	Q5VTL8.
Family and domain databases
InterPro	IPR005037. PRP38. [Graphical view]
PANTHER	PTHR23142. PTHR23142. 1 hit.
Pfam	PF03371. PRP38. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PRPF38B. human.
GenomeRNAi	55119.
NextBio	58764.

Entry information

Entry name

PR38B_HUMAN

Accession

Primary (citable) accession number: Q5VTL8
Secondary accession number(s): Q05DD6 , Q32Q58, Q5VTL9, Q6PK39, Q7Z6E2, Q86WF3, Q8IWG9, Q9NW40

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 7, 2004
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.