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Protein

E3 ubiquitin-protein ligase RNF220

Gene

RNF220

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling (PubMed:25266658).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF220 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 220
Gene namesi
Name:RNF220
Synonyms:C1orf164
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25552. RNF220.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55182.
OpenTargetsiENSG00000187147.
PharmGKBiPA162401886.

Polymorphism and mutation databases

BioMutaiRNF220.
DMDMi74756788.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776571 – 566E3 ubiquitin-protein ligase RNF220Add BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei390PhosphoserineCombined sources1

Post-translational modificationi

Auto-ubiquitinated; leads to proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5VTB9.
MaxQBiQ5VTB9.
PaxDbiQ5VTB9.
PeptideAtlasiQ5VTB9.
PRIDEiQ5VTB9.

PTM databases

iPTMnetiQ5VTB9.
PhosphoSitePlusiQ5VTB9.

Expressioni

Gene expression databases

BgeeiENSG00000187147.
CleanExiHS_RNF220.
ExpressionAtlasiQ5VTB9. baseline and differential.
GenevisibleiQ5VTB9. HS.

Organism-specific databases

HPAiHPA027578.

Interactioni

Subunit structurei

Interacts with SIN3B (By similarity). Interacts with CTNNB1 (via Armadillo repeats 2-8) (PubMed:25266658). Interacts with USP7 (via MATH domain) (PubMed:25266658).By similarity1 Publication

Protein-protein interaction databases

BioGridi120481. 11 interactors.
IntActiQ5VTB9. 7 interactors.
MINTiMINT-4826954.
STRINGi9606.ENSP00000347548.

Structurei

3D structure databases

ProteinModelPortaliQ5VTB9.
SMRiQ5VTB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni514 – 522Required for targeting to the cytoplasmBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili485 – 513Sequence analysisAdd BLAST29

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEJC. Eukaryota.
ENOG4111JY9. LUCA.
GeneTreeiENSGT00390000016573.
HOGENOMiHOG000293278.
HOVERGENiHBG055526.
InParanoidiQ5VTB9.
OMAiSDRCKKK.
OrthoDBiEOG091G0PN3.
PhylomeDBiQ5VTB9.
TreeFamiTF324716.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031824. RNF220_mid.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF15926. RNF220. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VTB9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLHRAAFKM ENSSYLPNPL ASPALMVLAS TAEASRDASI PCQQPRPFGV
60 70 80 90 100
PVSVDKDVHI PFTNGSYTFA SMYHRQGGVP GTFANRDFPP SLLHLHPQFA
110 120 130 140 150
PPNLDCTPIS MLNHSGVGAF RPFASTEDRE SYQSAFTPAK RLKNCHDTES
160 170 180 190 200
PHLRFSDADG KEYDFGTQLP SSSPGSLKVD DTGKKIFAVS GLISDREASS
210 220 230 240 250
SPEDRNDRCK KKAAALFDSQ APICPICQVL LRPSELQEHM EQELEQLAQL
260 270 280 290 300
PSSKNSLLKD AMAPGTPKSL LLSASIKREG ESPTASPHSS ATDDLHHSDR
310 320 330 340 350
YQTFLRVRAN RQTRLNARIG KMKRRKQDEG QREGSCMAED DAVDIEHENN
360 370 380 390 400
NRFEEYEWCG QKRIRATTLL EGGFRGSGFI MCSGKENPDS DADLDVDGDD
410 420 430 440 450
TLEYGKPQYT EADVIPCTGE EPGEAKEREA LRGAVLNGGP PSTRITPEFS
460 470 480 490 500
KWASDEMPST SNGESSKQEA MQKTCKNSDI EKITEDSAVT TFEALKARVR
510 520 530 540 550
ELERQLSRGD RYKCLICMDS YSMPLTSIQC WHVHCEECWL RTLGAKKLCP
560
QCNTITAPGD LRRIYL
Length:566
Mass (Da):62,765
Last modified:December 7, 2004 - v1
Checksum:iAAE3C723032B062B
GO
Isoform 2 (identifier: Q5VTB9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.
     331-331: Q → QVCPLCNRPLAGSEQEMSRHVEHCLSK

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):39,570
Checksum:i9014236EFCF08F29
GO

Sequence cautioni

The sequence BAA91704 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84A → V in AAH98300 (PubMed:15489334).Curated1
Sequence conflicti277K → E in BAG51705 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554371 – 239Missing in isoform 2. 1 PublicationAdd BLAST239
Alternative sequenceiVSP_055438331Q → QVCPLCNRPLAGSEQEMSRH VEHCLSK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001459 mRNA. Translation: BAA91704.1. Different initiation.
AK056424 mRNA. Translation: BAG51705.1.
AK297228 mRNA. Translation: BAG59711.1.
AL596225, AL122004, AL359373 Genomic DNA. Translation: CAH72320.1.
AL359373, AL122004, AL596225 Genomic DNA. Translation: CAI16124.1.
AL122004, AL359373, AL596225 Genomic DNA. Translation: CAI22343.1.
BC034221 mRNA. Translation: AAH34221.1.
BC098266 mRNA. Translation: AAH98266.1.
BC098300 mRNA. Translation: AAH98300.1.
CCDSiCCDS510.1. [Q5VTB9-1]
RefSeqiNP_001306885.1. NM_001319956.1. [Q5VTB9-1]
NP_001306886.1. NM_001319957.1. [Q5VTB9-3]
NP_060620.2. NM_018150.3. [Q5VTB9-1]
XP_016857112.1. XM_017001623.1. [Q5VTB9-1]
XP_016857113.1. XM_017001624.1. [Q5VTB9-1]
XP_016857114.1. XM_017001625.1. [Q5VTB9-1]
UniGeneiHs.456557.

Genome annotation databases

EnsembliENST00000355387; ENSP00000347548; ENSG00000187147. [Q5VTB9-1]
ENST00000361799; ENSP00000354872; ENSG00000187147. [Q5VTB9-1]
ENST00000372247; ENSP00000361321; ENSG00000187147. [Q5VTB9-1]
GeneIDi55182.
KEGGihsa:55182.
UCSCiuc001clv.2. human. [Q5VTB9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001459 mRNA. Translation: BAA91704.1. Different initiation.
AK056424 mRNA. Translation: BAG51705.1.
AK297228 mRNA. Translation: BAG59711.1.
AL596225, AL122004, AL359373 Genomic DNA. Translation: CAH72320.1.
AL359373, AL122004, AL596225 Genomic DNA. Translation: CAI16124.1.
AL122004, AL359373, AL596225 Genomic DNA. Translation: CAI22343.1.
BC034221 mRNA. Translation: AAH34221.1.
BC098266 mRNA. Translation: AAH98266.1.
BC098300 mRNA. Translation: AAH98300.1.
CCDSiCCDS510.1. [Q5VTB9-1]
RefSeqiNP_001306885.1. NM_001319956.1. [Q5VTB9-1]
NP_001306886.1. NM_001319957.1. [Q5VTB9-3]
NP_060620.2. NM_018150.3. [Q5VTB9-1]
XP_016857112.1. XM_017001623.1. [Q5VTB9-1]
XP_016857113.1. XM_017001624.1. [Q5VTB9-1]
XP_016857114.1. XM_017001625.1. [Q5VTB9-1]
UniGeneiHs.456557.

3D structure databases

ProteinModelPortaliQ5VTB9.
SMRiQ5VTB9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120481. 11 interactors.
IntActiQ5VTB9. 7 interactors.
MINTiMINT-4826954.
STRINGi9606.ENSP00000347548.

PTM databases

iPTMnetiQ5VTB9.
PhosphoSitePlusiQ5VTB9.

Polymorphism and mutation databases

BioMutaiRNF220.
DMDMi74756788.

Proteomic databases

EPDiQ5VTB9.
MaxQBiQ5VTB9.
PaxDbiQ5VTB9.
PeptideAtlasiQ5VTB9.
PRIDEiQ5VTB9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355387; ENSP00000347548; ENSG00000187147. [Q5VTB9-1]
ENST00000361799; ENSP00000354872; ENSG00000187147. [Q5VTB9-1]
ENST00000372247; ENSP00000361321; ENSG00000187147. [Q5VTB9-1]
GeneIDi55182.
KEGGihsa:55182.
UCSCiuc001clv.2. human. [Q5VTB9-1]

Organism-specific databases

CTDi55182.
DisGeNETi55182.
GeneCardsiRNF220.
H-InvDBHIX0000517.
HGNCiHGNC:25552. RNF220.
HPAiHPA027578.
MIMi616136. gene.
neXtProtiNX_Q5VTB9.
OpenTargetsiENSG00000187147.
PharmGKBiPA162401886.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEJC. Eukaryota.
ENOG4111JY9. LUCA.
GeneTreeiENSGT00390000016573.
HOGENOMiHOG000293278.
HOVERGENiHBG055526.
InParanoidiQ5VTB9.
OMAiSDRCKKK.
OrthoDBiEOG091G0PN3.
PhylomeDBiQ5VTB9.
TreeFamiTF324716.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiRNF220. human.
GeneWikiiRNF220.
GenomeRNAii55182.
PROiQ5VTB9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187147.
CleanExiHS_RNF220.
ExpressionAtlasiQ5VTB9. baseline and differential.
GenevisibleiQ5VTB9. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031824. RNF220_mid.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF15926. RNF220. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRN220_HUMAN
AccessioniPrimary (citable) accession number: Q5VTB9
Secondary accession number(s): B3KPJ3
, B4DLZ9, E9PCS1, Q4KMX2, Q9NVP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.