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Q5VT66 (MARC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial amidoxime-reducing component 1

Short name=mARC1
EC=1.-.-.-
Alternative name(s):
Molybdenum cofactor sulfurase C-terminal domain-containing protein 1
Short name=MOSC domain-containing protein 1
Short name=Moco sulfurase C-terminal domain-containing protein 1
Gene names
Name:MARC1
Synonyms:MOSC1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

As a component of an N-hydroxylated prodrug-converting complex required to reduce N-hydroxylated prodrugs, such as benzamidoxime. Also able to reduce N(omega)-hydroxy-L-arginine (NOHA) and N(omega)-hydroxy-N(delta)-methyl-L-arginine (NHAM) into L-arginine and N(delta)-methyl-L-arginine, respectively. Ref.6

Cofactor

Molybdenum. Ref.6 Ref.10

Subunit structure

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase and MARC1.

Subcellular location

Mitochondrion outer membrane; Single-pass type II membrane protein. Note: Mitochondrial import is mediated by AA 1-40 and requires ATP. Ref.9

Sequence similarities

Contains 1 MOSC domain.

Biophysicochemical properties

Kinetic parameters:

KM=180 µM for benzamidoxime Ref.8 Ref.10

KM=86 µM for NOHA

KM=272 µM for NHAM

Vmax=34 nmol/min/mg enzyme toward benzamidoxime (Ref.8)

Vmax=105 nmol/min/mg enzyme toward benzamidoxime (Ref.10, at pH 6.0 and 37 degrees Celsius)

Vmax=55 nmol/min/mg enzyme toward NOHA

Vmax=43 nmol/min/mg enzyme toward NHAM

Sequence caution

The sequence BAB15333.1 differs from that shown. Reason: Frameshift at position 139.

The sequence CAH72118.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5VT66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5VT66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     251-251: E → EVTLCPFGSFLGFDFFFK
Note: No experimental confirmation available.
Isoform 3 (identifier: Q5VT66-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.
     251-251: E → EVTLCPFGSFLGFDFFFK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Mitochondrial amidoxime-reducing component 1
PRO_0000273335

Regions

Topological domain1 – 2020Mitochondrial matrix Ref.9
Transmembrane21 – 4020Helical; Signal-anchor for type II membrane protein; Potential
Topological domain41 – 337297Cytoplasmic Ref.9
Domain187 – 335149MOSC

Natural variations

Alternative sequence1 – 102102Missing in isoform 3.
VSP_022511
Alternative sequence2511E → EVTLCPFGSFLGFDFFFK in isoform 2 and isoform 3.
VSP_022512
Natural variant151L → H. Ref.2
VAR_062273
Natural variant961V → L Common polymorphism in Caucasian population; no effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.2 Ref.10
Corresponds to variant rs12023067 [ dbSNP | Ensembl ].
VAR_056941
Natural variant1651T → A No effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.1 Ref.2 Ref.4 Ref.5 Ref.10
Corresponds to variant rs2642438 [ dbSNP | Ensembl ].
VAR_030129
Natural variant1871M → K No effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.5 Ref.10
Corresponds to variant rs17850677 [ dbSNP | Ensembl ].
VAR_030130
Natural variant2461C → S No effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.2 Ref.10
Corresponds to variant rs3738178 [ dbSNP | Ensembl ].
VAR_030131
Natural variant2471D → H No effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.2 Ref.10
Corresponds to variant rs72470601 [ dbSNP | Ensembl ].
VAR_062274
Natural variant2681M → I No effect on binding of the molybdenum cofactor; no significant effect on catalytic efficiency toward benzamidoxime; no significant effect on affinity for benzamidoxime. Ref.10
Corresponds to variant rs2642419 [ dbSNP | Ensembl ].
VAR_030132

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: F983AB08F3D646C4

FASTA33737,499
        10         20         30         40         50         60 
MGAAGSSALA RFVLLAQSRP GWLGVAALGL TAVALGAVAW RRAWPTRRRR LLQQVGTVAQ 

        70         80         90        100        110        120 
LWIYPVKSCK GVPVSEAECT AMGLRSGNLR DRFWLVINQE GNMVTARQEP RLVLISLTCD 

       130        140        150        160        170        180 
GDTLTLSAAY TKDLLLPIKT PTTNAVHKCR VHGLEIEGRD CGEATAQWIT SFLKSQPYRL 

       190        200        210        220        230        240 
VHFEPHMRPR RPHQIADLFR PKDQIAYSDT SPFLILSEAS LADLNSRLEK KVKATNFRPN 

       250        260        270        280        290        300 
IVISGCDVYA EDSWDELLIG DVELKRVMAC SRCILTTVDP DTGVMSRKEP LETLKSYRQC 

       310        320        330 
DPSERKLYGK SPLFGQYFVL ENPGTIKVGD PVYLLGQ 

« Hide

Isoform 2 [UniParc].

Checksum: CC368AD4B543AD51
Show »

FASTA35439,454
Isoform 3 [UniParc].

Checksum: 0FEF88317AF6C9D2
Show »

FASTA25228,439

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT ALA-165.
Tissue: Adrenal gland and Kidney.
[2]NIEHS SNPs program
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-15; LEU-96; ALA-165; SER-246 AND HIS-247.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-165.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ALA-165 AND LYS-187.
Tissue: Eye.
[6]"The fourth molybdenum containing enzyme mARC: cloning and involvement in the activation of N-hydroxylated prodrugs."
Gruenewald S., Wahl B., Bittner F., Hungeling H., Kanzow S., Kotthaus J., Schwering U., Mendel R.R., Clement B.
J. Med. Chem. 51:8173-8177(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Reduction of N(omega)-hydroxy-L-arginine by the mitochondrial amidoxime reducing component (mARC)."
Kotthaus J., Wahl B., Havemeyer A., Kotthaus J., Schade D., Garbe-Schonberg D., Mendel R., Bittner F., Clement B.
Biochem. J. 433:383-391(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[9]"The mitochondrial amidoxime-reducing component (mARC1) is a novel signal-anchored protein of the outer mitochondrial membrane."
Klein J.M., Busch J.D., Potting C., Baker M.J., Langer T., Schwarz G.
J. Biol. Chem. 287:42795-42803(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
[10]"Functional characterization of protein variants encoded by non-synonymous SNPs in MARC1 and MARC2 in healthy Caucasians."
Ott G., Reichmann D., Boerger C., Cascorbi I., Bittner F., Mendel R.R., Kunze T., Clement B., Havemeyer A.
Drug Metab. Dispos. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS ALA-165; LYS-187; SER-246 AND HIS-247, CHARACTERIZATION OF VARIANTS LEU-96; ALA-165; LYS-187; SER-246; HIS-247 AND ILE-268, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK026043 mRNA. Translation: BAB15333.1. Frameshift.
AK094105 mRNA. Translation: BAC04286.1.
AK290812 mRNA. Translation: BAF83501.1.
EU563849 Genomic DNA. Translation: ACB21046.1.
AL606726, AL445423 Genomic DNA. Translation: CAH71881.1.
AL445423 Genomic DNA. Translation: CAH72116.1.
AL445423 Genomic DNA. Translation: CAH72118.1. Sequence problems.
AL445423, AL606726 Genomic DNA. Translation: CAH72119.1.
CH471100 Genomic DNA. Translation: EAW93291.1.
BC010619 mRNA. Translation: AAH10619.1.
RefSeqNP_073583.3. NM_022746.3.
UniGeneHs.497816.

3D structure databases

ProteinModelPortalQ5VT66.
SMRQ5VT66. Positions 58-188.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122271. 13 interactions.
STRING9606.ENSP00000355877.

PTM databases

PhosphoSiteQ5VT66.

Polymorphism databases

DMDM74746896.

Proteomic databases

PaxDbQ5VT66.
PRIDEQ5VT66.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000366910; ENSP00000355877; ENSG00000186205. [Q5VT66-1]
GeneID64757.
KEGGhsa:64757.
UCSCuc001hms.3. human. [Q5VT66-1]
uc001hmt.3. human. [Q5VT66-2]

Organism-specific databases

CTD64757.
GeneCardsGC01P220961.
H-InvDBHIX0001608.
HGNCHGNC:26189. MARC1.
HPAHPA028702.
MIM614126. gene.
neXtProtNX_Q5VT66.
PharmGKBPA142671344.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG3217.
HOVERGENHBG081982.
InParanoidQ5VT66.
OMAKSYRQCD.
OrthoDBEOG7WT41P.
PhylomeDBQ5VT66.
TreeFamTF316807.

Gene expression databases

BgeeQ5VT66.
CleanExHS_MOSC1.
GenevestigatorQ5VT66.

Family and domain databases

InterProIPR005302. MoCF_Sase_C.
IPR005303. MOSC_N.
IPR011037. Pyrv_Knase-like_insert_dom.
[Graphical view]
PfamPF03473. MOSC. 1 hit.
PF03476. MOSC_N. 1 hit.
[Graphical view]
SUPFAMSSF50800. SSF50800. 2 hits.
PROSITEPS51340. MOSC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi64757.
NextBio66731.
PROQ5VT66.
SOURCESearch...

Entry information

Entry nameMARC1_HUMAN
AccessionPrimary (citable) accession number: Q5VT66
Secondary accession number(s): A8K447 expand/collapse secondary AC list , B2D078, Q5VVS9, Q5VVT0, Q5VVT1, Q8N9P5, Q96FN8, Q9H6C7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM