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Protein

G kinase-anchoring protein 1

Gene

GKAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
G kinase-anchoring protein 1
Alternative name(s):
cGMP-dependent protein kinase-anchoring protein of 42 kDa
Gene namesi
Name:GKAP1
Synonyms:GKAP42
ORF Names:FKSG21
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:17496. GKAP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134880503.

Polymorphism and mutation databases

BioMutaiGKAP1.
DMDMi166224631.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366G kinase-anchoring protein 1PRO_0000315654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphoserine2 Publications
Modified residuei106 – 1061Phosphoserine; by PKGBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5VSY0.
PaxDbiQ5VSY0.
PRIDEiQ5VSY0.

PTM databases

PhosphoSiteiQ5VSY0.

Expressioni

Gene expression databases

BgeeiQ5VSY0.
CleanExiHS_GKAP1.
ExpressionAtlasiQ5VSY0. baseline and differential.
GenevisibleiQ5VSY0. HS.

Organism-specific databases

HPAiHPA035117.
HPA035118.

Interactioni

Subunit structurei

Interacts with PRKG1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DYRB0YJ763EBI-743722,EBI-10175413
HDDC3Q8N4P33EBI-743722,EBI-750003
MFAP1P550813EBI-743722,EBI-1048159

Protein-protein interaction databases

BioGridi123231. 12 interactions.
DIPiDIP-47324N.
IntActiQ5VSY0. 11 interactions.
MINTiMINT-1455843.
STRINGi9606.ENSP00000365550.

Structurei

3D structure databases

ProteinModelPortaliQ5VSY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili47 – 7731Sequence AnalysisAdd
BLAST
Coiled coili128 – 16033Sequence AnalysisAdd
BLAST
Coiled coili243 – 353111Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the GKAP1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG39546.
GeneTreeiENSGT00390000008742.
HOGENOMiHOG000088604.
HOVERGENiHBG107948.
InParanoidiQ5VSY0.
OMAiQQGEMKD.
OrthoDBiEOG7GJ6D6.
PhylomeDBiQ5VSY0.
TreeFamiTF328459.

Family and domain databases

InterProiIPR026109. GKAP1.
[Graphical view]
PANTHERiPTHR14899. PTHR14899. 1 hit.
PRINTSiPR02083. GKINASEAP1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5VSY0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASAVLSSVP TTASRFALLQ VDSGSGSDSE PGKGKGRNTG KSQTLGSKST
60 70 80 90 100
TNEKKREKRR KKKEQQQSEA NELRNLAFKK IPQKSSHAVC NAQHDLPLSN
110 120 130 140 150
PVQKDSREEN WQEWRQRDEQ LTSEMFEADL EKALLLSKLE YEEHKKEYED
160 170 180 190 200
AENTSTQSKV MNKKDKRKNH QGKDRPLTVS LKDFHSEDHI SKKTEELSSS
210 220 230 240 250
QTLSHDGGFF NRLEDDVHKI LIREKRREQL TEYNGTDNCT AHEHNQEVVL
260 270 280 290 300
KDGRIERLKL ELERKDAEIQ KLKNVITQWE AKYKEVKARN AQLLKMLQEG
310 320 330 340 350
EMKDKAEILL QVDESQSIKN ELTIQVTSLH AALEQERSKV KVLQAELAKY
360
QGGRKGKRNS ESDQCR
Length:366
Mass (Da):42,078
Last modified:January 15, 2008 - v2
Checksum:i973D16361756691B
GO
Isoform 2 (identifier: Q5VSY0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     196-246: Missing.

Show »
Length:315
Mass (Da):36,126
Checksum:i90AEB71AF18F1CEE
GO

Sequence cautioni

The sequence BAB71712.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAH70734.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI16011.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAW62665.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3251Q → R in AAG40320 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei196 – 24651Missing in isoform 2. 1 PublicationVSP_030596Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033131 mRNA. Translation: BAB40454.1.
AB033132 mRNA. Translation: BAB40455.1.
AF319476 mRNA. Translation: AAG40320.1.
AL662787, AL354733 Genomic DNA. Translation: CAH70734.1. Sequence problems.
AL662787, AL354733 Genomic DNA. Translation: CAH70735.1.
AL354733, AL662787 Genomic DNA. Translation: CAI16010.1.
AL354733, AL662787 Genomic DNA. Translation: CAI16011.1. Sequence problems.
CH471089 Genomic DNA. Translation: EAW62665.1. Sequence problems.
CH471089 Genomic DNA. Translation: EAW62666.1.
BC014476 mRNA. Translation: AAH14476.1.
AK058198 mRNA. Translation: BAB71712.1. Different initiation.
CCDSiCCDS35049.1. [Q5VSY0-1]
CCDS47988.1. [Q5VSY0-2]
RefSeqiNP_001129425.1. NM_001135953.1. [Q5VSY0-2]
NP_079487.2. NM_025211.3. [Q5VSY0-1]
XP_005252298.1. XM_005252241.1. [Q5VSY0-1]
XP_011517360.1. XM_011519058.1. [Q5VSY0-1]
UniGeneiHs.522255.

Genome annotation databases

EnsembliENST00000376365; ENSP00000365544; ENSG00000165113. [Q5VSY0-2]
ENST00000376371; ENSP00000365550; ENSG00000165113.
GeneIDi80318.
KEGGihsa:80318.
UCSCiuc004amy.3. human. [Q5VSY0-1]
uc004amz.3. human. [Q5VSY0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033131 mRNA. Translation: BAB40454.1.
AB033132 mRNA. Translation: BAB40455.1.
AF319476 mRNA. Translation: AAG40320.1.
AL662787, AL354733 Genomic DNA. Translation: CAH70734.1. Sequence problems.
AL662787, AL354733 Genomic DNA. Translation: CAH70735.1.
AL354733, AL662787 Genomic DNA. Translation: CAI16010.1.
AL354733, AL662787 Genomic DNA. Translation: CAI16011.1. Sequence problems.
CH471089 Genomic DNA. Translation: EAW62665.1. Sequence problems.
CH471089 Genomic DNA. Translation: EAW62666.1.
BC014476 mRNA. Translation: AAH14476.1.
AK058198 mRNA. Translation: BAB71712.1. Different initiation.
CCDSiCCDS35049.1. [Q5VSY0-1]
CCDS47988.1. [Q5VSY0-2]
RefSeqiNP_001129425.1. NM_001135953.1. [Q5VSY0-2]
NP_079487.2. NM_025211.3. [Q5VSY0-1]
XP_005252298.1. XM_005252241.1. [Q5VSY0-1]
XP_011517360.1. XM_011519058.1. [Q5VSY0-1]
UniGeneiHs.522255.

3D structure databases

ProteinModelPortaliQ5VSY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123231. 12 interactions.
DIPiDIP-47324N.
IntActiQ5VSY0. 11 interactions.
MINTiMINT-1455843.
STRINGi9606.ENSP00000365550.

PTM databases

PhosphoSiteiQ5VSY0.

Polymorphism and mutation databases

BioMutaiGKAP1.
DMDMi166224631.

Proteomic databases

MaxQBiQ5VSY0.
PaxDbiQ5VSY0.
PRIDEiQ5VSY0.

Protocols and materials databases

DNASUi80318.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376365; ENSP00000365544; ENSG00000165113. [Q5VSY0-2]
ENST00000376371; ENSP00000365550; ENSG00000165113.
GeneIDi80318.
KEGGihsa:80318.
UCSCiuc004amy.3. human. [Q5VSY0-1]
uc004amz.3. human. [Q5VSY0-2]

Organism-specific databases

CTDi80318.
GeneCardsiGC09M086354.
HGNCiHGNC:17496. GKAP1.
HPAiHPA035117.
HPA035118.
MIMi611356. gene.
neXtProtiNX_Q5VSY0.
PharmGKBiPA134880503.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39546.
GeneTreeiENSGT00390000008742.
HOGENOMiHOG000088604.
HOVERGENiHBG107948.
InParanoidiQ5VSY0.
OMAiQQGEMKD.
OrthoDBiEOG7GJ6D6.
PhylomeDBiQ5VSY0.
TreeFamiTF328459.

Miscellaneous databases

ChiTaRSiGKAP1. human.
GenomeRNAii80318.
NextBioi70827.
PROiQ5VSY0.
SOURCEiSearch...

Gene expression databases

BgeeiQ5VSY0.
CleanExiHS_GKAP1.
ExpressionAtlasiQ5VSY0. baseline and differential.
GenevisibleiQ5VSY0. HS.

Family and domain databases

InterProiIPR026109. GKAP1.
[Graphical view]
PANTHERiPTHR14899. PTHR14899. 1 hit.
PRINTSiPR02083. GKINASEAP1.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha."
    Yuasa K., Omori K., Yanaka N.
    J. Biol. Chem. 275:4897-4905(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "Cloning of FKSG21, a novel gene located on human chromosome 9."
    Wang Y.-G., Gong L.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-366 (ISOFORM 1).
    Tissue: Testis.
  7. "Delineation of the minimal commonly deleted segment and identification of candidate tumor-suppressor genes in del(9q) acute myeloid leukemia."
    Sweetser D.A., Peniket A.J., Haaland C., Blomberg A.A., Zhang Y., Zaidi S.T., Dayyani F., Zhao Z., Heerema N.A., Boultwood J., Dewald G.W., Paietta E., Slovak M.L., Willman C.L., Wainscoat J.S., Bernstein I.D., Daly S.B.
    Genes Chromosomes Cancer 44:279-291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiGKAP1_HUMAN
AccessioniPrimary (citable) accession number: Q5VSY0
Secondary accession number(s): Q96LI0
, Q9BYI1, Q9BYI2, Q9H225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: July 22, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.