Q5VRH4 (HGD_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	HGO
Ordered Locus Names:	Os06g0103300, LOC_Os06g01360
ORF Names:	OSJNBa0075G19.26

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Homogentisate 1,2-dioxygenase		PRO_0000247478
Sites
	Metal binding	356	1	Iron By similarity
	Metal binding	362	1	Iron By similarity
	Metal binding	392	1	Iron By similarity
Experimental info
	Sequence conflict	421	1	M → V Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5VRH4 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 9C2148F4ABB079E4	FASTA	470	51,771
	10 20 30 40 50 60 MAMATATPAA QNEQQEKGGL EYVYLSGLGN SLSSEAVAGT LPRGQNSPLV CPLGLYAEQL 70 80 90 100 110 120 SGTPFTAPRA RNLRTWLYRI KPSVTHEPFH PRRPAHPRLI GDFDRTTTDT VATPTQLRWR 130 140 150 160 170 180 PADVPPHHPP LDFIDGLYTV CGAGSSFLRH GYAIHMYAAN KSMDGCAFCN ADGDFLIVPQ 190 200 210 220 230 240 QGKLLITTEC GKLLVPPGEI VVIPQGFRFA VDLPDGPSRG YVSEIFGTHF QLPDLGPIGA 250 260 270 280 290 300 NGLASARDFL SPTAWFEQVH RPGYTIVQKY GGELFTATQD FSPFNVVAWH GNYVPYKYDL 310 320 330 340 350 360 SKFCPFNTVL FDHADPSVNT VLTAPTDKPG VALLDFVIFP PRWLVAENTF RPPYYHRNCM 370 380 390 400 410 420 SEFMGLIYGI YEAKADGFLP GGASLHSCMT PHGPDTKTYE ATISRPDANE PSRLSGTLAF 430 440 450 460 470 MFESALIPRV CQWALDSPSR DLDYYQCWIG LKSHFSHDNG GATSEEPCRK

References

[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002842 Genomic DNA. Translation: BAD67951.1. AK065189 mRNA. No translation available.
RefSeq	NP_001056540.1. NM_001063075.1.
UniGene	Os.11622.
3D structure databases
ProteinModelPortal	Q5VRH4.
ModBase	Search...
Proteomic databases
PRIDE	Q5VRH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g01360.1; LOC_Os06g01360.1; LOC_Os06g01360.
GeneID	4339844.
KEGG	dosa:Os06t0103300-01. osa:4339844.
Organism-specific databases
Gramene	Q5VRH4.
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PLN02658.
Enzyme and pathway databases
UniPathway	UPA00139; UER00339.
Gene expression databases
ArrayExpress	Q5VRH4.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
InterPro	IPR005708. Homogentis_dOase. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_ORYSJ

Accession

Primary (citable) accession number: Q5VRH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program