Reviewed, UniProtKB/Swiss-Prot Q5VRH4 (HGD_ORYSJ)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisic acid oxidase
    Homogentisate oxygenase
    Homogentisicase

Gene names

Name:	HGO
Ordered Locus Names:	Os06g0103300, LOC_Os06g01360
ORF Names:	OSJNBa0075G19.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Homogentisate 1,2-dioxygenase		PRO_0000247478
Sites
	Metal binding	356	1	Iron By similarity
	Metal binding	362	1	Iron By similarity
	Metal binding	392	1	Iron By similarity
Experimental info
	Sequence conflict	421	1	M → V Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5VRH4-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 9C2148F4ABB079E4	FASTA	470	51,771
	10 20 30 40 50 60 MAMATATPAA QNEQQEKGGL EYVYLSGLGN SLSSEAVAGT LPRGQNSPLV CPLGLYAEQL 70 80 90 100 110 120 SGTPFTAPRA RNLRTWLYRI KPSVTHEPFH PRRPAHPRLI GDFDRTTTDT VATPTQLRWR 130 140 150 160 170 180 PADVPPHHPP LDFIDGLYTV CGAGSSFLRH GYAIHMYAAN KSMDGCAFCN ADGDFLIVPQ 190 200 210 220 230 240 QGKLLITTEC GKLLVPPGEI VVIPQGFRFA VDLPDGPSRG YVSEIFGTHF QLPDLGPIGA 250 260 270 280 290 300 NGLASARDFL SPTAWFEQVH RPGYTIVQKY GGELFTATQD FSPFNVVAWH GNYVPYKYDL 310 320 330 340 350 360 SKFCPFNTVL FDHADPSVNT VLTAPTDKPG VALLDFVIFP PRWLVAENTF RPPYYHRNCM 370 380 390 400 410 420 SEFMGLIYGI YEAKADGFLP GGASLHSCMT PHGPDTKTYE ATISRPDANE PSRLSGTLAF 430 440 450 460 470 MFESALIPRV CQWALDSPSR DLDYYQCWIG LKSHFSHDNG GATSEEPCRK

References

[1]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP002842 Genomic DNA. Translation: BAD67951.1. AK065189 mRNA. No translation available.
RefSeq	NP_001056540.1.
UniGene	Os.11622
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4339844.
KEGG	osa:4339844.
Organism-specific databases
Gramene	Q5VRH4.
Family and domain databases
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_ORYSJ

Accession

Primary (citable) accession number: Q5VRH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)