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Q5VQG4 (RFS_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Galactinol--sucrose galactosyltransferase
EC=2.4.1.82
Alternative name(s):
Raffinose synthase
Gene names
Name:RFS
Ordered Locus Names:Os01g0170000, LOC_Os01g07530
ORF Names:OJ1276_B06.38, OsJ_00538, P0583G08.2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length783 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors. Able to utilize sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose as acceptors. May also act as a glycoside hydrolase. Ref.1

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose. Ref.1

Enzyme regulation

Inhibited by Ag2+, Hg2+, Zn2+, p-chloromercuribenzoate (pCMB) and 1-deoxygalactonojirimycin.

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Biophysicochemical properties

Kinetic parameters:

Vmax=25.6 nmol/sec/mg enzyme toward raffinose for the raffinose synthesis activity Ref.1

Vmax=6.8 nmol/sec/mg enzyme toward p-nitrophenyl-alpha-D-galactoside for the hydrolysis activity

Vmax=8.5 nmol/sec/mg enzyme toward galactinol for the hydrolysis activity

Vmax=5.7 nmol/sec/mg enzyme toward raffinose for the hydrolysis activity

pH dependence:

Optimum pH is 7.0.

Temperature dependence:

Optimum temperature is 45 degrees Celsius.

Sequence caution

The sequence BAF04051.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiongalactinol-sucrose galactosyltransferase activity

Inferred from direct assay Ref.1. Source: Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Probable
Chain2 – 783782Galactinol--sucrose galactosyltransferase
PRO_0000389255

Regions

Compositional bias66 – 694Poly-Ala
Compositional bias273 – 2764Poly-Asp
Compositional bias642 – 6465Poly-Gly

Sequences

Sequence LengthMass (Da)Tools
Q5VQG4 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 59BB443A5965DC1D

FASTA78385,533
        10         20         30         40         50         60 
MAPNLSKAKD DLIGDVVAVD GLIKPPRFTL KGKDLAVDGH PFLLDVPANI RLTPASTLVP 

        70         80         90        100        110        120 
NSDVPAAAAG SFLGFDAPAA KDRHVVPIGK LRDTRFMSIF RFKVWWTTHW VGTNGRDVEN 

       130        140        150        160        170        180 
ETQMMILDQS GTKSSPTGPR PYVLLLPIVE GPFRACLESG KAEDYVHMVL ESGSSTVRGS 

       190        200        210        220        230        240 
VFRSAVYLHA GDDPFDLVKD AMRVVRAHLG TFRLMEEKTP PPIVDKFGWC TWDAFYLKVH 

       250        260        270        280        290        300 
PEGVWEGVRR LADGGCPPGL VLIDDGWQSI CHDDDDLGSG AEGMNRTSAG EQMPCRLIKF 

       310        320        330        340        350        360 
QENYKFREYK GGMGGFVREM KAAFPTVEQV YVWHALCGYW GGLRPGAPGL PPAKVVAPRL 

       370        380        390        400        410        420 
SPGLQRTMED LAVDKIVNNG VGLVDPRRAR ELYEGLHSHL QASGIDGVKV DVIHLLEMVC 

       430        440        450        460        470        480 
EEYGGRVELA KAYFAGLTES VRRHFNGNGV IASMEHCNDF MLLGTEAVAL GRVGDDFWCT 

       490        500        510        520        530        540 
DPSGDPDGTF WLQGCHMVHC AYNSLWMGAF IHPDWDMFQS THPCAAFHAA SRAVSGGPVY 

       550        560        570        580        590        600 
VSDAVGCHDF DLLRRLALPD GTILRCERYA LPTRDCLFAD PLHDGKTMLK IWNVNKFSGV 

       610        620        630        640        650        660 
LGAFNCQGGG WSREARRNMC AAGFSVPVTA RASPADVEWS HGGGGGDRFA VYFVEARKLQ 

       670        680        690        700        710        720 
LLRRDESVEL TLEPFTYELL VVAPVRAIVS PELGIGFAPI GLANMLNAGG AVQGFEAARK 

       730        740        750        760        770        780 
DGDVAAEVAV KGAGEMVAYS SARPRLCKVN GQDAEFKYED GIVTVDVPWT GSSKKLSRVE 


YFY

« Hide

References

« Hide 'large scale' references
[1]"Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare)."
Li S., Li T., Kim W.-D., Kitaoka M., Yoshida S., Nakajima M., Kobayashi H.
Biotechnol. Lett. 29:635-640(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[2]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP003282 Genomic DNA. Translation: BAD68247.1.
AP003339 Genomic DNA. Translation: BAD68321.1.
AP008207 Genomic DNA. Translation: BAF04051.1. Sequence problems.
CM000138 Genomic DNA. Translation: EAZ10704.1.
RefSeqNP_001042137.1. NM_001048672.1.
UniGeneOs.61038.

3D structure databases

ProteinModelPortalQ5VQG4.
ModBaseSearch...

Proteomic databases

PaxDbQ5VQG4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4325200.
KEGGdosa:Os01t0170000-01.
osa:4325200.

Organism-specific databases

GrameneQ5VQG4.

Phylogenomic databases

eggNOGNOG287560.
KOK06617.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Raffinose_synthase.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRFS_ORYSJ
AccessionPrimary (citable) accession number: Q5VQG4
Secondary accession number(s): Q0JQC7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: December 7, 2004
Last modified: April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents