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Q5VQG4

- RFS_ORYSJ

UniProt

Q5VQG4 - RFS_ORYSJ

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Protein

Galactinol--sucrose galactosyltransferase

Gene

RFS

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors. Able to utilize sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose as acceptors. May also act as a glycoside hydrolase.1 Publication

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.1 Publication

Enzyme regulationi

Inhibited by Ag2+, Hg2+, Zn2+, p-chloromercuribenzoate (pCMB) and 1-deoxygalactonojirimycin.

Kineticsi

    Vmax=25.6 nmol/sec/mg enzyme toward raffinose for the raffinose synthesis activity1 Publication

    Vmax=6.8 nmol/sec/mg enzyme toward p-nitrophenyl-alpha-D-galactoside for the hydrolysis activity1 Publication

    Vmax=8.5 nmol/sec/mg enzyme toward galactinol for the hydrolysis activity1 Publication

    Vmax=5.7 nmol/sec/mg enzyme toward raffinose for the hydrolysis activity1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. galactinol-sucrose galactosyltransferase activity Source: Gramene

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. response to abscisic acid Source: EnsemblPlants/Gramene
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactinol--sucrose galactosyltransferase (EC:2.4.1.82)
    Alternative name(s):
    Raffinose synthase
    Gene namesi
    Name:RFS
    Ordered Locus Names:Os01g0170000, LOC_Os01g07530
    ORF Names:OJ1276_B06.38, OsJ_00538, P0583G08.2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ5VQG4.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 783782Galactinol--sucrose galactosyltransferasePRO_0000389255Add
    BLAST

    Proteomic databases

    PaxDbiQ5VQG4.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os01g07530.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5VQG4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi66 – 694Poly-Ala
    Compositional biasi273 – 2764Poly-Asp
    Compositional biasi642 – 6465Poly-Gly

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG287560.
    InParanoidiQ5VQG4.
    KOiK06617.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5VQG4-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    MAPNLSKAKD DLIGDVVAVD GLIKPPRFTL KGKDLAVDGH PFLLDVPANI
    60 70 80 90 100
    RLTPASTLVP NSDVPAAAAG SFLGFDAPAA KDRHVVPIGK LRDTRFMSIF
    110 120 130 140 150
    RFKVWWTTHW VGTNGRDVEN ETQMMILDQS GTKSSPTGPR PYVLLLPIVE
    160 170 180 190 200
    GPFRACLESG KAEDYVHMVL ESGSSTVRGS VFRSAVYLHA GDDPFDLVKD
    210 220 230 240 250
    AMRVVRAHLG TFRLMEEKTP PPIVDKFGWC TWDAFYLKVH PEGVWEGVRR
    260 270 280 290 300
    LADGGCPPGL VLIDDGWQSI CHDDDDLGSG AEGMNRTSAG EQMPCRLIKF
    310 320 330 340 350
    QENYKFREYK GGMGGFVREM KAAFPTVEQV YVWHALCGYW GGLRPGAPGL
    360 370 380 390 400
    PPAKVVAPRL SPGLQRTMED LAVDKIVNNG VGLVDPRRAR ELYEGLHSHL
    410 420 430 440 450
    QASGIDGVKV DVIHLLEMVC EEYGGRVELA KAYFAGLTES VRRHFNGNGV
    460 470 480 490 500
    IASMEHCNDF MLLGTEAVAL GRVGDDFWCT DPSGDPDGTF WLQGCHMVHC
    510 520 530 540 550
    AYNSLWMGAF IHPDWDMFQS THPCAAFHAA SRAVSGGPVY VSDAVGCHDF
    560 570 580 590 600
    DLLRRLALPD GTILRCERYA LPTRDCLFAD PLHDGKTMLK IWNVNKFSGV
    610 620 630 640 650
    LGAFNCQGGG WSREARRNMC AAGFSVPVTA RASPADVEWS HGGGGGDRFA
    660 670 680 690 700
    VYFVEARKLQ LLRRDESVEL TLEPFTYELL VVAPVRAIVS PELGIGFAPI
    710 720 730 740 750
    GLANMLNAGG AVQGFEAARK DGDVAAEVAV KGAGEMVAYS SARPRLCKVN
    760 770 780
    GQDAEFKYED GIVTVDVPWT GSSKKLSRVE YFY
    Length:783
    Mass (Da):85,533
    Last modified:December 7, 2004 - v1
    Checksum:i59BB443A5965DC1D
    GO

    Sequence cautioni

    The sequence BAF04051.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP003282 Genomic DNA. Translation: BAD68247.1.
    AP003339 Genomic DNA. Translation: BAD68321.1.
    AP008207 Genomic DNA. Translation: BAF04051.1. Sequence problems.
    CM000138 Genomic DNA. Translation: EAZ10704.1.
    RefSeqiNP_001042137.1. NM_001048672.1.
    UniGeneiOs.61038.

    Genome annotation databases

    GeneIDi4325200.
    KEGGiosa:4325200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP003282 Genomic DNA. Translation: BAD68247.1 .
    AP003339 Genomic DNA. Translation: BAD68321.1 .
    AP008207 Genomic DNA. Translation: BAF04051.1 . Sequence problems.
    CM000138 Genomic DNA. Translation: EAZ10704.1 .
    RefSeqi NP_001042137.1. NM_001048672.1.
    UniGenei Os.61038.

    3D structure databases

    ProteinModelPortali Q5VQG4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os01g07530.1.

    Proteomic databases

    PaxDbi Q5VQG4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4325200.
    KEGGi osa:4325200.

    Organism-specific databases

    Gramenei Q5VQG4.

    Phylogenomic databases

    eggNOGi NOG287560.
    InParanoidi Q5VQG4.
    KOi K06617.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare)."
      Li S., Li T., Kim W.-D., Kitaoka M., Yoshida S., Nakajima M., Kobayashi H.
      Biotechnol. Lett. 29:635-640(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    2. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiRFS_ORYSJ
    AccessioniPrimary (citable) accession number: Q5VQG4
    Secondary accession number(s): Q0JQC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: December 7, 2004
    Last modified: October 29, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3