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Q5VQG4

- RFS_ORYSJ

UniProt

Q5VQG4 - RFS_ORYSJ

Protein

Galactinol--sucrose galactosyltransferase

Gene

RFS

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors. Able to utilize sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose as acceptors. May also act as a glycoside hydrolase.1 Publication

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.1 Publication

    Enzyme regulationi

    Inhibited by Ag2+, Hg2+, Zn2+, p-chloromercuribenzoate (pCMB) and 1-deoxygalactonojirimycin.

    Kineticsi

      Vmax=25.6 nmol/sec/mg enzyme toward raffinose for the raffinose synthesis activity1 Publication

      Vmax=6.8 nmol/sec/mg enzyme toward p-nitrophenyl-alpha-D-galactoside for the hydrolysis activity1 Publication

      Vmax=8.5 nmol/sec/mg enzyme toward galactinol for the hydrolysis activity1 Publication

      Vmax=5.7 nmol/sec/mg enzyme toward raffinose for the hydrolysis activity1 Publication

      pH dependencei

      Optimum pH is 7.0.1 Publication

      Temperature dependencei

      Optimum temperature is 45 degrees Celsius.1 Publication

      GO - Molecular functioni

      1. galactinol-sucrose galactosyltransferase activity Source: Gramene

      GO - Biological processi

      1. carbohydrate metabolic process Source: UniProtKB-KW
      2. response to abscisic acid Source: EnsemblPlants/Gramene
      3. response to oxidative stress Source: EnsemblPlants/Gramene
      4. response to water deprivation Source: EnsemblPlants/Gramene

      Keywords - Molecular functioni

      Glycosyltransferase, Transferase

      Keywords - Biological processi

      Carbohydrate metabolism

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Galactinol--sucrose galactosyltransferase (EC:2.4.1.82)
      Alternative name(s):
      Raffinose synthase
      Gene namesi
      Name:RFS
      Ordered Locus Names:Os01g0170000, LOC_Os01g07530
      ORF Names:OJ1276_B06.38, OsJ_00538, P0583G08.2
      OrganismiOryza sativa subsp. japonica (Rice)
      Taxonomic identifieri39947 [NCBI]
      Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
      ProteomesiUP000000763: Chromosome 1

      Organism-specific databases

      GrameneiQ5VQG4.

      Subcellular locationi

      GO - Cellular componenti

      1. chloroplast Source: EnsemblPlants/Gramene

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Initiator methioninei1 – 11Removed1 Publication
      Chaini2 – 783782Galactinol--sucrose galactosyltransferasePRO_0000389255Add
      BLAST

      Proteomic databases

      PaxDbiQ5VQG4.

      Interactioni

      Protein-protein interaction databases

      STRINGi39947.LOC_Os01g07530.1.

      Structurei

      3D structure databases

      ProteinModelPortaliQ5VQG4.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Compositional bias

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Compositional biasi66 – 694Poly-Ala
      Compositional biasi273 – 2764Poly-Asp
      Compositional biasi642 – 6465Poly-Gly

      Sequence similaritiesi

      Belongs to the glycosyl hydrolases 36 family.Curated

      Phylogenomic databases

      eggNOGiNOG287560.
      KOiK06617.

      Family and domain databases

      Gene3Di3.20.20.70. 2 hits.
      InterProiIPR013785. Aldolase_TIM.
      IPR017853. Glycoside_hydrolase_SF.
      IPR008811. Glycosyl_hydrolases_36.
      [Graphical view]
      PfamiPF05691. Raffinose_syn. 1 hit.
      [Graphical view]
      SUPFAMiSSF51445. SSF51445. 3 hits.

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      Q5VQG4-1 [UniParc]FASTAAdd to Basket

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      MAPNLSKAKD DLIGDVVAVD GLIKPPRFTL KGKDLAVDGH PFLLDVPANI    50
      RLTPASTLVP NSDVPAAAAG SFLGFDAPAA KDRHVVPIGK LRDTRFMSIF 100
      RFKVWWTTHW VGTNGRDVEN ETQMMILDQS GTKSSPTGPR PYVLLLPIVE 150
      GPFRACLESG KAEDYVHMVL ESGSSTVRGS VFRSAVYLHA GDDPFDLVKD 200
      AMRVVRAHLG TFRLMEEKTP PPIVDKFGWC TWDAFYLKVH PEGVWEGVRR 250
      LADGGCPPGL VLIDDGWQSI CHDDDDLGSG AEGMNRTSAG EQMPCRLIKF 300
      QENYKFREYK GGMGGFVREM KAAFPTVEQV YVWHALCGYW GGLRPGAPGL 350
      PPAKVVAPRL SPGLQRTMED LAVDKIVNNG VGLVDPRRAR ELYEGLHSHL 400
      QASGIDGVKV DVIHLLEMVC EEYGGRVELA KAYFAGLTES VRRHFNGNGV 450
      IASMEHCNDF MLLGTEAVAL GRVGDDFWCT DPSGDPDGTF WLQGCHMVHC 500
      AYNSLWMGAF IHPDWDMFQS THPCAAFHAA SRAVSGGPVY VSDAVGCHDF 550
      DLLRRLALPD GTILRCERYA LPTRDCLFAD PLHDGKTMLK IWNVNKFSGV 600
      LGAFNCQGGG WSREARRNMC AAGFSVPVTA RASPADVEWS HGGGGGDRFA 650
      VYFVEARKLQ LLRRDESVEL TLEPFTYELL VVAPVRAIVS PELGIGFAPI 700
      GLANMLNAGG AVQGFEAARK DGDVAAEVAV KGAGEMVAYS SARPRLCKVN 750
      GQDAEFKYED GIVTVDVPWT GSSKKLSRVE YFY 783
      Length:783
      Mass (Da):85,533
      Last modified:December 7, 2004 - v1
      Checksum:i59BB443A5965DC1D
      GO

      Sequence cautioni

      The sequence BAF04051.1 differs from that shown. Reason: Erroneous gene model prediction.

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AP003282 Genomic DNA. Translation: BAD68247.1.
      AP003339 Genomic DNA. Translation: BAD68321.1.
      AP008207 Genomic DNA. Translation: BAF04051.1. Sequence problems.
      CM000138 Genomic DNA. Translation: EAZ10704.1.
      RefSeqiNP_001042137.1. NM_001048672.1.
      UniGeneiOs.61038.

      Genome annotation databases

      GeneIDi4325200.
      KEGGiosa:4325200.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AP003282 Genomic DNA. Translation: BAD68247.1 .
      AP003339 Genomic DNA. Translation: BAD68321.1 .
      AP008207 Genomic DNA. Translation: BAF04051.1 . Sequence problems.
      CM000138 Genomic DNA. Translation: EAZ10704.1 .
      RefSeqi NP_001042137.1. NM_001048672.1.
      UniGenei Os.61038.

      3D structure databases

      ProteinModelPortali Q5VQG4.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 39947.LOC_Os01g07530.1.

      Proteomic databases

      PaxDbi Q5VQG4.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      GeneIDi 4325200.
      KEGGi osa:4325200.

      Organism-specific databases

      Gramenei Q5VQG4.

      Phylogenomic databases

      eggNOGi NOG287560.
      KOi K06617.

      Family and domain databases

      Gene3Di 3.20.20.70. 2 hits.
      InterProi IPR013785. Aldolase_TIM.
      IPR017853. Glycoside_hydrolase_SF.
      IPR008811. Glycosyl_hydrolases_36.
      [Graphical view ]
      Pfami PF05691. Raffinose_syn. 1 hit.
      [Graphical view ]
      SUPFAMi SSF51445. SSF51445. 3 hits.
      ProtoNeti Search...

      Publicationsi

      1. "Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare)."
        Li S., Li T., Kim W.-D., Kitaoka M., Yoshida S., Nakajima M., Kobayashi H.
        Biotechnol. Lett. 29:635-640(2007) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      2. "The genome sequence and structure of rice chromosome 1."
        Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
        , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
        Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: cv. Nipponbare.
      3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: cv. Nipponbare.
      4. "The rice annotation project database (RAP-DB): 2008 update."
        The rice annotation project (RAP)
        Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
        Cited for: GENOME REANNOTATION.
        Strain: cv. Nipponbare.
      5. "The genomes of Oryza sativa: a history of duplications."
        Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
        , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
        PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: cv. Nipponbare.

      Entry informationi

      Entry nameiRFS_ORYSJ
      AccessioniPrimary (citable) accession number: Q5VQG4
      Secondary accession number(s): Q0JQC7
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: November 24, 2009
      Last sequence update: December 7, 2004
      Last modified: October 1, 2014
      This is version 48 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programPlant Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Direct protein sequencing, Reference proteome

      Documents

      1. Oryza sativa (rice)
        Index of Oryza sativa entries and their corresponding gene designations
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3