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Protein

Histone-lysine N-methyltransferase CLF

Gene

CLF

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development (Probable). Involved in the regulation of flowering. Represses flowering under long day (LD) conditions. Regulates the trimethylation on histone H3 'Lys-27' (H3K27me3) of the flowering regulators MADS14, MADS15, RFT1, EHD1, HD3A and LF (PubMed:25400654).1 Publication1 Publication

Miscellaneous

Over-expression and down-regulation of CLF respectively, represses and promotes flowering in long day (LD), but has no effect in short day (SD).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei861S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Methyltransferase, Transferase
Biological processDifferentiation, Flowering, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-OSA-212300 PRC2 methylates histones and DNA
R-OSA-2559580 Oxidative Stress Induced Senescence
R-OSA-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase CLFCurated (EC:2.1.1.43PROSITE-ProRule annotation)
Short name:
OsCLF1 Publication
Alternative name(s):
Protein SET DOMAIN GROUP 711Curated
SET family protein 24Curated
Short name:
OsSET241 Publication
Gene namesi
Name:CLF1 Publication
Synonyms:SDG7111 Publication
Ordered Locus Names:Os06g0275500Imported, LOC_Os06g16390Curated
ORF Names:P0038C05.29Imported, P0676F10.40Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004444651 – 896Histone-lysine N-methyltransferase CLFAdd BLAST896

Expressioni

Tissue specificityi

Widely expressed (PubMed:19825651). Highly expressed in young panicle (PubMed:23762371).2 Publications

Interactioni

Subunit structurei

Interacts with FIE1 (PubMed:23150632). Component of the polycomb repressive complex 2 (PRC2), composed of the core PRC2 components FIE2, EMF2B and EZ1. PRC2 methylates 'Lys-27' residues of histone H3 (H3K27me3), leading to transcriptional repression of the affected target gene (PubMed:23505380).2 Publications

Protein-protein interaction databases

STRINGi39947.LOC_Os06g16390.1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini633 – 732CXCPROSITE-ProRule annotationAdd BLAST100
Domaini747 – 862SETPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1079 Eukaryota
COG2940 LUCA
KOiK11430
OMAiCAVNSRE
OrthoDBiEOG09360260

Family and domain databases

InterProiView protein in InterPro
IPR026489 CXC_dom
IPR025778 Hist-Lys_N-MeTrfase_EZ
IPR001214 SET_dom
IPR033467 Tesmin/TSO1-like_CXC
PANTHERiPTHR22884:SF237 PTHR22884:SF237, 1 hit
PfamiView protein in Pfam
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM01114 CXC, 1 hit
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS51633 CXC, 1 hit
PS51576 SAM_MT43_EZ, 1 hit
PS50280 SET, 1 hit

Sequencei

Sequence statusi: Complete.

Q5VN06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSRNEPM FCEEGSSESG YVLCVIDSLK KKITSDRFVY IQKRVEENSI
60 70 80 90 100
KLSPITLHSH NLSKNRQTST SNSTDLVSNL LTKRKEDALC AVNSRESSPD
110 120 130 140 150
ESEGANCQDE CSSTVIVGGN LSARNSVRPI RLPEVATLPP YTTWIFLDRN
160 170 180 190 200
QRMQEDQSVL GRRRIYYDTN CGEALICSDS EDEAVEDEEE KKEFKDSEDC
210 220 230 240 250
IIRMTIQECG MSDAVLETLA RDIERAPDDI KARYEILQGE KPEGSSKKVS
260 270 280 290 300
ELNVKMEDVY GDKDLDAALD SFDNLFCRRC LVFDCKLHGC SQDLVFPTEK
310 320 330 340 350
QAPLCSSDEG TPCGIHCYKL VSKPDAIMEI DSHLLVDVEE PTSDNLKDQI
360 370 380 390 400
GSNKKKLGSS GQKTKSQQSE SSSTARVSSE SSESEVQLLS NKSPQHSPGL
410 420 430 440 450
SKNKLGAKGG IKKSTNRRIA ERILMSVKKG QQEMSPDSNS IVNGCHWPRD
460 470 480 490 500
MKLRSDTRSG IKDSVVSSQC NSPSTRSFRK KGTLQMENNS SFVDAQSDSM
510 520 530 540 550
EDTNNEHSAT DGCDSSRKEE CVDESICRQE AHGRSWKVIE QGLLLKGLEI
560 570 580 590 600
FGKNSCLIAR NLLGGMKTCT DVFQYMNYIE NSSASGALSG VDSLVKGYMK
610 620 630 640 650
GNELRTRSRF VRRRGRVRRL KYTWKTAGYH FIRKRITERK DQPCRQYTPC
660 670 680 690 700
GCQSACGKQC PCLTNGTCCE KYCGCPKMCK NRFRGCHCAK SQCRSRQCPC
710 720 730 740 750
FAADRECDPD VCRNCWVGCG DGTLGVPNQR GDNYECRNMK LLLKQQQRVL
760 770 780 790 800
LGRSDVSGWG AFLKNSVGKH EYLGEYTGEL ISHKEADKRG KIYDRENSSF
810 820 830 840 850
LFNLNNEYVL DAYRMGDKLK FANHSPDPNC YAKVIMVAGD HRVGIFAKER
860 870 880 890
ISAGEELFYD YRYEPDRAPA WARKPEGPGA KDDAQPSTGR AKKLAH
Length:896
Mass (Da):100,296
Last modified:December 7, 2004 - v1
Checksum:i7DCE38E04E7505DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003044 Genomic DNA Translation: BAD68028.1
AP005813 Genomic DNA Translation: BAD69169.1
AP008212 Genomic DNA Translation: BAF19293.1
AP014962 Genomic DNA Translation: BAS97226.1
RefSeqiXP_015644234.1, XM_015788748.1
UniGeneiOs.44686

Genome annotation databases

EnsemblPlantsiOs06t0275500-01; Os06t0275500-01; Os06g0275500
GeneIDi4340748
GrameneiOs06t0275500-01; Os06t0275500-01; Os06g0275500
KEGGiosa:4340748

Similar proteinsi

Entry informationi

Entry nameiCLF_ORYSJ
AccessioniPrimary (citable) accession number: Q5VN06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2018
Last sequence update: December 7, 2004
Last modified: June 20, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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